<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2021 Workiva-->
<!--r:7d422e42-b86f-498f-8bfa-6275a5368a99,g:aeb4ff85-211d-4e10-80f0-b77dd9612adb-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.xpo.com/role/CoverPage" xlink:type="simple" xlink:href="xpo-20201231.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/CoverPage" xlink:type="extended" id="i555834bcaf8844feb6fa986ec9b6905a_CoverPage"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedBalanceSheets" xlink:type="extended" id="i50d121f172b64be695fbb23d3eb7c350_ConsolidatedBalanceSheets"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="extended" id="iacb488c574b54e178a2e3bba75c34b37_ConsolidatedBalanceSheetsParenthetical"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedStatementsofIncome" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedStatementsofIncome"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedStatementsofIncome" xlink:type="extended" id="i5edddfbef0b447138b4597ab1926de46_ConsolidatedStatementsofIncome"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedStatementsofComprehensiveIncome" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedStatementsofComprehensiveIncome"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedStatementsofComprehensiveIncome" xlink:type="extended" id="if63e5da45af04a09855d030afc349091_ConsolidatedStatementsofComprehensiveIncome"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedStatementsofComprehensiveIncomeParenthetical" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedStatementsofComprehensiveIncomeParenthetical"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedStatementsofComprehensiveIncomeParenthetical" xlink:type="extended" id="i4f6bd393cd564e4bb9a57cef93a54d46_ConsolidatedStatementsofComprehensiveIncomeParenthetical"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended" id="i25b864980cc649c8b613debe65302ccb_ConsolidatedStatementsofCashFlows"/>
  <link:roleRef roleURI="http://www.xpo.com/role/ConsolidatedStatementsofChangesinEquity" xlink:type="simple" xlink:href="xpo-20201231.xsd#ConsolidatedStatementsofChangesinEquity"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/ConsolidatedStatementsofChangesinEquity" xlink:type="extended" id="i7dcde98b243c4e1c8add12e724c6329e_ConsolidatedStatementsofChangesinEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_5b1c2842-6c49-4b05-a5b9-a151d4e89be7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_5b1c2842-6c49-4b05-a5b9-a151d4e89be7" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_5aecae69-7b16-42fd-9137-d3e17afee81e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_SharesIssued_5aecae69-7b16-42fd-9137-d3e17afee81e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_9b73cf18-b897-4e22-8919-d614989f4920" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_9b73cf18-b897-4e22-8919-d614989f4920" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_df299da8-df0f-40cf-ae3c-13b5193cf97a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_ProfitLoss_df299da8-df0f-40cf-ae3c-13b5193cf97a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_a4781f3d-26bf-4dea-af66-c54c99080bba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_a4781f3d-26bf-4dea-af66-c54c99080bba" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_StockIssuedDuringPeriodSharesSharebasedCompensationExercisedandVested_afdf62ec-d567-4325-8718-1ce03fe9984d" xlink:href="xpo-20201231.xsd#xpo_StockIssuedDuringPeriodSharesSharebasedCompensationExercisedandVested"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_xpo_StockIssuedDuringPeriodSharesSharebasedCompensationExercisedandVested_afdf62ec-d567-4325-8718-1ce03fe9984d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_StockIssuedDuringPeriodValueSharebasedCompensationExercisedandVested_7d37222a-786f-499e-a4e9-add68325f2b2" xlink:href="xpo-20201231.xsd#xpo_StockIssuedDuringPeriodValueSharebasedCompensationExercisedandVested"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_xpo_StockIssuedDuringPeriodValueSharebasedCompensationExercisedandVested_7d37222a-786f-499e-a4e9-add68325f2b2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_938ee05b-fecf-4f4d-99a6-021745f9e875" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_938ee05b-fecf-4f4d-99a6-021745f9e875" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestDecreaseFromRedemptions_ba8a6ef1-4b1c-4fe8-a4b3-be059f5035fe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MinorityInterestDecreaseFromRedemptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_MinorityInterestDecreaseFromRedemptions_ba8a6ef1-4b1c-4fe8-a4b3-be059f5035fe" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_f980e72d-afd6-4ccd-9e23-7be5d2293ea5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_f980e72d-afd6-4ccd-9e23-7be5d2293ea5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_5176477f-4757-4785-934d-9fd44219da4d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_5176477f-4757-4785-934d-9fd44219da4d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_StockholdersEquityConversionOfPreferredStock_ab69aaf1-1a9a-4eb3-9c4b-fc6366ac02da" xlink:href="xpo-20201231.xsd#xpo_StockholdersEquityConversionOfPreferredStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_xpo_StockholdersEquityConversionOfPreferredStock_ab69aaf1-1a9a-4eb3-9c4b-fc6366ac02da" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_b29f851f-d670-4542-8775-b4a2e9dd2664" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_b29f851f-d670-4542-8775-b4a2e9dd2664" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_91468f46-fac4-46a5-9a82-bcf92f91bf20" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_91468f46-fac4-46a5-9a82-bcf92f91bf20" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedAndRetiredDuringPeriodShares_ac5cdf9f-5d5b-4abf-a5be-09d064db491c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchasedAndRetiredDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockRepurchasedAndRetiredDuringPeriodShares_ac5cdf9f-5d5b-4abf-a5be-09d064db491c" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedAndRetiredDuringPeriodValue_c159c197-5db0-4af3-9dcb-ee09394044f0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchasedAndRetiredDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockRepurchasedAndRetiredDuringPeriodValue_c159c197-5db0-4af3-9dcb-ee09394044f0" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCash_474f9645-eb64-4b10-aa5e-0897c8b6184c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DividendsCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_DividendsCash_474f9645-eb64-4b10-aa5e-0897c8b6184c" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_940633f9-6ae0-43bd-98a3-16200aface76" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationAndExerciseOfStockOptions_940633f9-6ae0-43bd-98a3-16200aface76" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityOtherShares_0355c3d4-8362-4dc4-a973-efac5e74a9db" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityOtherShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockholdersEquityOtherShares_0355c3d4-8362-4dc4-a973-efac5e74a9db" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityOther_0a68c849-009d-4a42-b527-948f193272fc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_07017c24-d2c4-4a41-8064-af70830cc0ed" xlink:to="loc_us-gaap_StockholdersEquityOther_0a68c849-009d-4a42-b527-948f193272fc" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssued_7b9dd693-f211-4e21-aa20-84c7a12851fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharesIssued"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_42b02db2-b9fc-4383-b0be-7acb97db8e5d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_60ff9603-65f2-4b28-97a8-f0e36a4c021f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_5b1c2842-6c49-4b05-a5b9-a151d4e89be7" xlink:to="loc_us-gaap_StatementTable_60ff9603-65f2-4b28-97a8-f0e36a4c021f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_fde38b82-b9f0-4af8-becd-4c0ad317868b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_60ff9603-65f2-4b28-97a8-f0e36a4c021f" xlink:to="loc_us-gaap_StatementClassOfStockAxis_fde38b82-b9f0-4af8-becd-4c0ad317868b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_fde38b82-b9f0-4af8-becd-4c0ad317868b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fde38b82-b9f0-4af8-becd-4c0ad317868b" xlink:to="loc_us-gaap_ClassOfStockDomain_fde38b82-b9f0-4af8-becd-4c0ad317868b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_d8ccdd31-2152-46b8-a9aa-fb7dd7e9486b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_fde38b82-b9f0-4af8-becd-4c0ad317868b" xlink:to="loc_us-gaap_ClassOfStockDomain_d8ccdd31-2152-46b8-a9aa-fb7dd7e9486b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesAPreferredStockMember_f93121f8-9e4b-487f-9f1a-64d1b0f14a6c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SeriesAPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_d8ccdd31-2152-46b8-a9aa-fb7dd7e9486b" xlink:to="loc_us-gaap_SeriesAPreferredStockMember_f93121f8-9e4b-487f-9f1a-64d1b0f14a6c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_60ff9603-65f2-4b28-97a8-f0e36a4c021f" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7" xlink:to="loc_us-gaap_EquityComponentDomain_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6bf61c42-4897-416d-9f7f-a3cffdb4bbd7" xlink:to="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_651ef5ca-1e60-4ebc-bde7-33a418548819" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_PreferredStockMember_651ef5ca-1e60-4ebc-bde7-33a418548819" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_6f4ad74a-f2d6-4039-b2b7-e9a856cfcd9d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_CommonStockMember_6f4ad74a-f2d6-4039-b2b7-e9a856cfcd9d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_e742b9e6-9407-4496-be54-186f7144e288" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_e742b9e6-9407-4496-be54-186f7144e288" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_a6524732-515d-419e-83c4-508361b88973" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_RetainedEarningsMember_a6524732-515d-419e-83c4-508361b88973" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_a61bbea1-1a52-4c6a-a8d7-1faf3fc84189" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_a61bbea1-1a52-4c6a-a8d7-1faf3fc84189" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ParentMember_a1bb37dd-3780-472c-be14-4da642dcb246" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_ParentMember_a1bb37dd-3780-472c-be14-4da642dcb246" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncontrollingInterestMember_92c9d1a3-3d09-44c5-a0c2-161395fd01ae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_7d096db7-2f24-45aa-91aa-8d525ec12446" xlink:to="loc_us-gaap_NoncontrollingInterestMember_92c9d1a3-3d09-44c5-a0c2-161395fd01ae" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/Organization" xlink:type="simple" xlink:href="xpo-20201231.xsd#Organization"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/Organization" xlink:type="extended" id="ie7e7789aeb6047c382c83e931d75a167_Organization"/>
  <link:roleRef roleURI="http://www.xpo.com/role/OrganizationNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#OrganizationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/OrganizationNarrativeDetails" xlink:type="extended" id="i0db1337e2d2a4a95b5ed3933b3335bdc_OrganizationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetLineItems_9be9f00b-0dc1-431e-b294-8df3335677f4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_4562ec3e-88a6-48dd-913a-7130bc8e62b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetLineItems_9be9f00b-0dc1-431e-b294-8df3335677f4" xlink:to="loc_us-gaap_NumberOfReportableSegments_4562ec3e-88a6-48dd-913a-7130bc8e62b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SpinOffPercentageOfOperatingSegment_24bd7b8f-9232-48cb-b3b7-8b78621c5fe4" xlink:href="xpo-20201231.xsd#xpo_SpinOffPercentageOfOperatingSegment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetLineItems_9be9f00b-0dc1-431e-b294-8df3335677f4" xlink:to="loc_xpo_SpinOffPercentageOfOperatingSegment_24bd7b8f-9232-48cb-b3b7-8b78621c5fe4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetTable_79706134-a32e-40b2-80fd-cefcb8a04758" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetLineItems_9be9f00b-0dc1-431e-b294-8df3335677f4" xlink:to="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetTable_79706134-a32e-40b2-80fd-cefcb8a04758" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_b049ad26-95e9-4046-a061-3b2e98fa8074" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConsolidationLessThanWhollyOwnedSubsidiaryParentOwnershipInterestEffectsOfChangesNetTable_79706134-a32e-40b2-80fd-cefcb8a04758" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_b049ad26-95e9-4046-a061-3b2e98fa8074" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_b049ad26-95e9-4046-a061-3b2e98fa8074_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_b049ad26-95e9-4046-a061-3b2e98fa8074" xlink:to="loc_us-gaap_SegmentDomain_b049ad26-95e9-4046-a061-3b2e98fa8074_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_f84cb23f-a617-4016-abee-c986a4a01aed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_b049ad26-95e9-4046-a061-3b2e98fa8074" xlink:to="loc_us-gaap_SegmentDomain_f84cb23f-a617-4016-abee-c986a4a01aed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LogisticsMember_06b545a9-0eb7-4b30-96ea-3ad8d22ffe51" xlink:href="xpo-20201231.xsd#xpo_LogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_f84cb23f-a617-4016-abee-c986a4a01aed" xlink:to="loc_xpo_LogisticsMember_06b545a9-0eb7-4b30-96ea-3ad8d22ffe51" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPolicies" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPolicies"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPolicies" xlink:type="extended" id="i383c3a0ea1714fd7b4dc175bea1d940e_BasisofPresentationandSignificantAccountingPolicies"/>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesPolicies" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesPolicies"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesPolicies" xlink:type="extended" id="i79fa91c5dc664909a6b034c3c0f4a261_BasisofPresentationandSignificantAccountingPoliciesPolicies"/>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesTables" xlink:type="extended" id="i66bf4c2d452640ebae43f037884803ea_BasisofPresentationandSignificantAccountingPoliciesTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesConsolidationDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesConsolidationDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesConsolidationDetails" xlink:type="extended" id="i9920e1be9a914f0992f6fbe7038fbdda_BasisofPresentationandSignificantAccountingPoliciesConsolidationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestLineItems_0e00c93d-75c4-4db4-80ba-9262c251538e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MinorityInterestLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_67ef3d1e-09c5-4240-aadb-8954479726bc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MinorityInterestLineItems_0e00c93d-75c4-4db4-80ba-9262c251538e" xlink:to="loc_us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_67ef3d1e-09c5-4240-aadb-8954479726bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestTable_8651d053-1924-4b62-a37d-ffa72c36dd38" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MinorityInterestTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_MinorityInterestLineItems_0e00c93d-75c4-4db4-80ba-9262c251538e" xlink:to="loc_us-gaap_MinorityInterestTable_8651d053-1924-4b62-a37d-ffa72c36dd38" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_59e42067-c678-40d4-866a-3307756c158a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_MinorityInterestTable_8651d053-1924-4b62-a37d-ffa72c36dd38" xlink:to="loc_srt_OwnershipAxis_59e42067-c678-40d4-866a-3307756c158a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_59e42067-c678-40d4-866a-3307756c158a_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_59e42067-c678-40d4-866a-3307756c158a" xlink:to="loc_srt_OwnershipDomain_59e42067-c678-40d4-866a-3307756c158a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_a6a5a1f9-f087-44e6-904f-ea89f85d1d06" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_59e42067-c678-40d4-866a-3307756c158a" xlink:to="loc_srt_OwnershipDomain_a6a5a1f9-f087-44e6-904f-ea89f85d1d06" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_NDMember_b8412c52-4a01-4096-9ddd-57a39a47b52d" xlink:href="xpo-20201231.xsd#xpo_NDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_a6a5a1f9-f087-44e6-904f-ea89f85d1d06" xlink:to="loc_xpo_NDMember_b8412c52-4a01-4096-9ddd-57a39a47b52d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesCashCashEquivalentsandRestrictedCashDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesCashCashEquivalentsandRestrictedCashDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesCashCashEquivalentsandRestrictedCashDetails" xlink:type="extended" id="if952381b57d3456084b02337051fb55d_BasisofPresentationandSignificantAccountingPoliciesCashCashEquivalentsandRestrictedCashDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_8a0bd8e1-51a6-4412-9085-8b07736bdce5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashNoncurrent_f0aea3da-823e-43ee-ad0b-a955027228c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_8a0bd8e1-51a6-4412-9085-8b07736bdce5" xlink:to="loc_us-gaap_RestrictedCashNoncurrent_f0aea3da-823e-43ee-ad0b-a955027228c3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_770bd17a-78ed-449a-a09e-fa00a45a18bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_8a0bd8e1-51a6-4412-9085-8b07736bdce5" xlink:to="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_770bd17a-78ed-449a-a09e-fa00a45a18bb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_78e38b1e-0577-4fa8-b68b-fb94e1686c02" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_770bd17a-78ed-449a-a09e-fa00a45a18bb" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_78e38b1e-0577-4fa8-b68b-fb94e1686c02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_78e38b1e-0577-4fa8-b68b-fb94e1686c02_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_78e38b1e-0577-4fa8-b68b-fb94e1686c02" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_78e38b1e-0577-4fa8-b68b-fb94e1686c02_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_89395519-80da-4a98-bc1a-34db0d7d7be5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_78e38b1e-0577-4fa8-b68b-fb94e1686c02" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_89395519-80da-4a98-bc1a-34db0d7d7be5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncurrentAssetsMember_da81acc1-7eaa-4974-87a9-caad0ebdfc61" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNoncurrentAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_89395519-80da-4a98-bc1a-34db0d7d7be5" xlink:to="loc_us-gaap_OtherNoncurrentAssetsMember_da81acc1-7eaa-4974-87a9-caad0ebdfc61" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableandAllowanceforDoubtfulAccountsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableandAllowanceforDoubtfulAccountsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableandAllowanceforDoubtfulAccountsDetails" xlink:type="extended" id="i72981beef4c842da8eef5f185a579e65_BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableandAllowanceforDoubtfulAccountsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_b70e11c3-e6c4-4691-a5fa-a4f1afa49f1c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward_b374fae0-fc38-404f-8717-c3789c1157a0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_b70e11c3-e6c4-4691-a5fa-a4f1afa49f1c" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward_b374fae0-fc38-404f-8717-c3789c1157a0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_6f9022ef-9a4f-4504-94f2-e112770560f7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward_b374fae0-fc38-404f-8717-c3789c1157a0" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_6f9022ef-9a4f-4504-94f2-e112770560f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_d44ea89b-4dfd-47aa-b622-3830da6f12d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward_b374fae0-fc38-404f-8717-c3789c1157a0" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_d44ea89b-4dfd-47aa-b622-3830da6f12d6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivablePeriodIncreaseDecrease_673048bd-3204-4a3b-aa45-4d8a0900c22e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivablePeriodIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AllowanceForDoubtfulAccountsReceivableRollforward_b374fae0-fc38-404f-8717-c3789c1157a0" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivablePeriodIncreaseDecrease_673048bd-3204-4a3b-aa45-4d8a0900c22e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_f2cc068a-f9fe-4bb3-91c5-dbdd82cc4296" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_e89995f8-2333-4cef-96c6-e84a101ade28" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_b70e11c3-e6c4-4691-a5fa-a4f1afa49f1c" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_e89995f8-2333-4cef-96c6-e84a101ade28" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_4739a82c-3568-42b3-b90a-2fe7ceeb0e77" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_e89995f8-2333-4cef-96c6-e84a101ade28" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_4739a82c-3568-42b3-b90a-2fe7ceeb0e77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_4739a82c-3568-42b3-b90a-2fe7ceeb0e77_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_4739a82c-3568-42b3-b90a-2fe7ceeb0e77" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_4739a82c-3568-42b3-b90a-2fe7ceeb0e77_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_5943586d-d765-4815-b168-96ed3316eda6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_4739a82c-3568-42b3-b90a-2fe7ceeb0e77" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_5943586d-d765-4815-b168-96ed3316eda6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_b7500c93-e47c-4dd4-84f2-adc2bd34b195" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_5943586d-d765-4815-b168-96ed3316eda6" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_b7500c93-e47c-4dd4-84f2-adc2bd34b195" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableSecuritizationandFactoringProgramsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableSecuritizationandFactoringProgramsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableSecuritizationandFactoringProgramsDetails" xlink:type="extended" id="i7660120b036640739e17aecb13147a05_BasisofPresentationandSignificantAccountingPoliciesAccountsReceivableSecuritizationandFactoringProgramsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableInterestEntityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_NumberOfBanksTradeReceivableSecuritizationProgram_f44e1784-de19-4283-8254-4c582dc98976" xlink:href="xpo-20201231.xsd#xpo_NumberOfBanksTradeReceivableSecuritizationProgram"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_xpo_NumberOfBanksTradeReceivableSecuritizationProgram_f44e1784-de19-4283-8254-4c582dc98976" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_e6382038-65b3-49f6-80ce-99f2e8be276a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_us-gaap_LongTermDebtFairValue_e6382038-65b3-49f6-80ce-99f2e8be276a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_c847e002-e830-44bc-a437-cf04145fdc08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_c847e002-e830-44bc-a437-cf04145fdc08" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_5800ad4c-83c0-4404-b162-7b0b165e45d4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_5800ad4c-83c0-4404-b162-7b0b165e45d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtWeightedAverageInterestRate_f0fad041-4e54-456a-bf1f-842001250ef4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtWeightedAverageInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_us-gaap_DebtWeightedAverageInterestRate_f0fad041-4e54-456a-bf1f-842001250ef4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfVariableInterestEntitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_VariableInterestEntityLineItems_298367f7-7e33-47a2-aeca-93c8eff31740" xlink:to="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_edb4ac7f-7784-4c56-9bf6-f4860b095443" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_edb4ac7f-7784-4c56-9bf6-f4860b095443" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_edb4ac7f-7784-4c56-9bf6-f4860b095443_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_edb4ac7f-7784-4c56-9bf6-f4860b095443" xlink:to="loc_us-gaap_RelatedPartyDomain_edb4ac7f-7784-4c56-9bf6-f4860b095443_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_8244d930-a720-4abf-bc05-d84bd9d29d72" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_edb4ac7f-7784-4c56-9bf6-f4860b095443" xlink:to="loc_us-gaap_RelatedPartyDomain_8244d930-a720-4abf-bc05-d84bd9d29d72" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_cf8c81c1-85e9-40f2-9cbe-7a83b2e14a1d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_8244d930-a720-4abf-bc05-d84bd9d29d72" xlink:to="loc_srt_AffiliatedEntityMember_cf8c81c1-85e9-40f2-9cbe-7a83b2e14a1d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_4166150a-c856-4734-9a42-c028749bbe3c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_4166150a-c856-4734-9a42-c028749bbe3c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_4166150a-c856-4734-9a42-c028749bbe3c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_4166150a-c856-4734-9a42-c028749bbe3c" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_4166150a-c856-4734-9a42-c028749bbe3c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_55d3d720-9263-4cec-8b46-5af14945a814" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_4166150a-c856-4734-9a42-c028749bbe3c" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_55d3d720-9263-4cec-8b46-5af14945a814" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TradeReceivablesSecuritizationProgramTwoMember_24aff74b-2b5d-40a2-b04e-a9a010ccac72" xlink:href="xpo-20201231.xsd#xpo_TradeReceivablesSecuritizationProgramTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_55d3d720-9263-4cec-8b46-5af14945a814" xlink:to="loc_xpo_TradeReceivablesSecuritizationProgramTwoMember_24aff74b-2b5d-40a2-b04e-a9a010ccac72" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_d3b25cad-400b-4778-b040-5067aad17cbd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:to="loc_srt_CounterpartyNameAxis_d3b25cad-400b-4778-b040-5067aad17cbd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_d3b25cad-400b-4778-b040-5067aad17cbd_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_d3b25cad-400b-4778-b040-5067aad17cbd" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_d3b25cad-400b-4778-b040-5067aad17cbd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_63e25fa8-f374-44d7-b1ec-b6b616071f22" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_d3b25cad-400b-4778-b040-5067aad17cbd" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_63e25fa8-f374-44d7-b1ec-b6b616071f22" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_XPOCollectionsDesignatedActivityCompanyLimitedMember_20af2228-3663-47b3-a6a1-e48451c6de36" xlink:href="xpo-20201231.xsd#xpo_XPOCollectionsDesignatedActivityCompanyLimitedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_63e25fa8-f374-44d7-b1ec-b6b616071f22" xlink:to="loc_xpo_XPOCollectionsDesignatedActivityCompanyLimitedMember_20af2228-3663-47b3-a6a1-e48451c6de36" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_857c6b30-3c65-43dc-821b-f990e89521a1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:to="loc_us-gaap_DebtInstrumentAxis_857c6b30-3c65-43dc-821b-f990e89521a1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_857c6b30-3c65-43dc-821b-f990e89521a1_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_857c6b30-3c65-43dc-821b-f990e89521a1" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_857c6b30-3c65-43dc-821b-f990e89521a1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f3c85470-2dd9-4522-b1e0-0980c991e034" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_857c6b30-3c65-43dc-821b-f990e89521a1" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f3c85470-2dd9-4522-b1e0-0980c991e034" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EuropeanTradeSecuritizationProgramMember_af9a4ec6-fdb8-4b34-b982-df4e3fb78b7b" xlink:href="xpo-20201231.xsd#xpo_EuropeanTradeSecuritizationProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f3c85470-2dd9-4522-b1e0-0980c991e034" xlink:to="loc_xpo_EuropeanTradeSecuritizationProgramMember_af9a4ec6-fdb8-4b34-b982-df4e3fb78b7b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_5c35010b-e1b1-4737-bdd4-9c46566c3060" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfVariableInterestEntitiesTable_b187a0e4-7baf-485c-bf9e-33f1ceebe20d" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_5c35010b-e1b1-4737-bdd4-9c46566c3060" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_5c35010b-e1b1-4737-bdd4-9c46566c3060_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_5c35010b-e1b1-4737-bdd4-9c46566c3060" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_5c35010b-e1b1-4737-bdd4-9c46566c3060_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5207ccbd-5ed4-43d9-a922-b4fc370f79f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_5c35010b-e1b1-4737-bdd4-9c46566c3060" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5207ccbd-5ed4-43d9-a922-b4fc370f79f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_fb52debe-10e9-4ed4-ad40-26ad9c2228a6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_5207ccbd-5ed4-43d9-a922-b4fc370f79f1" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_fb52debe-10e9-4ed4-ad40-26ad9c2228a6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_1613a8f8-47a4-404e-927e-36a7f3596098" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_fb52debe-10e9-4ed4-ad40-26ad9c2228a6" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_1613a8f8-47a4-404e-927e-36a7f3596098" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesPropertyandEquipmentDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesPropertyandEquipmentDetails" xlink:type="extended" id="i22ad3e1b55874b19a10311eecda51c0f_BasisofPresentationandSignificantAccountingPoliciesPropertyandEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_64384a7d-1366-4d40-9d02-09d6ec3b3402" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_9cb1a0b6-91bb-439c-9692-d4d362e2cefe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_64384a7d-1366-4d40-9d02-09d6ec3b3402" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_9cb1a0b6-91bb-439c-9692-d4d362e2cefe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_12bed6a1-bde1-46d5-bdaa-53522859e462" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_64384a7d-1366-4d40-9d02-09d6ec3b3402" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_12bed6a1-bde1-46d5-bdaa-53522859e462" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8b505187-fd66-4d6c-994a-9ff1b1e06c63" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_12bed6a1-bde1-46d5-bdaa-53522859e462" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8b505187-fd66-4d6c-994a-9ff1b1e06c63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_8b505187-fd66-4d6c-994a-9ff1b1e06c63_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8b505187-fd66-4d6c-994a-9ff1b1e06c63" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_8b505187-fd66-4d6c-994a-9ff1b1e06c63_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_8b505187-fd66-4d6c-994a-9ff1b1e06c63" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_7336d07a-7b4b-486f-8675-6af896d29b5a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_7336d07a-7b4b-486f-8675-6af896d29b5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VehiclesMember_5e809faf-1851-4517-afca-61200a6a6890" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:to="loc_us-gaap_VehiclesMember_5e809faf-1851-4517-afca-61200a6a6890" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RailroadTransportationEquipmentMember_b25f8784-79ec-4796-bb4a-4f8d33b61779" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RailroadTransportationEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:to="loc_us-gaap_RailroadTransportationEquipmentMember_b25f8784-79ec-4796-bb4a-4f8d33b61779" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MachineryAndEquipmentMember_195ccf50-3c30-4871-a0d1-79e7933bf5c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MachineryAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:to="loc_us-gaap_MachineryAndEquipmentMember_195ccf50-3c30-4871-a0d1-79e7933bf5c4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ComputerSoftwareMember_4cca8516-de32-432d-bd86-9b158899c87a" xlink:href="xpo-20201231.xsd#xpo_ComputerSoftwareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c507b9de-13b6-4f54-a09c-1772864e6459" xlink:to="loc_xpo_ComputerSoftwareMember_4cca8516-de32-432d-bd86-9b158899c87a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_8aea2e16-96ab-4db6-8d70-a43b9fd535e1" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_12bed6a1-bde1-46d5-bdaa-53522859e462" xlink:to="loc_srt_RangeAxis_8aea2e16-96ab-4db6-8d70-a43b9fd535e1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8aea2e16-96ab-4db6-8d70-a43b9fd535e1_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_8aea2e16-96ab-4db6-8d70-a43b9fd535e1" xlink:to="loc_srt_RangeMember_8aea2e16-96ab-4db6-8d70-a43b9fd535e1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_c888cbe0-e885-4668-85b6-962dc7ab2c04" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_8aea2e16-96ab-4db6-8d70-a43b9fd535e1" xlink:to="loc_srt_RangeMember_c888cbe0-e885-4668-85b6-962dc7ab2c04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_b6b0cbcc-7416-421b-97d1-b37505400f7f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c888cbe0-e885-4668-85b6-962dc7ab2c04" xlink:to="loc_srt_MinimumMember_b6b0cbcc-7416-421b-97d1-b37505400f7f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_9fe785dd-4dab-41dd-b434-5656aca3c7d7" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c888cbe0-e885-4668-85b6-962dc7ab2c04" xlink:to="loc_srt_MaximumMember_9fe785dd-4dab-41dd-b434-5656aca3c7d7" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesGoodwillDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesGoodwillDetails" xlink:type="extended" id="iefc104db65b04697b90ad0eb2164e5f9_BasisofPresentationandSignificantAccountingPoliciesGoodwillDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesIntangibleAssetsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesIntangibleAssetsDetails" xlink:type="extended" id="i345e95d4bce74d8698cb76c480ff58fa_BasisofPresentationandSignificantAccountingPoliciesIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeLineItems_5c02f311-a069-448a-bd19-18850a55e3ca" xlink:href="xpo-20201231.xsd#xpo_FiniteLivedIntangibleAssetUsefulLifeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_082f86fc-4df1-4e8c-8bfe-1331fbcf2913" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeLineItems_5c02f311-a069-448a-bd19-18850a55e3ca" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_082f86fc-4df1-4e8c-8bfe-1331fbcf2913" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeTable_9070043b-bbf0-4447-ad1d-c643fddd8640" xlink:href="xpo-20201231.xsd#xpo_FiniteLivedIntangibleAssetUsefulLifeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeLineItems_5c02f311-a069-448a-bd19-18850a55e3ca" xlink:to="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeTable_9070043b-bbf0-4447-ad1d-c643fddd8640" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_dcebb986-d65d-4663-82cd-87129e77c80b" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeTable_9070043b-bbf0-4447-ad1d-c643fddd8640" xlink:to="loc_srt_RangeAxis_dcebb986-d65d-4663-82cd-87129e77c80b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_dcebb986-d65d-4663-82cd-87129e77c80b_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_dcebb986-d65d-4663-82cd-87129e77c80b" xlink:to="loc_srt_RangeMember_dcebb986-d65d-4663-82cd-87129e77c80b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_3946dbee-4f0e-47f6-abe6-3a04352c4a3e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_dcebb986-d65d-4663-82cd-87129e77c80b" xlink:to="loc_srt_RangeMember_3946dbee-4f0e-47f6-abe6-3a04352c4a3e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_f9da3791-d1ed-4c97-9919-449dc8f9a17a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_3946dbee-4f0e-47f6-abe6-3a04352c4a3e" xlink:to="loc_srt_MinimumMember_f9da3791-d1ed-4c97-9919-449dc8f9a17a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_e610a4d1-63a9-4ae4-aae6-c566379906f6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_3946dbee-4f0e-47f6-abe6-3a04352c4a3e" xlink:to="loc_srt_MaximumMember_e610a4d1-63a9-4ae4-aae6-c566379906f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_df8bc427-e90f-4880-8dc2-45ec3b9c8942" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_FiniteLivedIntangibleAssetUsefulLifeTable_9070043b-bbf0-4447-ad1d-c643fddd8640" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_df8bc427-e90f-4880-8dc2-45ec3b9c8942" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_df8bc427-e90f-4880-8dc2-45ec3b9c8942_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_df8bc427-e90f-4880-8dc2-45ec3b9c8942" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_df8bc427-e90f-4880-8dc2-45ec3b9c8942_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_d2a0c267-d259-4936-90f0-70b25feceeea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_df8bc427-e90f-4880-8dc2-45ec3b9c8942" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_d2a0c267-d259-4936-90f0-70b25feceeea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_CustomerListsAndRelationshipsMember_82606a45-48b7-4bbd-9866-8a7393121610" xlink:href="xpo-20201231.xsd#xpo_CustomerListsAndRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_d2a0c267-d259-4936-90f0-70b25feceeea" xlink:to="loc_xpo_CustomerListsAndRelationshipsMember_82606a45-48b7-4bbd-9866-8a7393121610" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccruedExpensesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesAccruedExpensesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccruedExpensesDetails" xlink:type="extended" id="if47c41d72139403fb9bcdc0b8cab7ffb_BasisofPresentationandSignificantAccountingPoliciesAccruedExpensesDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccumulatedOtherComprehensiveIncomeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesAccumulatedOtherComprehensiveIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesAccumulatedOtherComprehensiveIncomeDetails" xlink:type="extended" id="i05eaf043e47241d88734d57b2ccddfb7_BasisofPresentationandSignificantAccountingPoliciesAccumulatedOtherComprehensiveIncomeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_fb58f38e-3426-49a5-928d-0c74dd30abda" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_fb58f38e-3426-49a5-928d-0c74dd30abda" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_b1f2eb69-3579-453a-b622-a71459bc3050" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_b1f2eb69-3579-453a-b622-a71459bc3050" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_0a6be77b-6611-4467-8f4a-7bf3b6514d42" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_0a6be77b-6611-4467-8f4a-7bf3b6514d42" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_76909af7-1141-48c1-95ed-979fc585eb1e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_76909af7-1141-48c1-95ed-979fc585eb1e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_57fcb893-02fa-419c-abf1-cfde9acf76f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_1dd2a2a3-d922-43ce-b22c-838fc2dacc26" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_57fcb893-02fa-419c-abf1-cfde9acf76f1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_978c492b-3e36-4143-9444-e66dc2d3da64" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_8faec049-04ec-4429-9469-bb0e60c1004c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_fb58f38e-3426-49a5-928d-0c74dd30abda" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_8faec049-04ec-4429-9469-bb0e60c1004c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_6428b4ae-ecc1-47de-9693-0579cb3c2bdc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_8faec049-04ec-4429-9469-bb0e60c1004c" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_6428b4ae-ecc1-47de-9693-0579cb3c2bdc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6428b4ae-ecc1-47de-9693-0579cb3c2bdc_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6428b4ae-ecc1-47de-9693-0579cb3c2bdc" xlink:to="loc_us-gaap_EquityComponentDomain_6428b4ae-ecc1-47de-9693-0579cb3c2bdc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6428b4ae-ecc1-47de-9693-0579cb3c2bdc" xlink:to="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedForeignCurrencyAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember_2ecb29c2-edeb-42a2-ace4-c375b353f858" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedForeignCurrencyAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:to="loc_us-gaap_AccumulatedForeignCurrencyAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember_2ecb29c2-edeb-42a2-ace4-c375b353f858" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetGainLossFromCashFlowHedgesIncludingPortionAttributableToNoncontrollingInterestMember_d767e098-3bf0-4373-8480-1aed5c0dbec6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedNetGainLossFromCashFlowHedgesIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:to="loc_us-gaap_AccumulatedNetGainLossFromCashFlowHedgesIncludingPortionAttributableToNoncontrollingInterestMember_d767e098-3bf0-4373-8480-1aed5c0dbec6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetGainLossIncludingPortionAttributableToNoncontrollingInterestMember_114829c1-517e-40e9-be15-76be98f24745" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetGainLossIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetGainLossIncludingPortionAttributableToNoncontrollingInterestMember_114829c1-517e-40e9-be15-76be98f24745" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AociAttributableToNoncontrollingInterestMember_7126ff11-e4cf-4015-8855-e25df9c1763a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AociAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:to="loc_us-gaap_AociAttributableToNoncontrollingInterestMember_7126ff11-e4cf-4015-8855-e25df9c1763a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_3afad577-5ac4-4945-a1c6-c80183e4f3bc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_9a37571d-7a7e-4f28-ad95-817c3fd1511f" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_3afad577-5ac4-4945-a1c6-c80183e4f3bc" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesForeignCurrencyTranslationandTransactionsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesForeignCurrencyTranslationandTransactionsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesForeignCurrencyTranslationandTransactionsDetails" xlink:type="extended" id="i4130f79d584242b3bca5145f6dd3730b_BasisofPresentationandSignificantAccountingPoliciesForeignCurrencyTranslationandTransactionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_d3ae9df5-ea6c-41a4-9208-90e322386a98" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCurrencyTransactionGainLossBeforeTax_eaade2ea-59f5-4e88-bf4f-e2bc6593d1fc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignCurrencyTransactionGainLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_d3ae9df5-ea6c-41a4-9208-90e322386a98" xlink:to="loc_us-gaap_ForeignCurrencyTransactionGainLossBeforeTax_eaade2ea-59f5-4e88-bf4f-e2bc6593d1fc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_d58d3ba4-2fa8-40a1-b03d-3d83a1eb7fb4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_d3ae9df5-ea6c-41a4-9208-90e322386a98" xlink:to="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_d58d3ba4-2fa8-40a1-b03d-3d83a1eb7fb4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ForeignCurrencyTransactionAxis_98ff4934-e9b3-42f4-8c91-d169b47630ab" xlink:href="xpo-20201231.xsd#xpo_ForeignCurrencyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_d58d3ba4-2fa8-40a1-b03d-3d83a1eb7fb4" xlink:to="loc_xpo_ForeignCurrencyTransactionAxis_98ff4934-e9b3-42f4-8c91-d169b47630ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ForeignCurrencyTransactionDomain_98ff4934-e9b3-42f4-8c91-d169b47630ab_default" xlink:href="xpo-20201231.xsd#xpo_ForeignCurrencyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_xpo_ForeignCurrencyTransactionAxis_98ff4934-e9b3-42f4-8c91-d169b47630ab" xlink:to="loc_xpo_ForeignCurrencyTransactionDomain_98ff4934-e9b3-42f4-8c91-d169b47630ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ForeignCurrencyTransactionDomain_3f436025-2eac-4cbc-b75c-862948445e48" xlink:href="xpo-20201231.xsd#xpo_ForeignCurrencyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_xpo_ForeignCurrencyTransactionAxis_98ff4934-e9b3-42f4-8c91-d169b47630ab" xlink:to="loc_xpo_ForeignCurrencyTransactionDomain_3f436025-2eac-4cbc-b75c-862948445e48" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_UnrealizedGainsAndLossesOnDerivativesMember_93d87f72-173d-4537-85aa-483e960fd58c" xlink:href="xpo-20201231.xsd#xpo_UnrealizedGainsAndLossesOnDerivativesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_ForeignCurrencyTransactionDomain_3f436025-2eac-4cbc-b75c-862948445e48" xlink:to="loc_xpo_UnrealizedGainsAndLossesOnDerivativesMember_93d87f72-173d-4537-85aa-483e960fd58c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_RealizedGainsAndLossesOnDerivativeContractsMember_7d1fefcf-c230-4b40-b92a-3ae5825ceaae" xlink:href="xpo-20201231.xsd#xpo_RealizedGainsAndLossesOnDerivativeContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_ForeignCurrencyTransactionDomain_3f436025-2eac-4cbc-b75c-862948445e48" xlink:to="loc_xpo_RealizedGainsAndLossesOnDerivativeContractsMember_7d1fefcf-c230-4b40-b92a-3ae5825ceaae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ForeignCurrencyTransactionAndRemeasurementGainsAndLossesMember_f1062b11-2d05-4b94-9356-83eecc45b2a1" xlink:href="xpo-20201231.xsd#xpo_ForeignCurrencyTransactionAndRemeasurementGainsAndLossesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_ForeignCurrencyTransactionDomain_3f436025-2eac-4cbc-b75c-862948445e48" xlink:to="loc_xpo_ForeignCurrencyTransactionAndRemeasurementGainsAndLossesMember_f1062b11-2d05-4b94-9356-83eecc45b2a1" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesFairValueMeasurementsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#BasisofPresentationandSignificantAccountingPoliciesFairValueMeasurementsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/BasisofPresentationandSignificantAccountingPoliciesFairValueMeasurementsDetails" xlink:type="extended" id="ic191392e9903422eb3b2e8dd117b97e1_BasisofPresentationandSignificantAccountingPoliciesFairValueMeasurementsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5ec41e4f-3620-4ba8-a301-46f17579dad4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_28a1fc4c-b0b8-4554-bd5e-10fcaa0c9f19" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5ec41e4f-3620-4ba8-a301-46f17579dad4" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_28a1fc4c-b0b8-4554-bd5e-10fcaa0c9f19" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_cc11e2a0-6f70-4001-90f4-509f1f6dd39f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5ec41e4f-3620-4ba8-a301-46f17579dad4" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_cc11e2a0-6f70-4001-90f4-509f1f6dd39f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_db960423-6ccb-47ce-879b-d67d1a915146" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_cc11e2a0-6f70-4001-90f4-509f1f6dd39f" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_db960423-6ccb-47ce-879b-d67d1a915146" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_db960423-6ccb-47ce-879b-d67d1a915146_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_db960423-6ccb-47ce-879b-d67d1a915146" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_db960423-6ccb-47ce-879b-d67d1a915146_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3c6d7c3c-a318-484a-8b6c-8fc3869ff8b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_db960423-6ccb-47ce-879b-d67d1a915146" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3c6d7c3c-a318-484a-8b6c-8fc3869ff8b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c1ac5ff4-b3e1-4600-b97d-337b4a8e86b0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3c6d7c3c-a318-484a-8b6c-8fc3869ff8b6" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c1ac5ff4-b3e1-4600-b97d-337b4a8e86b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_194a4879-c9f3-4f68-a95a-e52678a6ce32" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c1ac5ff4-b3e1-4600-b97d-337b4a8e86b0" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_194a4879-c9f3-4f68-a95a-e52678a6ce32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_cc11e2a0-6f70-4001-90f4-509f1f6dd39f" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_862435fb-2dad-4d5e-8c8e-7c170b5be6d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_a6b6b0fb-8987-49cc-9bb6-aaa97313a9e9" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_862435fb-2dad-4d5e-8c8e-7c170b5be6d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_c068ec58-99e8-458f-b742-9d5751232bf9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_862435fb-2dad-4d5e-8c8e-7c170b5be6d6" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_c068ec58-99e8-458f-b742-9d5751232bf9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_74848e5d-40bf-4645-b8f9-29f297063278" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_862435fb-2dad-4d5e-8c8e-7c170b5be6d6" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_74848e5d-40bf-4645-b8f9-29f297063278" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/PurchaseofNoncontrollingInterest" xlink:type="simple" xlink:href="xpo-20201231.xsd#PurchaseofNoncontrollingInterest"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PurchaseofNoncontrollingInterest" xlink:type="extended" id="i919e227b91a643f086942ff12ca21969_PurchaseofNoncontrollingInterest"/>
  <link:roleRef roleURI="http://www.xpo.com/role/PurchaseofNoncontrollingInterestDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#PurchaseofNoncontrollingInterestDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PurchaseofNoncontrollingInterestDetails" xlink:type="extended" id="ia3ae95266ea244dea1b8bc3c363dca90_PurchaseofNoncontrollingInterestDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/SegmentReportingandGeographicInformation" xlink:type="simple" xlink:href="xpo-20201231.xsd#SegmentReportingandGeographicInformation"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SegmentReportingandGeographicInformation" xlink:type="extended" id="i6a742cd0572c42ec9b235ab5167fc233_SegmentReportingandGeographicInformation"/>
  <link:roleRef roleURI="http://www.xpo.com/role/SegmentReportingandGeographicInformationTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#SegmentReportingandGeographicInformationTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SegmentReportingandGeographicInformationTables" xlink:type="extended" id="i1070d17ef2dc4762b233d6297ec5845c_SegmentReportingandGeographicInformationTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/SegmentReportingandGeographicInformationNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#SegmentReportingandGeographicInformationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SegmentReportingandGeographicInformationNarrativeDetails" xlink:type="extended" id="i4e3fd0d5d61f4e6087d3cfb6e2521759_SegmentReportingandGeographicInformationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_98bb8d81-6185-414a-abc7-9ca3d718ab18" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_afadf0dd-b3ba-4e88-8638-e972132e076f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_98bb8d81-6185-414a-abc7-9ca3d718ab18" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_afadf0dd-b3ba-4e88-8638-e972132e076f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_8c44f0dc-db69-43a3-93d9-4b61f58760d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenuesFromExternalCustomersAndLongLivedAssetsLineItems_98bb8d81-6185-414a-abc7-9ca3d718ab18" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_8c44f0dc-db69-43a3-93d9-4b61f58760d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_a12e6685-5cd2-4014-ac72-52b2ccf2a992" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_8c44f0dc-db69-43a3-93d9-4b61f58760d7" xlink:to="loc_srt_StatementGeographicalAxis_a12e6685-5cd2-4014-ac72-52b2ccf2a992" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_a12e6685-5cd2-4014-ac72-52b2ccf2a992_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_a12e6685-5cd2-4014-ac72-52b2ccf2a992" xlink:to="loc_srt_SegmentGeographicalDomain_a12e6685-5cd2-4014-ac72-52b2ccf2a992_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_af0adf03-8ed9-4ba7-82a0-f997b5e87667" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_a12e6685-5cd2-4014-ac72-52b2ccf2a992" xlink:to="loc_srt_SegmentGeographicalDomain_af0adf03-8ed9-4ba7-82a0-f997b5e87667" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_8f86bd92-8a04-4c50-9d19-6201c12e33b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_af0adf03-8ed9-4ba7-82a0-f997b5e87667" xlink:to="loc_us-gaap_NonUsMember_8f86bd92-8a04-4c50-9d19-6201c12e33b6" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/SegmentReportingandGeographicInformationSelectedFinancialDataforEachReportableSegmentDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#SegmentReportingandGeographicInformationSelectedFinancialDataforEachReportableSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SegmentReportingandGeographicInformationSelectedFinancialDataforEachReportableSegmentDetails" xlink:type="extended" id="ib115338f0b0c4062ae07f281563b22e5_SegmentReportingandGeographicInformationSelectedFinancialDataforEachReportableSegmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_4ca01e85-e141-47bc-855f-15287e86afc3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_Revenues_4ca01e85-e141-47bc-855f-15287e86afc3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_062b0302-8435-4bf0-97ac-f9d9e466b686" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_OperatingIncomeLoss_062b0302-8435-4bf0-97ac-f9d9e466b686" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_33f553dd-3f6c-4354-a73f-714822b15dc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_DepreciationAndAmortization_33f553dd-3f6c-4354-a73f-714822b15dc6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransactionAndIntegrationCosts_3bad8325-2627-46d3-9d22-8dc0729344e6" xlink:href="xpo-20201231.xsd#xpo_TransactionAndIntegrationCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_xpo_TransactionAndIntegrationCosts_3bad8325-2627-46d3-9d22-8dc0729344e6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCosts_9f87e8b2-b717-44b6-a8d7-48e3424927c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_RestructuringCosts_9f87e8b2-b717-44b6-a8d7-48e3424927c3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransactionIntegrationCostsAndRebrandingCosts_27470491-6a48-4e0b-ac40-b89d805b2b2c" xlink:href="xpo-20201231.xsd#xpo_TransactionIntegrationCostsAndRebrandingCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_xpo_TransactionIntegrationCostsAndRebrandingCosts_27470491-6a48-4e0b-ac40-b89d805b2b2c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationSettlementExpense_822f0fd6-709b-4079-a52e-839ac17206ad" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LitigationSettlementExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_LitigationSettlementExpense_822f0fd6-709b-4079-a52e-839ac17206ad" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentRealizedGainLossOnDisposal_6fd8582b-d9b0-45be-819a-32b8a35e58c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityMethodInvestmentRealizedGainLossOnDisposal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_EquityMethodInvestmentRealizedGainLossOnDisposal_6fd8582b-d9b0-45be-819a-32b8a35e58c2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_2fd5a817-e32b-4ddb-bb72-0022d0e77ad5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_4de85aa3-bc6e-421a-869b-546cfc890e7a" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_2fd5a817-e32b-4ddb-bb72-0022d0e77ad5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_72a323af-a071-414d-8c2e-f36c0ce6dd5f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_2fd5a817-e32b-4ddb-bb72-0022d0e77ad5" xlink:to="loc_srt_ConsolidationItemsAxis_72a323af-a071-414d-8c2e-f36c0ce6dd5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_72a323af-a071-414d-8c2e-f36c0ce6dd5f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_72a323af-a071-414d-8c2e-f36c0ce6dd5f" xlink:to="loc_srt_ConsolidationItemsDomain_72a323af-a071-414d-8c2e-f36c0ce6dd5f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_4dffc120-6cc8-4211-bb26-cc68f2d59fbb" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_72a323af-a071-414d-8c2e-f36c0ce6dd5f" xlink:to="loc_srt_ConsolidationItemsDomain_4dffc120-6cc8-4211-bb26-cc68f2d59fbb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_5a32c95c-3e39-4e32-ba5d-2b2922e3234c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_4dffc120-6cc8-4211-bb26-cc68f2d59fbb" xlink:to="loc_us-gaap_OperatingSegmentsMember_5a32c95c-3e39-4e32-ba5d-2b2922e3234c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_2be7710e-1719-46f5-b49c-81d26474f40d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_4dffc120-6cc8-4211-bb26-cc68f2d59fbb" xlink:to="loc_us-gaap_CorporateNonSegmentMember_2be7710e-1719-46f5-b49c-81d26474f40d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_87e10fb4-a8c5-4d8b-b93d-2b32b1918f5f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_4dffc120-6cc8-4211-bb26-cc68f2d59fbb" xlink:to="loc_us-gaap_IntersegmentEliminationMember_87e10fb4-a8c5-4d8b-b93d-2b32b1918f5f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_2fd5a817-e32b-4ddb-bb72-0022d0e77ad5" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c" xlink:to="loc_us-gaap_SegmentDomain_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_b7884def-0c1b-48a3-9720-c545e3c62b20" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_b6b8c3db-a8e3-41cc-8b7f-20f8d07baa5c" xlink:to="loc_us-gaap_SegmentDomain_b7884def-0c1b-48a3-9720-c545e3c62b20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ExpeditedTransportationMember_33c0e38d-c99e-4aa4-a465-42d02c9e9ac7" xlink:href="xpo-20201231.xsd#xpo_ExpeditedTransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b7884def-0c1b-48a3-9720-c545e3c62b20" xlink:to="loc_xpo_ExpeditedTransportationMember_33c0e38d-c99e-4aa4-a465-42d02c9e9ac7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ContractLogisticsMember_bf4662f5-ad58-4ce4-82b1-12a15820083c" xlink:href="xpo-20201231.xsd#xpo_ContractLogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b7884def-0c1b-48a3-9720-c545e3c62b20" xlink:to="loc_xpo_ContractLogisticsMember_bf4662f5-ad58-4ce4-82b1-12a15820083c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognition" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognition"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognition" xlink:type="extended" id="ide24913cc826449a8d4b6b2c8ff62ec4_RevenueRecognition"/>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognitionTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognitionTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognitionTables" xlink:type="extended" id="id9b6d8f6ddbe4ff4bcc3e019a60914da_RevenueRecognitionTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognitionDisaggregationofRevenuebyGeographicalAreaDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognitionDisaggregationofRevenuebyGeographicalAreaDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognitionDisaggregationofRevenuebyGeographicalAreaDetails" xlink:type="extended" id="ic5c9f3641a9a411480c4471d70d43a54_RevenueRecognitionDisaggregationofRevenuebyGeographicalAreaDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_a75a32d0-9ad9-4646-817b-ac988c8367ea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c50fec5e-00f3-47cc-90a4-71dc0f918982" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_a75a32d0-9ad9-4646-817b-ac988c8367ea" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c50fec5e-00f3-47cc-90a4-71dc0f918982" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_9afadb8e-f0d6-49f2-b147-9a79aa605762" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_a75a32d0-9ad9-4646-817b-ac988c8367ea" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_9afadb8e-f0d6-49f2-b147-9a79aa605762" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_0258884a-8a3f-41fb-b08f-06d772ecb805" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_9afadb8e-f0d6-49f2-b147-9a79aa605762" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_0258884a-8a3f-41fb-b08f-06d772ecb805" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_0258884a-8a3f-41fb-b08f-06d772ecb805_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_0258884a-8a3f-41fb-b08f-06d772ecb805" xlink:to="loc_us-gaap_SegmentDomain_0258884a-8a3f-41fb-b08f-06d772ecb805_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_8a093ddb-5af9-4eee-adfc-5ec5c7c7587d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_0258884a-8a3f-41fb-b08f-06d772ecb805" xlink:to="loc_us-gaap_SegmentDomain_8a093ddb-5af9-4eee-adfc-5ec5c7c7587d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransportationMember_a795c8b5-7112-418d-84e7-c63310a18d9f" xlink:href="xpo-20201231.xsd#xpo_TransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_8a093ddb-5af9-4eee-adfc-5ec5c7c7587d" xlink:to="loc_xpo_TransportationMember_a795c8b5-7112-418d-84e7-c63310a18d9f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ContractLogisticsMember_7936c74f-d2c6-4143-96f4-e06c51926ba6" xlink:href="xpo-20201231.xsd#xpo_ContractLogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_8a093ddb-5af9-4eee-adfc-5ec5c7c7587d" xlink:to="loc_xpo_ContractLogisticsMember_7936c74f-d2c6-4143-96f4-e06c51926ba6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_e3ceca64-78a6-416b-ae2b-1fed990a2a6e" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_9afadb8e-f0d6-49f2-b147-9a79aa605762" xlink:to="loc_srt_ConsolidationItemsAxis_e3ceca64-78a6-416b-ae2b-1fed990a2a6e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_e3ceca64-78a6-416b-ae2b-1fed990a2a6e_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_e3ceca64-78a6-416b-ae2b-1fed990a2a6e" xlink:to="loc_srt_ConsolidationItemsDomain_e3ceca64-78a6-416b-ae2b-1fed990a2a6e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_7b4a80cf-d4e0-4ad8-8c27-f8b68ec8c193" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_e3ceca64-78a6-416b-ae2b-1fed990a2a6e" xlink:to="loc_srt_ConsolidationItemsDomain_7b4a80cf-d4e0-4ad8-8c27-f8b68ec8c193" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_56eba95f-5b78-46b9-add9-d68ce98d8f23" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_7b4a80cf-d4e0-4ad8-8c27-f8b68ec8c193" xlink:to="loc_us-gaap_OperatingSegmentsMember_56eba95f-5b78-46b9-add9-d68ce98d8f23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_c2c5f7be-5ae3-4d40-9d89-06e344766d73" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_7b4a80cf-d4e0-4ad8-8c27-f8b68ec8c193" xlink:to="loc_us-gaap_IntersegmentEliminationMember_c2c5f7be-5ae3-4d40-9d89-06e344766d73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_1cab8fee-098f-40a3-9eb9-f705d326061f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_9afadb8e-f0d6-49f2-b147-9a79aa605762" xlink:to="loc_srt_StatementGeographicalAxis_1cab8fee-098f-40a3-9eb9-f705d326061f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_1cab8fee-098f-40a3-9eb9-f705d326061f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_1cab8fee-098f-40a3-9eb9-f705d326061f" xlink:to="loc_srt_SegmentGeographicalDomain_1cab8fee-098f-40a3-9eb9-f705d326061f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_1cab8fee-098f-40a3-9eb9-f705d326061f" xlink:to="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_e6067887-b716-4688-a426-a81a037a2f59" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_country_US_e6067887-b716-4688-a426-a81a037a2f59" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_NorthAmericaExcludingUnitedStatesMember_cdddd792-02bb-4475-bc6a-809cd147fa19" xlink:href="xpo-20201231.xsd#xpo_NorthAmericaExcludingUnitedStatesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_xpo_NorthAmericaExcludingUnitedStatesMember_cdddd792-02bb-4475-bc6a-809cd147fa19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_FR_dbd0f265-14a8-4dfd-b696-6c9e124a7a1e" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_FR"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_country_FR_dbd0f265-14a8-4dfd-b696-6c9e124a7a1e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_GB_3fc9374b-5fd4-48c4-8852-c14a00753696" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_GB"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_country_GB_3fc9374b-5fd4-48c4-8852-c14a00753696" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EuropeExcludingFranceAndUnitedKingdomMember_e77bca24-1e33-40ad-b7ad-9349ca4bb354" xlink:href="xpo-20201231.xsd#xpo_EuropeExcludingFranceAndUnitedKingdomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_xpo_EuropeExcludingFranceAndUnitedKingdomMember_e77bca24-1e33-40ad-b7ad-9349ca4bb354" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_OtherGeographicalAreasMember_02fcc0c8-b79c-42a2-8aa4-6521513839c8" xlink:href="xpo-20201231.xsd#xpo_OtherGeographicalAreasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_67c9985b-e1dd-4f8a-af16-51e91e70a017" xlink:to="loc_xpo_OtherGeographicalAreasMember_02fcc0c8-b79c-42a2-8aa4-6521513839c8" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognitionDisaggregationofRevenuebyProductsandServicesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognitionDisaggregationofRevenuebyProductsandServicesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognitionDisaggregationofRevenuebyProductsandServicesDetails" xlink:type="extended" id="i9ca6164901db4164b734d98ead4ec051_RevenueRecognitionDisaggregationofRevenuebyProductsandServicesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_5dd278d9-5f52-4024-8447-28c9dbde4bf3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_831e5dec-c0ea-467b-a54a-571301521015" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_5dd278d9-5f52-4024-8447-28c9dbde4bf3" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_831e5dec-c0ea-467b-a54a-571301521015" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_69c71f29-30d9-4a0d-80d9-ea81ff75ad6c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_5dd278d9-5f52-4024-8447-28c9dbde4bf3" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_69c71f29-30d9-4a0d-80d9-ea81ff75ad6c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_2b1702c2-dd42-4fe2-a760-6e86cdfee9de" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69c71f29-30d9-4a0d-80d9-ea81ff75ad6c" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_2b1702c2-dd42-4fe2-a760-6e86cdfee9de" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_2b1702c2-dd42-4fe2-a760-6e86cdfee9de_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_2b1702c2-dd42-4fe2-a760-6e86cdfee9de" xlink:to="loc_us-gaap_SegmentDomain_2b1702c2-dd42-4fe2-a760-6e86cdfee9de_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_f1872b08-9795-41a4-886f-573ba1d9939a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_2b1702c2-dd42-4fe2-a760-6e86cdfee9de" xlink:to="loc_us-gaap_SegmentDomain_f1872b08-9795-41a4-886f-573ba1d9939a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransportationMember_f8bba5dc-6dc5-41df-8263-b57d72aaf818" xlink:href="xpo-20201231.xsd#xpo_TransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_f1872b08-9795-41a4-886f-573ba1d9939a" xlink:to="loc_xpo_TransportationMember_f8bba5dc-6dc5-41df-8263-b57d72aaf818" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ContractLogisticsMember_cbab2723-54e8-4f58-89a2-1d7d7be4c232" xlink:href="xpo-20201231.xsd#xpo_ContractLogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_f1872b08-9795-41a4-886f-573ba1d9939a" xlink:to="loc_xpo_ContractLogisticsMember_cbab2723-54e8-4f58-89a2-1d7d7be4c232" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_623162f3-2cfc-47c1-b4db-dd54be869ec1" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69c71f29-30d9-4a0d-80d9-ea81ff75ad6c" xlink:to="loc_srt_ConsolidationItemsAxis_623162f3-2cfc-47c1-b4db-dd54be869ec1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_623162f3-2cfc-47c1-b4db-dd54be869ec1_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_623162f3-2cfc-47c1-b4db-dd54be869ec1" xlink:to="loc_srt_ConsolidationItemsDomain_623162f3-2cfc-47c1-b4db-dd54be869ec1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_9161aff7-5b11-420e-9967-d51dd5057a84" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_623162f3-2cfc-47c1-b4db-dd54be869ec1" xlink:to="loc_srt_ConsolidationItemsDomain_9161aff7-5b11-420e-9967-d51dd5057a84" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_155083b1-6995-4a13-9e2e-04f4dc972f5c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_9161aff7-5b11-420e-9967-d51dd5057a84" xlink:to="loc_us-gaap_OperatingSegmentsMember_155083b1-6995-4a13-9e2e-04f4dc972f5c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_51a0ac8a-9c86-4255-a877-753d3a27805f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_9161aff7-5b11-420e-9967-d51dd5057a84" xlink:to="loc_us-gaap_IntersegmentEliminationMember_51a0ac8a-9c86-4255-a877-753d3a27805f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_3282cef9-14c0-49c1-8dde-304ce3516729" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69c71f29-30d9-4a0d-80d9-ea81ff75ad6c" xlink:to="loc_srt_ProductOrServiceAxis_3282cef9-14c0-49c1-8dde-304ce3516729" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_3282cef9-14c0-49c1-8dde-304ce3516729_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_3282cef9-14c0-49c1-8dde-304ce3516729" xlink:to="loc_srt_ProductsAndServicesDomain_3282cef9-14c0-49c1-8dde-304ce3516729_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_3282cef9-14c0-49c1-8dde-304ce3516729" xlink:to="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LessThanTruckloadMember_e30269e8-b92b-44ca-a43b-03bfc667f3c3" xlink:href="xpo-20201231.xsd#xpo_LessThanTruckloadMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:to="loc_xpo_LessThanTruckloadMember_e30269e8-b92b-44ca-a43b-03bfc667f3c3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_FreightBrokerageAndTruckloadMember_d2a91826-cc88-4671-8315-a13782c95984" xlink:href="xpo-20201231.xsd#xpo_FreightBrokerageAndTruckloadMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:to="loc_xpo_FreightBrokerageAndTruckloadMember_d2a91826-cc88-4671-8315-a13782c95984" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LastMileMember_7b6e8d37-6b98-4744-8f68-dfcdea2c475c" xlink:href="xpo-20201231.xsd#xpo_LastMileMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:to="loc_xpo_LastMileMember_7b6e8d37-6b98-4744-8f68-dfcdea2c475c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ManagedTransportationMember_e391d0eb-aae1-4fe6-8cbc-dff590cfae3a" xlink:href="xpo-20201231.xsd#xpo_ManagedTransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:to="loc_xpo_ManagedTransportationMember_e391d0eb-aae1-4fe6-8cbc-dff590cfae3a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_GlobalForwardingMember_779839af-08a9-4e38-bbb7-f6b38ecf6c34" xlink:href="xpo-20201231.xsd#xpo_GlobalForwardingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_0144a4bf-015a-4a22-88d7-51c8ea7d0ec2" xlink:to="loc_xpo_GlobalForwardingMember_779839af-08a9-4e38-bbb7-f6b38ecf6c34" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognitionPerformanceObligationsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognitionPerformanceObligationsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognitionPerformanceObligationsDetails" xlink:type="extended" id="i5e806072d1eb4f87858a98dbbb1d3bff_RevenueRecognitionPerformanceObligationsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_2ad3ae8d-2919-4151-9050-5665bec0f2b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_2ad3ae8d-2919-4151-9050-5665bec0f2b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_9dc6a661-9695-4274-ac8e-43204f39534f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_9dc6a661-9695-4274-ac8e-43204f39534f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_7a421a0a-8b2f-4f84-ab50-0b68305d0741" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_7a421a0a-8b2f-4f84-ab50-0b68305d0741" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_d40df12d-2752-479e-8a5b-33a2b44a927e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_d40df12d-2752-479e-8a5b-33a2b44a927e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_f712a7d3-365a-4010-b480-32c17a30775e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_d40df12d-2752-479e-8a5b-33a2b44a927e" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionStartDateAxis_f712a7d3-365a-4010-b480-32c17a30775e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RevenueRecognitionPerformanceObligationsDetails_1" xlink:type="simple" xlink:href="xpo-20201231.xsd#RevenueRecognitionPerformanceObligationsDetails_1"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RevenueRecognitionPerformanceObligationsDetails_1" xlink:type="extended" id="i164cc05263544eae90499d2b95198c83_RevenueRecognitionPerformanceObligationsDetails_1">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_2ad3ae8d-2919-4151-9050-5665bec0f2b8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_2ad3ae8d-2919-4151-9050-5665bec0f2b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_9dc6a661-9695-4274-ac8e-43204f39534f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_9dc6a661-9695-4274-ac8e-43204f39534f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_7a421a0a-8b2f-4f84-ab50-0b68305d0741" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionPeriod1_7a421a0a-8b2f-4f84-ab50-0b68305d0741" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_d40df12d-2752-479e-8a5b-33a2b44a927e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_daab4882-6de4-4a48-954e-7ad2764ddcc6" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_d40df12d-2752-479e-8a5b-33a2b44a927e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RestructuringCharges" xlink:type="simple" xlink:href="xpo-20201231.xsd#RestructuringCharges"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RestructuringCharges" xlink:type="extended" id="ifc78b006524447099eabdf8965870aef_RestructuringCharges"/>
  <link:roleRef roleURI="http://www.xpo.com/role/RestructuringChargesTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#RestructuringChargesTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RestructuringChargesTables" xlink:type="extended" id="i486a1e48fd4d40d0a6528d49d50af303_RestructuringChargesTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/RestructuringChargesScheduleofRestructuringChargesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RestructuringChargesScheduleofRestructuringChargesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RestructuringChargesScheduleofRestructuringChargesDetails" xlink:type="extended" id="i3f274351578e4ca48e38dafea3747fc9_RestructuringChargesScheduleofRestructuringChargesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_f9168d62-c659-4c20-b8b6-0552c1bc960e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCharges_85d8ee18-8cab-407e-92f7-dc8b08b36a4c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_f9168d62-c659-4c20-b8b6-0552c1bc960e" xlink:to="loc_us-gaap_RestructuringCharges_85d8ee18-8cab-407e-92f7-dc8b08b36a4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_ff6e1780-e4ce-4c71-9029-8905a3af3f36" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_f9168d62-c659-4c20-b8b6-0552c1bc960e" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_ff6e1780-e4ce-4c71-9029-8905a3af3f36" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_eb7fa1ef-4928-4944-ac1f-8d2b122359c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_ff6e1780-e4ce-4c71-9029-8905a3af3f36" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_eb7fa1ef-4928-4944-ac1f-8d2b122359c4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_eb7fa1ef-4928-4944-ac1f-8d2b122359c4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_eb7fa1ef-4928-4944-ac1f-8d2b122359c4" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_eb7fa1ef-4928-4944-ac1f-8d2b122359c4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_d8e34e54-41ba-4c6a-b4c9-da0c224678aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_eb7fa1ef-4928-4944-ac1f-8d2b122359c4" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_d8e34e54-41ba-4c6a-b4c9-da0c224678aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_8c7f78ed-d431-4c37-8676-d3ce64707be1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d8e34e54-41ba-4c6a-b4c9-da0c224678aa" xlink:to="loc_us-gaap_CostOfSalesMember_8c7f78ed-d431-4c37-8676-d3ce64707be1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenseMember_c82a9be8-26de-413c-a10c-afd2a5b153b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d8e34e54-41ba-4c6a-b4c9-da0c224678aa" xlink:to="loc_us-gaap_OperatingExpenseMember_c82a9be8-26de-413c-a10c-afd2a5b153b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_351474d1-8dae-4cd3-ad0f-927ab9383240" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingGeneralAndAdministrativeExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d8e34e54-41ba-4c6a-b4c9-da0c224678aa" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_351474d1-8dae-4cd3-ad0f-927ab9383240" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/RestructuringChargesNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RestructuringChargesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RestructuringChargesNarrativeDetails" xlink:type="extended" id="if1e68196ae274aef8e70660768def742_RestructuringChargesNarrativeDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/RestructuringChargesScheduleofRestructuringRelatedActivityDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#RestructuringChargesScheduleofRestructuringRelatedActivityDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/RestructuringChargesScheduleofRestructuringRelatedActivityDetails" xlink:type="extended" id="i7643c7979eda4bba962588ac31cd75be_RestructuringChargesScheduleofRestructuringRelatedActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_80c1a3c6-44d6-4d85-93b0-435611a9715d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringReserveRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_80c1a3c6-44d6-4d85-93b0-435611a9715d" xlink:to="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringReserve_35b97d37-599d-4c6c-80d6-8c60d31d851c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringReserve"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:to="loc_us-gaap_RestructuringReserve_35b97d37-599d-4c6c-80d6-8c60d31d851c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCharges_483d0d6a-5a5b-4c61-8c52-1ea8f9718905" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:to="loc_us-gaap_RestructuringCharges_483d0d6a-5a5b-4c61-8c52-1ea8f9718905" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRestructuring_63d62572-cfc6-432b-b35e-e5b14b93def4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsForRestructuring"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:to="loc_us-gaap_PaymentsForRestructuring_63d62572-cfc6-432b-b35e-e5b14b93def4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringReserveTranslationAdjustment_234c80d7-67e1-4113-ba70-2e2767c17d68" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringReserveTranslationAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringReserveRollForward_1f2121bb-ebda-4245-82c1-e91d3549f354" xlink:to="loc_us-gaap_RestructuringReserveTranslationAdjustment_234c80d7-67e1-4113-ba70-2e2767c17d68" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringReserve_d13b0f19-db2f-4235-ad19-0742eeffb8ce" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringReserve"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_b92f90f7-ed0f-4e18-a3c0-86eba70edfc0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_80c1a3c6-44d6-4d85-93b0-435611a9715d" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_b92f90f7-ed0f-4e18-a3c0-86eba70edfc0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_b92f90f7-ed0f-4e18-a3c0-86eba70edfc0" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c" xlink:to="loc_us-gaap_SegmentDomain_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_19915737-51d9-41ef-bfa6-885fd347307c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_253cf9f8-1a7e-4656-8bad-0dc1b894ef9c" xlink:to="loc_us-gaap_SegmentDomain_19915737-51d9-41ef-bfa6-885fd347307c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransportationMember_8de2dc05-15cf-4bd9-b94e-a12046a02d43" xlink:href="xpo-20201231.xsd#xpo_TransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_19915737-51d9-41ef-bfa6-885fd347307c" xlink:to="loc_xpo_TransportationMember_8de2dc05-15cf-4bd9-b94e-a12046a02d43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ContractLogisticsMember_da3250ef-238b-4e0c-8c8d-3f883dda75fa" xlink:href="xpo-20201231.xsd#xpo_ContractLogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_19915737-51d9-41ef-bfa6-885fd347307c" xlink:to="loc_xpo_ContractLogisticsMember_da3250ef-238b-4e0c-8c8d-3f883dda75fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_94095a85-d903-4a45-aa0c-f206b21acd0c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_b92f90f7-ed0f-4e18-a3c0-86eba70edfc0" xlink:to="loc_srt_ConsolidationItemsAxis_94095a85-d903-4a45-aa0c-f206b21acd0c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_94095a85-d903-4a45-aa0c-f206b21acd0c_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_94095a85-d903-4a45-aa0c-f206b21acd0c" xlink:to="loc_srt_ConsolidationItemsDomain_94095a85-d903-4a45-aa0c-f206b21acd0c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_ccfc6244-b4a3-44d1-a912-823d71985ce0" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_94095a85-d903-4a45-aa0c-f206b21acd0c" xlink:to="loc_srt_ConsolidationItemsDomain_ccfc6244-b4a3-44d1-a912-823d71985ce0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_5bc742c6-9cbe-4873-a2df-55e706f5db78" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_ccfc6244-b4a3-44d1-a912-823d71985ce0" xlink:to="loc_us-gaap_OperatingSegmentsMember_5bc742c6-9cbe-4873-a2df-55e706f5db78" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_ee657ea7-c00d-47d4-821e-597578814a7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_ccfc6244-b4a3-44d1-a912-823d71985ce0" xlink:to="loc_us-gaap_CorporateNonSegmentMember_ee657ea7-c00d-47d4-821e-597578814a7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveAxis_bf219ce4-c33b-413f-831c-5fa1ba9386b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestructuringCostAndReserveAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_b92f90f7-ed0f-4e18-a3c0-86eba70edfc0" xlink:to="loc_us-gaap_RestructuringCostAndReserveAxis_bf219ce4-c33b-413f-831c-5fa1ba9386b9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfRestructuringDomain_bf219ce4-c33b-413f-831c-5fa1ba9386b9_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TypeOfRestructuringDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RestructuringCostAndReserveAxis_bf219ce4-c33b-413f-831c-5fa1ba9386b9" xlink:to="loc_us-gaap_TypeOfRestructuringDomain_bf219ce4-c33b-413f-831c-5fa1ba9386b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfRestructuringDomain_ed4676a9-9fe1-441e-bd41-55c2c6ffe302" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TypeOfRestructuringDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RestructuringCostAndReserveAxis_bf219ce4-c33b-413f-831c-5fa1ba9386b9" xlink:to="loc_us-gaap_TypeOfRestructuringDomain_ed4676a9-9fe1-441e-bd41-55c2c6ffe302" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeSeveranceMember_7b5e4f30-b205-4300-981e-fff256b2ff8f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeSeveranceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TypeOfRestructuringDomain_ed4676a9-9fe1-441e-bd41-55c2c6ffe302" xlink:to="loc_us-gaap_EmployeeSeveranceMember_7b5e4f30-b205-4300-981e-fff256b2ff8f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FacilityClosingMember_a3a1cd70-f64c-401b-bb4d-5365dfef35f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FacilityClosingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TypeOfRestructuringDomain_ed4676a9-9fe1-441e-bd41-55c2c6ffe302" xlink:to="loc_us-gaap_FacilityClosingMember_a3a1cd70-f64c-401b-bb4d-5365dfef35f6" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/PropertyandEquipment" xlink:type="simple" xlink:href="xpo-20201231.xsd#PropertyandEquipment"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PropertyandEquipment" xlink:type="extended" id="if9ed72f035f34539bc68e9322b5b66a9_PropertyandEquipment"/>
  <link:roleRef roleURI="http://www.xpo.com/role/PropertyandEquipmentTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#PropertyandEquipmentTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PropertyandEquipmentTables" xlink:type="extended" id="i1b19d05bbe914220ba82696349fdca58_PropertyandEquipmentTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/PropertyandEquipmentSummaryofPropertyandEquipmentDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#PropertyandEquipmentSummaryofPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PropertyandEquipmentSummaryofPropertyandEquipmentDetails" xlink:type="extended" id="i8f64a2f5ffbf44d1a2617eba7fa0815f_PropertyandEquipmentSummaryofPropertyandEquipmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_7477539e-b185-47a0-8f64-a716b3100a84" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_7477539e-b185-47a0-8f64-a716b3100a84" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_4623c47b-0ba7-426b-a9e3-34c7a01b70e5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_4623c47b-0ba7-426b-a9e3-34c7a01b70e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_a0cc34c8-257e-4710-909d-28e55136ff15" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_a0cc34c8-257e-4710-909d-28e55136ff15" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedComputerSoftwareNet_1383ebcf-a5ed-4b1b-9a64-520cf02b146b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalizedComputerSoftwareNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:to="loc_us-gaap_CapitalizedComputerSoftwareNet_1383ebcf-a5ed-4b1b-9a64-520cf02b146b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_2f2651ab-e7f9-4d08-adec-8f295f15c117" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_f3eec422-efc6-4ea4-b6b4-eb9d7187e7ca" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_2f2651ab-e7f9-4d08-adec-8f295f15c117" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_2f2651ab-e7f9-4d08-adec-8f295f15c117" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_d6da9fc4-12b9-40cd-a78c-dbf5deed5e92" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LandMember_90ae5907-91b4-4974-815d-bd85bbf9d164" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:to="loc_us-gaap_LandMember_90ae5907-91b4-4974-815d-bd85bbf9d164" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_0a276168-6143-43a1-a0b1-ddb69b756fb9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_0a276168-6143-43a1-a0b1-ddb69b756fb9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VehiclesMember_c26aa4e4-6b5b-47bc-a565-9fa708cc2f7f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:to="loc_us-gaap_VehiclesMember_c26aa4e4-6b5b-47bc-a565-9fa708cc2f7f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MachineryAndEquipmentMember_52e41555-c70f-4388-9f64-9f156414868f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MachineryAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:to="loc_us-gaap_MachineryAndEquipmentMember_52e41555-c70f-4388-9f64-9f156414868f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ComputerSoftwareMember_c0ce84ae-46c2-4ed8-a295-e8568a4544ee" xlink:href="xpo-20201231.xsd#xpo_ComputerSoftwareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_af48e5c4-7db8-4bcc-87bb-ddfd10607407" xlink:to="loc_xpo_ComputerSoftwareMember_c0ce84ae-46c2-4ed8-a295-e8568a4544ee" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/PropertyandEquipmentNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#PropertyandEquipmentNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/PropertyandEquipmentNarrativeDetails" xlink:type="extended" id="i418beb6a195c4afb93635710ff04a056_PropertyandEquipmentNarrativeDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/Leases" xlink:type="simple" xlink:href="xpo-20201231.xsd#Leases"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/Leases" xlink:type="extended" id="ibd5525a1bc0946c1b64e57e69cc437af_Leases"/>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesTables" xlink:type="extended" id="id5ca80eed77c442a8cce5f0e388b10f9_LeasesTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesNarrativeDetails" xlink:type="extended" id="i112a304577624a0cbe23f10ec5bfa8fa_LeasesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalLeaseObligationsIncurred_ac75fcf0-6a56-4b45-b1ee-6a268cb2230d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CapitalLeaseObligationsIncurred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_us-gaap_CapitalLeaseObligationsIncurred_ac75fcf0-6a56-4b45-b1ee-6a268cb2230d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PropertyAndEquipmentAdditionsNonCashInvestingActivitiesBuildToSuitLease_b759f078-6e3c-4102-8cde-cbb180fd3b56" xlink:href="xpo-20201231.xsd#xpo_PropertyAndEquipmentAdditionsNonCashInvestingActivitiesBuildToSuitLease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_xpo_PropertyAndEquipmentAdditionsNonCashInvestingActivitiesBuildToSuitLease_b759f078-6e3c-4102-8cde-cbb180fd3b56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LesseeOperatingLeaseLeaseNotYetCommencedUndiscountedAmount_6b6e51b0-0cf4-4639-9963-254ddd50bbc7" xlink:href="xpo-20201231.xsd#xpo_LesseeOperatingLeaseLeaseNotYetCommencedUndiscountedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_xpo_LesseeOperatingLeaseLeaseNotYetCommencedUndiscountedAmount_6b6e51b0-0cf4-4639-9963-254ddd50bbc7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLeaseNotYetCommencedTermOfContract1_6574237d-eda0-4de6-8d1c-17f300fc55f7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLeaseNotYetCommencedTermOfContract1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_us-gaap_LesseeOperatingLeaseLeaseNotYetCommencedTermOfContract1_6574237d-eda0-4de6-8d1c-17f300fc55f7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseAndRentalExpense_6c865bb3-1620-43cc-a1e1-30138cbbef0c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LeaseAndRentalExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_us-gaap_LeaseAndRentalExpense_6c865bb3-1620-43cc-a1e1-30138cbbef0c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_40d05907-b400-4d72-bbb3-a42c5497e698" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_c11b5ac7-fb94-4fab-8a7c-7ae98206e766" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_40d05907-b400-4d72-bbb3-a42c5497e698" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_78cbdbd5-56cf-44a5-b002-5990323a84e2" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_40d05907-b400-4d72-bbb3-a42c5497e698" xlink:to="loc_srt_RangeAxis_78cbdbd5-56cf-44a5-b002-5990323a84e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_78cbdbd5-56cf-44a5-b002-5990323a84e2_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_78cbdbd5-56cf-44a5-b002-5990323a84e2" xlink:to="loc_srt_RangeMember_78cbdbd5-56cf-44a5-b002-5990323a84e2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ed52c85c-4326-4461-999e-3fd58f4419ea" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_78cbdbd5-56cf-44a5-b002-5990323a84e2" xlink:to="loc_srt_RangeMember_ed52c85c-4326-4461-999e-3fd58f4419ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_2ca3b760-eb19-4f2e-b3f5-fe7fc4622029" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ed52c85c-4326-4461-999e-3fd58f4419ea" xlink:to="loc_srt_MinimumMember_2ca3b760-eb19-4f2e-b3f5-fe7fc4622029" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_4e15afec-56f2-4058-804a-f7ce4560759d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ed52c85c-4326-4461-999e-3fd58f4419ea" xlink:to="loc_srt_MaximumMember_4e15afec-56f2-4058-804a-f7ce4560759d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesComponentsofLeaseExpenseandGainRealizedonSalesLeasebackTransactionsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesComponentsofLeaseExpenseandGainRealizedonSalesLeasebackTransactionsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesComponentsofLeaseExpenseandGainRealizedonSalesLeasebackTransactionsDetails" xlink:type="extended" id="i97b14016fe25485bb7427f545a42c0b0_LeasesComponentsofLeaseExpenseandGainRealizedonSalesLeasebackTransactionsDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesSupplementalBalanceSheetInformationRelatedtoLeasesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="extended" id="i420800e01a444fcf8a1280b08b04d05a_LeasesSupplementalBalanceSheetInformationRelatedtoLeasesDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesSupplementalCashFlowInformationRelatedtoLeasesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesSupplementalCashFlowInformationRelatedtoLeasesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesSupplementalCashFlowInformationRelatedtoLeasesDetails" xlink:type="extended" id="i2975c5dc9bd84d0f9efaf37bab230cb7_LeasesSupplementalCashFlowInformationRelatedtoLeasesDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/LeasesMaturitiesofFinancingandOperatingLeaseLiabilitiesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#LeasesMaturitiesofFinancingandOperatingLeaseLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/LeasesMaturitiesofFinancingandOperatingLeaseLiabilitiesDetails" xlink:type="extended" id="if2ab786cd38e44bc87ae54de89e6c7ce_LeasesMaturitiesofFinancingandOperatingLeaseLiabilitiesDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/Goodwill" xlink:type="simple" xlink:href="xpo-20201231.xsd#Goodwill"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/Goodwill" xlink:type="extended" id="iae1067d683b0426691337b1e186fb0b9_Goodwill"/>
  <link:roleRef roleURI="http://www.xpo.com/role/GoodwillTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#GoodwillTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/GoodwillTables" xlink:type="extended" id="i0963749804ab40438f238d5dac871523_GoodwillTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/GoodwillScheduleofGoodwillDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#GoodwillScheduleofGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/GoodwillScheduleofGoodwillDetails" xlink:type="extended" id="i84e4f04e184d448d8b50981a2449678b_GoodwillScheduleofGoodwillDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillLineItems_e136e4ad-c1b4-4077-983e-85bba77991e8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillRollForward_28b569e8-e2f9-480e-ac81-c539f67fdb80" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GoodwillRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillLineItems_e136e4ad-c1b4-4077-983e-85bba77991e8" xlink:to="loc_us-gaap_GoodwillRollForward_28b569e8-e2f9-480e-ac81-c539f67fdb80" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_5427e499-712f-4e6c-b755-59d4913e5f29" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_28b569e8-e2f9-480e-ac81-c539f67fdb80" xlink:to="loc_us-gaap_Goodwill_5427e499-712f-4e6c-b755-59d4913e5f29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_176c2184-9a1e-451b-8094-53cff4ab4f2f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GoodwillForeignCurrencyTranslationGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_28b569e8-e2f9-480e-ac81-c539f67fdb80" xlink:to="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_176c2184-9a1e-451b-8094-53cff4ab4f2f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_c3deff51-4b23-4ec1-8756-ec3cbf4e54d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Goodwill"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillImpairedAccumulatedImpairmentLoss_c269aa01-0526-4ee7-8ec5-0c3972c82562" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GoodwillImpairedAccumulatedImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillLineItems_e136e4ad-c1b4-4077-983e-85bba77991e8" xlink:to="loc_us-gaap_GoodwillImpairedAccumulatedImpairmentLoss_c269aa01-0526-4ee7-8ec5-0c3972c82562" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfGoodwillTable_dd198f6e-d9ed-4a69-b97c-2c2d9de73a08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_GoodwillLineItems_e136e4ad-c1b4-4077-983e-85bba77991e8" xlink:to="loc_us-gaap_ScheduleOfGoodwillTable_dd198f6e-d9ed-4a69-b97c-2c2d9de73a08" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_88dae576-9699-43c7-b97c-26c7a913e892" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfGoodwillTable_dd198f6e-d9ed-4a69-b97c-2c2d9de73a08" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_88dae576-9699-43c7-b97c-26c7a913e892" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_88dae576-9699-43c7-b97c-26c7a913e892_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_88dae576-9699-43c7-b97c-26c7a913e892" xlink:to="loc_us-gaap_SegmentDomain_88dae576-9699-43c7-b97c-26c7a913e892_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_a32e3524-a777-4fbf-8820-e01d957ebfa6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_88dae576-9699-43c7-b97c-26c7a913e892" xlink:to="loc_us-gaap_SegmentDomain_a32e3524-a777-4fbf-8820-e01d957ebfa6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TransportationMember_1781b749-9c7f-4727-a56b-e9f199df01ab" xlink:href="xpo-20201231.xsd#xpo_TransportationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_a32e3524-a777-4fbf-8820-e01d957ebfa6" xlink:to="loc_xpo_TransportationMember_1781b749-9c7f-4727-a56b-e9f199df01ab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ContractLogisticsMember_3a0de425-5027-44ef-b0fb-92a6fa4d38a1" xlink:href="xpo-20201231.xsd#xpo_ContractLogisticsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_a32e3524-a777-4fbf-8820-e01d957ebfa6" xlink:to="loc_xpo_ContractLogisticsMember_3a0de425-5027-44ef-b0fb-92a6fa4d38a1" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/IntangibleAssets" xlink:type="simple" xlink:href="xpo-20201231.xsd#IntangibleAssets"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IntangibleAssets" xlink:type="extended" id="i4b304b6104e348a28e3d241de9657060_IntangibleAssets"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IntangibleAssetsTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#IntangibleAssetsTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IntangibleAssetsTables" xlink:type="extended" id="ie402bc1a0b6c4500aef90544130c904b_IntangibleAssetsTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IntangibleAssetsScheduleofIdentifiableIntangibleAssetsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IntangibleAssetsScheduleofIdentifiableIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IntangibleAssetsScheduleofIdentifiableIntangibleAssetsDetails" xlink:type="extended" id="i68a3209f960e4caca649cbdb86dcac74_IntangibleAssetsScheduleofIdentifiableIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_a2f265a3-f5f9-40c9-ad4f-8950850187be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DefiniteLivedIntangiblesAbstract_628118f2-88fb-4605-a5b2-428e0dcc0fe4" xlink:href="xpo-20201231.xsd#xpo_DefiniteLivedIntangiblesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_a2f265a3-f5f9-40c9-ad4f-8950850187be" xlink:to="loc_xpo_DefiniteLivedIntangiblesAbstract_628118f2-88fb-4605-a5b2-428e0dcc0fe4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_dc0f5763-9d22-4a89-acef-0ba22158e6e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_DefiniteLivedIntangiblesAbstract_628118f2-88fb-4605-a5b2-428e0dcc0fe4" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_dc0f5763-9d22-4a89-acef-0ba22158e6e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_ac9ba2c3-695c-44b2-a675-d34b12c688b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_DefiniteLivedIntangiblesAbstract_628118f2-88fb-4605-a5b2-428e0dcc0fe4" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_ac9ba2c3-695c-44b2-a675-d34b12c688b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_f477452d-5327-4df0-8eef-cdbe5f434e9b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_a2f265a3-f5f9-40c9-ad4f-8950850187be" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_f477452d-5327-4df0-8eef-cdbe5f434e9b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_f11e7328-520c-47e7-933b-6bd7d35a4ac8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_f477452d-5327-4df0-8eef-cdbe5f434e9b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_f11e7328-520c-47e7-933b-6bd7d35a4ac8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_f11e7328-520c-47e7-933b-6bd7d35a4ac8_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_f11e7328-520c-47e7-933b-6bd7d35a4ac8" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_f11e7328-520c-47e7-933b-6bd7d35a4ac8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_827ef2f6-387c-47c5-8752-d58418af560e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_f11e7328-520c-47e7-933b-6bd7d35a4ac8" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_827ef2f6-387c-47c5-8752-d58418af560e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_d411d089-5dd2-4e6e-ac25-5e1604a86f0c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_827ef2f6-387c-47c5-8752-d58418af560e" xlink:to="loc_us-gaap_CustomerRelationshipsMember_d411d089-5dd2-4e6e-ac25-5e1604a86f0c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/IntangibleAssetsEstimatedFutureAmortizationExpenseforAmortizableIntangibleAssetsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IntangibleAssetsEstimatedFutureAmortizationExpenseforAmortizableIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IntangibleAssetsEstimatedFutureAmortizationExpenseforAmortizableIntangibleAssetsDetails" xlink:type="extended" id="ibd096b4b530f4e2fb9920f8c301ed565_IntangibleAssetsEstimatedFutureAmortizationExpenseforAmortizableIntangibleAssetsDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IntangibleAssetsNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IntangibleAssetsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IntangibleAssetsNarrativeDetails" xlink:type="extended" id="i0d4393c31b654fb880029f0667906364_IntangibleAssetsNarrativeDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DerivativeInstruments" xlink:type="simple" xlink:href="xpo-20201231.xsd#DerivativeInstruments"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DerivativeInstruments" xlink:type="extended" id="ia153f95df29946ea83046290db47bd31_DerivativeInstruments"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DerivativeInstrumentsTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#DerivativeInstrumentsTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DerivativeInstrumentsTables" xlink:type="extended" id="idd0cd4896bae46ae96ddd3583cf60dc1_DerivativeInstrumentsTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DerivativeInstrumentsScheduleofDerivativeInstrumentsMeasuredatFairValueinConsolidatedBalanceSheetDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DerivativeInstrumentsScheduleofDerivativeInstrumentsMeasuredatFairValueinConsolidatedBalanceSheetDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DerivativeInstrumentsScheduleofDerivativeInstrumentsMeasuredatFairValueinConsolidatedBalanceSheetDetails" xlink:type="extended" id="ia168de3f817443ecba4b74064f64bd0b_DerivativeInstrumentsScheduleofDerivativeInstrumentsMeasuredatFairValueinConsolidatedBalanceSheetDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativesFairValueLineItems_4c6a4ebc-e743-4b93-9e84-fd71e6e69b80" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativesFairValueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNotionalAmount_47c6f336-69b6-4698-95ae-3093af197c6d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_4c6a4ebc-e743-4b93-9e84-fd71e6e69b80" xlink:to="loc_us-gaap_DerivativeNotionalAmount_47c6f336-69b6-4698-95ae-3093af197c6d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetFairValueNetAbstract_f14c0039-089d-4981-9b97-dd19c2d959fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeAssetFairValueNetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_4c6a4ebc-e743-4b93-9e84-fd71e6e69b80" xlink:to="loc_us-gaap_DerivativeAssetFairValueNetAbstract_f14c0039-089d-4981-9b97-dd19c2d959fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_e0c7e06a-c179-4477-b603-7488695ee01d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeAssetFairValueNetAbstract_f14c0039-089d-4981-9b97-dd19c2d959fd" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_e0c7e06a-c179-4477-b603-7488695ee01d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilityFairValueNetAbstract_4313d102-66fe-4bc8-97eb-2f4723c62167" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeLiabilityFairValueNetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_4c6a4ebc-e743-4b93-9e84-fd71e6e69b80" xlink:to="loc_us-gaap_DerivativeLiabilityFairValueNetAbstract_4313d102-66fe-4bc8-97eb-2f4723c62167" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_9c53ad80-beba-4b63-ac50-b3f58ef3c823" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLiabilityFairValueNetAbstract_4313d102-66fe-4bc8-97eb-2f4723c62167" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_9c53ad80-beba-4b63-ac50-b3f58ef3c823" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_4c6a4ebc-e743-4b93-9e84-fd71e6e69b80" xlink:to="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f1a274b2-f6b3-48ca-b39c-07669937b9d0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f1a274b2-f6b3-48ca-b39c-07669937b9d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_f1a274b2-f6b3-48ca-b39c-07669937b9d0_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f1a274b2-f6b3-48ca-b39c-07669937b9d0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_f1a274b2-f6b3-48ca-b39c-07669937b9d0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_eff0fd81-f773-4d67-8440-78e89c15d5e4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_f1a274b2-f6b3-48ca-b39c-07669937b9d0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_eff0fd81-f773-4d67-8440-78e89c15d5e4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_781f3aca-14de-4adf-80b0-3a6ef6a993c5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_eff0fd81-f773-4d67-8440-78e89c15d5e4" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_781f3aca-14de-4adf-80b0-3a6ef6a993c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_b5033424-99a3-435e-b0b1-a39c5770a26b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_b5033424-99a3-435e-b0b1-a39c5770a26b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_b5033424-99a3-435e-b0b1-a39c5770a26b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_b5033424-99a3-435e-b0b1-a39c5770a26b" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_b5033424-99a3-435e-b0b1-a39c5770a26b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_6bb29baa-347e-4ab4-98fd-efd4977f80ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_b5033424-99a3-435e-b0b1-a39c5770a26b" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_6bb29baa-347e-4ab4-98fd-efd4977f80ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CurrencySwapMember_03f56ef1-73db-4629-8425-a584b0915dd1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CurrencySwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_6bb29baa-347e-4ab4-98fd-efd4977f80ac" xlink:to="loc_us-gaap_CurrencySwapMember_03f56ef1-73db-4629-8425-a584b0915dd1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_0bce6b03-db11-4485-bbdb-24238792cbe4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_6bb29baa-347e-4ab4-98fd-efd4977f80ac" xlink:to="loc_us-gaap_InterestRateSwapMember_0bce6b03-db11-4485-bbdb-24238792cbe4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignExchangeContractMember_f5f115d8-2720-4377-a223-b3f1402635a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignExchangeContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_6bb29baa-347e-4ab4-98fd-efd4977f80ac" xlink:to="loc_us-gaap_ForeignExchangeContractMember_f5f115d8-2720-4377-a223-b3f1402635a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_3143175c-7aa2-4ec2-9cd4-96371132216f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:to="loc_us-gaap_HedgingDesignationAxis_3143175c-7aa2-4ec2-9cd4-96371132216f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_3143175c-7aa2-4ec2-9cd4-96371132216f_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_3143175c-7aa2-4ec2-9cd4-96371132216f" xlink:to="loc_us-gaap_HedgingDesignationDomain_3143175c-7aa2-4ec2-9cd4-96371132216f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_f89c287c-8b85-4bef-9d5c-80739aba6819" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_3143175c-7aa2-4ec2-9cd4-96371132216f" xlink:to="loc_us-gaap_HedgingDesignationDomain_f89c287c-8b85-4bef-9d5c-80739aba6819" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_e7bd679b-7655-421e-83f9-79eb735949ee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_f89c287c-8b85-4bef-9d5c-80739aba6819" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_e7bd679b-7655-421e-83f9-79eb735949ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NondesignatedMember_f1ecc2c3-fd9b-4324-9b9f-c3e6df1bb07f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NondesignatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_f89c287c-8b85-4bef-9d5c-80739aba6819" xlink:to="loc_us-gaap_NondesignatedMember_f1ecc2c3-fd9b-4324-9b9f-c3e6df1bb07f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_064552ed-e80a-4642-ba42-710ea31f96a4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_096b1da8-bb5e-4422-a2e3-d6c85ba87b61" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_064552ed-e80a-4642-ba42-710ea31f96a4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_064552ed-e80a-4642-ba42-710ea31f96a4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_064552ed-e80a-4642-ba42-710ea31f96a4" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_064552ed-e80a-4642-ba42-710ea31f96a4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_064552ed-e80a-4642-ba42-710ea31f96a4" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_OtherLongTermAssetsandOtherLongTermLiabilitiesMember_e1141876-8ee2-4fa6-93d9-7262f2bc7ec6" xlink:href="xpo-20201231.xsd#xpo_OtherLongTermAssetsandOtherLongTermLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_xpo_OtherLongTermAssetsandOtherLongTermLiabilitiesMember_e1141876-8ee2-4fa6-93d9-7262f2bc7ec6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncurrentAssetsMember_d94dc09d-deaf-4dc8-8064-ffa4554d87bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNoncurrentAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_us-gaap_OtherNoncurrentAssetsMember_d94dc09d-deaf-4dc8-8064-ffa4554d87bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncurrentLiabilitiesMember_15f3e83a-6716-4c06-89f0-66c13cb072f4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNoncurrentLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_us-gaap_OtherNoncurrentLiabilitiesMember_15f3e83a-6716-4c06-89f0-66c13cb072f4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_OtherCurrentAssetsandOtherCurrentLiabilitiesMember_6747fb17-cde8-44bd-9c91-05cc023324eb" xlink:href="xpo-20201231.xsd#xpo_OtherCurrentAssetsandOtherCurrentLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_xpo_OtherCurrentAssetsandOtherCurrentLiabilitiesMember_6747fb17-cde8-44bd-9c91-05cc023324eb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCurrentAssetsMember_44365116-08ad-4a75-a8ed-22e7d02bc612" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherCurrentAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_us-gaap_OtherCurrentAssetsMember_44365116-08ad-4a75-a8ed-22e7d02bc612" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCurrentLiabilitiesMember_c8be547d-d7cc-47c9-a2b1-03ee80818073" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherCurrentLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_deefceed-79ac-4097-b45b-283fabfff950" xlink:to="loc_us-gaap_OtherCurrentLiabilitiesMember_c8be547d-d7cc-47c9-a2b1-03ee80818073" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DerivativeInstrumentsScheduleofGainsandLossesRecognizedonConsolidatedStatementsofOperationsforDerivativeInstrumentsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DerivativeInstrumentsScheduleofGainsandLossesRecognizedonConsolidatedStatementsofOperationsforDerivativeInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DerivativeInstrumentsScheduleofGainsandLossesRecognizedonConsolidatedStatementsofOperationsforDerivativeInstrumentsDetails" xlink:type="extended" id="icc33c93aaddd4dfdb99f8f1f80d40c1e_DerivativeInstrumentsScheduleofGainsandLossesRecognizedonConsolidatedStatementsofOperationsforDerivativeInstrumentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax_057bfce1-f8bf-4986-a300-4934f3ec449a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax_057bfce1-f8bf-4986-a300-4934f3ec449a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax_efc498a0-fa35-432e-a97f-2adf24259472" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax_efc498a0-fa35-432e-a97f-2adf24259472" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeExcludedComponentGainLossRecognizedInEarnings_0ed944bd-1fc9-4bfd-b333-c5dcb2d1a8bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeExcludedComponentGainLossRecognizedInEarnings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:to="loc_us-gaap_DerivativeExcludedComponentGainLossRecognizedInEarnings_0ed944bd-1fc9-4bfd-b333-c5dcb2d1a8bb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossDerivativeExcludedComponentIncreaseDecreaseBeforeAdjustmentsAndTax_397c950f-4911-43a5-88dd-78c9164c7f84" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossDerivativeExcludedComponentIncreaseDecreaseBeforeAdjustmentsAndTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossDerivativeExcludedComponentIncreaseDecreaseBeforeAdjustmentsAndTax_397c950f-4911-43a5-88dd-78c9164c7f84" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_4810bb9f-8327-4efa-8ee0-c6cc384daf59" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_0918897d-70da-4807-be15-855814295de6" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_4810bb9f-8327-4efa-8ee0-c6cc384daf59" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_4810bb9f-8327-4efa-8ee0-c6cc384daf59" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_5497f2a5-6c8a-4a06-b036-855217a5ec1e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_e5e6a3f0-d062-4f7f-ad6b-6f4bbbd17a28" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_5497f2a5-6c8a-4a06-b036-855217a5ec1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CurrencySwapMember_8acb8eae-f619-4afb-bbbb-8360c6d32c34" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CurrencySwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_5497f2a5-6c8a-4a06-b036-855217a5ec1e" xlink:to="loc_us-gaap_CurrencySwapMember_8acb8eae-f619-4afb-bbbb-8360c6d32c34" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_822185fa-3d13-4411-8132-fef6e82b48e9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_5497f2a5-6c8a-4a06-b036-855217a5ec1e" xlink:to="loc_us-gaap_InterestRateSwapMember_822185fa-3d13-4411-8132-fef6e82b48e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_511caf20-7c60-4ea0-a1ad-2d6747c403c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_4810bb9f-8327-4efa-8ee0-c6cc384daf59" xlink:to="loc_us-gaap_HedgingDesignationAxis_511caf20-7c60-4ea0-a1ad-2d6747c403c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_511caf20-7c60-4ea0-a1ad-2d6747c403c4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_511caf20-7c60-4ea0-a1ad-2d6747c403c4" xlink:to="loc_us-gaap_HedgingDesignationDomain_511caf20-7c60-4ea0-a1ad-2d6747c403c4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_10b746e6-8026-4655-8344-66681798e2bd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_511caf20-7c60-4ea0-a1ad-2d6747c403c4" xlink:to="loc_us-gaap_HedgingDesignationDomain_10b746e6-8026-4655-8344-66681798e2bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_08f970b0-2b34-480d-bdac-ae57d7fa36de" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_10b746e6-8026-4655-8344-66681798e2bd" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_08f970b0-2b34-480d-bdac-ae57d7fa36de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NondesignatedMember_1d77f2fc-8ca6-4a81-a86f-3d424837661c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NondesignatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_10b746e6-8026-4655-8344-66681798e2bd" xlink:to="loc_us-gaap_NondesignatedMember_1d77f2fc-8ca6-4a81-a86f-3d424837661c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DerivativeInstrumentsNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DerivativeInstrumentsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DerivativeInstrumentsNarrativeDetails" xlink:type="extended" id="i3f337dc0c1b4465cb154fceda7136cf4_DerivativeInstrumentsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_dc95e328-1af0-46c0-9c94-560f1337ab7d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTermOfContract_1344ed59-309c-41d5-9c74-f63484461b03" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_dc95e328-1af0-46c0-9c94-560f1337ab7d" xlink:to="loc_us-gaap_DerivativeTermOfContract_1344ed59-309c-41d5-9c74-f63484461b03" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNumberOfInstrumentsHeld_d2217c4b-8227-4077-8e97-bf82ad4602b4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeNumberOfInstrumentsHeld"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_dc95e328-1af0-46c0-9c94-560f1337ab7d" xlink:to="loc_us-gaap_DerivativeNumberOfInstrumentsHeld_d2217c4b-8227-4077-8e97-bf82ad4602b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_f826c686-3a33-4391-b43c-f1e31696674c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_dc95e328-1af0-46c0-9c94-560f1337ab7d" xlink:to="loc_us-gaap_DerivativeTable_f826c686-3a33-4391-b43c-f1e31696674c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_1c3c0831-989d-420d-a04d-5ac47ec781e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_f826c686-3a33-4391-b43c-f1e31696674c" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_1c3c0831-989d-420d-a04d-5ac47ec781e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_1c3c0831-989d-420d-a04d-5ac47ec781e2_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_1c3c0831-989d-420d-a04d-5ac47ec781e2" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_1c3c0831-989d-420d-a04d-5ac47ec781e2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_71184df4-4529-493b-a9c2-955e4b15cff2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_1c3c0831-989d-420d-a04d-5ac47ec781e2" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_71184df4-4529-493b-a9c2-955e4b15cff2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignExchangeForwardMember_55611490-f3c8-4781-b514-5f26c6f2acd5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignExchangeForwardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_71184df4-4529-493b-a9c2-955e4b15cff2" xlink:to="loc_us-gaap_ForeignExchangeForwardMember_55611490-f3c8-4781-b514-5f26c6f2acd5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_7c3351f8-6223-4fc2-882d-77b832a2bb98" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_f826c686-3a33-4391-b43c-f1e31696674c" xlink:to="loc_us-gaap_HedgingDesignationAxis_7c3351f8-6223-4fc2-882d-77b832a2bb98" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_7c3351f8-6223-4fc2-882d-77b832a2bb98_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_7c3351f8-6223-4fc2-882d-77b832a2bb98" xlink:to="loc_us-gaap_HedgingDesignationDomain_7c3351f8-6223-4fc2-882d-77b832a2bb98_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_2b712503-bb5d-43f7-acef-cfa981a35941" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_7c3351f8-6223-4fc2-882d-77b832a2bb98" xlink:to="loc_us-gaap_HedgingDesignationDomain_2b712503-bb5d-43f7-acef-cfa981a35941" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NondesignatedMember_2ab5c9fc-90d0-4a7f-835f-c935632f494d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NondesignatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_2b712503-bb5d-43f7-acef-cfa981a35941" xlink:to="loc_us-gaap_NondesignatedMember_2ab5c9fc-90d0-4a7f-835f-c935632f494d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_711e4c79-b754-4bda-ba66-74a5aa9fda5a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_f826c686-3a33-4391-b43c-f1e31696674c" xlink:to="loc_srt_RangeAxis_711e4c79-b754-4bda-ba66-74a5aa9fda5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_711e4c79-b754-4bda-ba66-74a5aa9fda5a_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_711e4c79-b754-4bda-ba66-74a5aa9fda5a" xlink:to="loc_srt_RangeMember_711e4c79-b754-4bda-ba66-74a5aa9fda5a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f4e07af9-b4be-4b5c-ae99-faa876b5f0fd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_711e4c79-b754-4bda-ba66-74a5aa9fda5a" xlink:to="loc_srt_RangeMember_f4e07af9-b4be-4b5c-ae99-faa876b5f0fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_8a47b7f3-17db-4a77-8960-6137101778e6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f4e07af9-b4be-4b5c-ae99-faa876b5f0fd" xlink:to="loc_srt_MaximumMember_8a47b7f3-17db-4a77-8960-6137101778e6" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/Debt" xlink:type="simple" xlink:href="xpo-20201231.xsd#Debt"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/Debt" xlink:type="extended" id="idfd24bd829024d0da89f534c3ed7804d_Debt"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtTables" xlink:type="extended" id="i7c4844d7836a49b5a2e05a56d650ffc3_DebtTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtScheduleofDebtDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtScheduleofDebtDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtScheduleofDebtDetails" xlink:type="extended" id="i6db5fa3d9e314c1aaa72a53c6b92d2fe_DebtScheduleofDebtDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_983537a4-4343-43cc-bc85-b5b840412635" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_LongTermDebt_983537a4-4343-43cc-bc85-b5b840412635" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_FinanceLeasesAssetFinancingAndOther_62a82147-88dc-4ab1-9cb4-fcd78faed798" xlink:href="xpo-20201231.xsd#xpo_FinanceLeasesAssetFinancingAndOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_xpo_FinanceLeasesAssetFinancingAndOther_62a82147-88dc-4ab1-9cb4-fcd78faed798" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ShortTermDebtAndCurrentMaturitiesOfLongTermDebt_752b3cc8-919a-4d2b-b042-6591a9baa6c0" xlink:href="xpo-20201231.xsd#xpo_ShortTermDebtAndCurrentMaturitiesOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_xpo_ShortTermDebtAndCurrentMaturitiesOfLongTermDebt_752b3cc8-919a-4d2b-b042-6591a9baa6c0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtAndCapitalLeaseObligations_ad97ee2d-68b8-4459-9218-b6ffeff0c881" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtAndCapitalLeaseObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_DebtAndCapitalLeaseObligations_ad97ee2d-68b8-4459-9218-b6ffeff0c881" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LongTermDebtAndCapitalLeaseObligationsExcludingCurrentMaturitiesAndShortTermDebt_3a0b086b-252d-4f7c-bcb7-5867dd1f7130" xlink:href="xpo-20201231.xsd#xpo_LongTermDebtAndCapitalLeaseObligationsExcludingCurrentMaturitiesAndShortTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_xpo_LongTermDebtAndCapitalLeaseObligationsExcludingCurrentMaturitiesAndShortTermDebt_3a0b086b-252d-4f7c-bcb7-5867dd1f7130" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_b0911c0e-5be3-45f9-8a1d-0233b5048c6a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_LongTermDebtFairValue_b0911c0e-5be3-45f9-8a1d-0233b5048c6a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_3e4ba143-d31f-4506-ad4c-6dedf0ae07c5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_LongTermDebtCurrent_3e4ba143-d31f-4506-ad4c-6dedf0ae07c5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_8ee004ac-439c-461a-822c-c02024c53ae9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_8ee004ac-439c-461a-822c-c02024c53ae9" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_5c2a950b-1cca-493d-adda-d4727cd60f3a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_5c2a950b-1cca-493d-adda-d4727cd60f3a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_6467c3be-6558-4a17-b9de-52d13e679c94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_1ec5da90-6f85-4f38-8a5e-60f5b3fff4de" xlink:to="loc_us-gaap_DebtInstrumentTable_6467c3be-6558-4a17-b9de-52d13e679c94" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_dded4ae8-6da6-46c1-808b-f05256dfa624" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6467c3be-6558-4a17-b9de-52d13e679c94" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_dded4ae8-6da6-46c1-808b-f05256dfa624" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_dded4ae8-6da6-46c1-808b-f05256dfa624_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_dded4ae8-6da6-46c1-808b-f05256dfa624" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_dded4ae8-6da6-46c1-808b-f05256dfa624_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_a2bd8ddf-b34d-4bd8-bab2-026095cf9837" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_dded4ae8-6da6-46c1-808b-f05256dfa624" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_a2bd8ddf-b34d-4bd8-bab2-026095cf9837" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_2f6e9d83-0c30-4a18-8c7d-aa5f170dfb37" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_a2bd8ddf-b34d-4bd8-bab2-026095cf9837" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_2f6e9d83-0c30-4a18-8c7d-aa5f170dfb37" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_9d6821fa-c6f6-4376-8592-d22be4ae8625" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_2f6e9d83-0c30-4a18-8c7d-aa5f170dfb37" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_9d6821fa-c6f6-4376-8592-d22be4ae8625" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_c0bd6831-a1a3-4e59-ae8d-8522fe311835" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6467c3be-6558-4a17-b9de-52d13e679c94" xlink:to="loc_us-gaap_CreditFacilityAxis_c0bd6831-a1a3-4e59-ae8d-8522fe311835" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_c0bd6831-a1a3-4e59-ae8d-8522fe311835_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_c0bd6831-a1a3-4e59-ae8d-8522fe311835" xlink:to="loc_us-gaap_CreditFacilityDomain_c0bd6831-a1a3-4e59-ae8d-8522fe311835_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_583d81bb-8f5c-46b3-8b64-c13ad79d0ac8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_c0bd6831-a1a3-4e59-ae8d-8522fe311835" xlink:to="loc_us-gaap_CreditFacilityDomain_583d81bb-8f5c-46b3-8b64-c13ad79d0ac8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ABLFacilityMember_2dd9316e-0a94-4e96-8b0e-85bd9485c2cf" xlink:href="xpo-20201231.xsd#xpo_ABLFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_583d81bb-8f5c-46b3-8b64-c13ad79d0ac8" xlink:to="loc_xpo_ABLFacilityMember_2dd9316e-0a94-4e96-8b0e-85bd9485c2cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_f0546c56-200e-441c-9a0d-ba44c6cadb90" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_6467c3be-6558-4a17-b9de-52d13e679c94" xlink:to="loc_us-gaap_DebtInstrumentAxis_f0546c56-200e-441c-9a0d-ba44c6cadb90" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f0546c56-200e-441c-9a0d-ba44c6cadb90_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_f0546c56-200e-441c-9a0d-ba44c6cadb90" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f0546c56-200e-441c-9a0d-ba44c6cadb90_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_f0546c56-200e-441c-9a0d-ba44c6cadb90" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TermLoanFacilityMember_39e904b6-d68c-4ba8-adfc-1296fe448271" xlink:href="xpo-20201231.xsd#xpo_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_TermLoanFacilityMember_39e904b6-d68c-4ba8-adfc-1296fe448271" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2022Member_4e10657d-5b08-45e3-b0c6-06e498f1d84c" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2022Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_SeniorNotesDue2022Member_4e10657d-5b08-45e3-b0c6-06e498f1d84c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2023Member_e59c44c0-bf86-4f50-8b87-2756b3c50f35" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2023Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_SeniorNotesDue2023Member_e59c44c0-bf86-4f50-8b87-2756b3c50f35" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2024Member_66955ca6-8dab-45b6-9c48-7ae52b2f4b8c" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2024Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_SeniorNotesDue2024Member_66955ca6-8dab-45b6-9c48-7ae52b2f4b8c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2025Member_beccfecd-45f5-4c09-b833-afad73e2a731" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2025Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_SeniorNotesDue2025Member_beccfecd-45f5-4c09-b833-afad73e2a731" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorDebenturesDue2034Member_6ab6261e-49f6-4a16-b112-735d1a6d0141" xlink:href="xpo-20201231.xsd#xpo_SeniorDebenturesDue2034Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_SeniorDebenturesDue2034Member_6ab6261e-49f6-4a16-b112-735d1a6d0141" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EuropeanTradeSecuritizationProgramMember_c3ddf1a7-9174-4e77-aa0a-13c9c4332a92" xlink:href="xpo-20201231.xsd#xpo_EuropeanTradeSecuritizationProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_EuropeanTradeSecuritizationProgramMember_c3ddf1a7-9174-4e77-aa0a-13c9c4332a92" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_AssetFinanceDebtMember_c348af33-635d-4b0a-92f6-8ccc50be78df" xlink:href="xpo-20201231.xsd#xpo_AssetFinanceDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_a49299ea-c716-4654-844d-51c2fee95f92" xlink:to="loc_xpo_AssetFinanceDebtMember_c348af33-635d-4b0a-92f6-8ccc50be78df" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtFairValueofDebtDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtFairValueofDebtDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtFairValueofDebtDetails" xlink:type="extended" id="i481f4f9024b14c29b321b3bf85d04a34_DebtFairValueofDebtDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_a378b863-1836-4315-ada7-973d61260361" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFairValue_16234a76-3bbd-4e66-a8a7-b47edbfd0e44" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_a378b863-1836-4315-ada7-973d61260361" xlink:to="loc_us-gaap_DebtInstrumentFairValue_16234a76-3bbd-4e66-a8a7-b47edbfd0e44" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_078da733-b31a-4ac6-8cd2-c493bcc95892" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_a378b863-1836-4315-ada7-973d61260361" xlink:to="loc_us-gaap_DebtInstrumentTable_078da733-b31a-4ac6-8cd2-c493bcc95892" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_6ecfe958-11f9-48d3-9603-337904f3a56e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_078da733-b31a-4ac6-8cd2-c493bcc95892" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_6ecfe958-11f9-48d3-9603-337904f3a56e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6ecfe958-11f9-48d3-9603-337904f3a56e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_6ecfe958-11f9-48d3-9603-337904f3a56e" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6ecfe958-11f9-48d3-9603-337904f3a56e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b8fe3b5-9eb0-4d96-821e-bdb2b59fb218" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_6ecfe958-11f9-48d3-9603-337904f3a56e" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b8fe3b5-9eb0-4d96-821e-bdb2b59fb218" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_1a7a0ca9-1423-4b40-ba1d-9527c7a64982" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b8fe3b5-9eb0-4d96-821e-bdb2b59fb218" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_1a7a0ca9-1423-4b40-ba1d-9527c7a64982" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_7183a59d-b8d5-4eca-a04d-b1a09d21646d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4b8fe3b5-9eb0-4d96-821e-bdb2b59fb218" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_7183a59d-b8d5-4eca-a04d-b1a09d21646d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtScheduleofDebtMaturityDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtScheduleofDebtMaturityDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtScheduleofDebtMaturityDetails" xlink:type="extended" id="i119be56436874fcebe76e913345ed7ce_DebtScheduleofDebtMaturityDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtABLFacilityDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtABLFacilityDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtABLFacilityDetails" xlink:type="extended" id="ie4e56131b87f4d36be6b6ddbb9149459_DebtABLFacilityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_04139eeb-e3f0-444c-a6d6-8219512ac772" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_04139eeb-e3f0-444c-a6d6-8219512ac772" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCapacityAvailableForSpecificPurposeOtherThanForTradePurchases_8017fc11-482d-45d2-8b17-8ffb2f52a98b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityCapacityAvailableForSpecificPurposeOtherThanForTradePurchases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LineOfCreditFacilityCapacityAvailableForSpecificPurposeOtherThanForTradePurchases_8017fc11-482d-45d2-8b17-8ffb2f52a98b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LineOfCreditFacilityBorrowingBaseMaximumPortionAttributableToEquipmentAndRollingStockPercentage_2ca905ad-6b7e-449a-b163-1b184c69207b" xlink:href="xpo-20201231.xsd#xpo_LineOfCreditFacilityBorrowingBaseMaximumPortionAttributableToEquipmentAndRollingStockPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_xpo_LineOfCreditFacilityBorrowingBaseMaximumPortionAttributableToEquipmentAndRollingStockPercentage_2ca905ad-6b7e-449a-b163-1b184c69207b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_af35f835-abd4-4b76-ab07-cd90f82cd4ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_af35f835-abd4-4b76-ab07-cd90f82cd4ed" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_c6abb1ff-e183-47a1-8a4b-6bd988f843f9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_c6abb1ff-e183-47a1-8a4b-6bd988f843f9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_1216c220-f414-4f27-9909-2c5c0c77fb2d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LineOfCredit_1216c220-f414-4f27-9909-2c5c0c77fb2d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_61161130-abc3-46dc-a1bc-3956a2c2511f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_61161130-abc3-46dc-a1bc-3956a2c2511f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_5aec1173-a063-41dc-b75b-1042e7bfb151" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_5aec1173-a063-41dc-b75b-1042e7bfb151" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_FixedChargeCoverageRatio_3d6aad9b-078b-403b-9f2d-c423a541832a" xlink:href="xpo-20201231.xsd#xpo_FixedChargeCoverageRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_xpo_FixedChargeCoverageRatio_3d6aad9b-078b-403b-9f2d-c423a541832a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_015dddd3-0707-495b-b88e-14b01d9d3849" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0c317fc5-fc73-4963-bbc2-464bd89603d5" xlink:to="loc_us-gaap_DebtInstrumentTable_015dddd3-0707-495b-b88e-14b01d9d3849" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_0548b5e2-8fce-4e05-842b-1ffe8497143d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_015dddd3-0707-495b-b88e-14b01d9d3849" xlink:to="loc_us-gaap_CreditFacilityAxis_0548b5e2-8fce-4e05-842b-1ffe8497143d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_0548b5e2-8fce-4e05-842b-1ffe8497143d_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_0548b5e2-8fce-4e05-842b-1ffe8497143d" xlink:to="loc_us-gaap_CreditFacilityDomain_0548b5e2-8fce-4e05-842b-1ffe8497143d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_fb1fbf98-8e74-4987-9a31-0f5fa6fafeb7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_0548b5e2-8fce-4e05-842b-1ffe8497143d" xlink:to="loc_us-gaap_CreditFacilityDomain_fb1fbf98-8e74-4987-9a31-0f5fa6fafeb7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ABLFacilityMember_e162573d-35d7-43da-ad1d-8537ff7d6c20" xlink:href="xpo-20201231.xsd#xpo_ABLFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_fb1fbf98-8e74-4987-9a31-0f5fa6fafeb7" xlink:to="loc_xpo_ABLFacilityMember_e162573d-35d7-43da-ad1d-8537ff7d6c20" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_e73f416f-8981-42bd-8ad5-f6b80ac952b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_015dddd3-0707-495b-b88e-14b01d9d3849" xlink:to="loc_us-gaap_VariableRateAxis_e73f416f-8981-42bd-8ad5-f6b80ac952b3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_e73f416f-8981-42bd-8ad5-f6b80ac952b3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_e73f416f-8981-42bd-8ad5-f6b80ac952b3" xlink:to="loc_us-gaap_VariableRateDomain_e73f416f-8981-42bd-8ad5-f6b80ac952b3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_30868213-a65e-4f82-81e9-cb2ab2d2f0e3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_e73f416f-8981-42bd-8ad5-f6b80ac952b3" xlink:to="loc_us-gaap_VariableRateDomain_30868213-a65e-4f82-81e9-cb2ab2d2f0e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_191d75f7-4334-4f4e-8deb-a201762ad910" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_30868213-a65e-4f82-81e9-cb2ab2d2f0e3" xlink:to="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_191d75f7-4334-4f4e-8deb-a201762ad910" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_e46786d4-1339-44fb-9e9a-a58f45a3ec25" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_30868213-a65e-4f82-81e9-cb2ab2d2f0e3" xlink:to="loc_us-gaap_BaseRateMember_e46786d4-1339-44fb-9e9a-a58f45a3ec25" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ac5cafd2-1466-4cac-8265-5cc6fac99690" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_015dddd3-0707-495b-b88e-14b01d9d3849" xlink:to="loc_srt_RangeAxis_ac5cafd2-1466-4cac-8265-5cc6fac99690" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ac5cafd2-1466-4cac-8265-5cc6fac99690_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ac5cafd2-1466-4cac-8265-5cc6fac99690" xlink:to="loc_srt_RangeMember_ac5cafd2-1466-4cac-8265-5cc6fac99690_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_fb6478e7-707d-48f8-81a9-bf902a729cf5" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ac5cafd2-1466-4cac-8265-5cc6fac99690" xlink:to="loc_srt_RangeMember_fb6478e7-707d-48f8-81a9-bf902a729cf5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_6cd491ae-966a-4278-80f3-4c0470d2a418" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fb6478e7-707d-48f8-81a9-bf902a729cf5" xlink:to="loc_srt_MinimumMember_6cd491ae-966a-4278-80f3-4c0470d2a418" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_3e6ecc04-f046-4774-ada5-42ff5ba8c57a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fb6478e7-707d-48f8-81a9-bf902a729cf5" xlink:to="loc_srt_MaximumMember_3e6ecc04-f046-4774-ada5-42ff5ba8c57a" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtSecuredDebtDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtSecuredDebtDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtSecuredDebtDetails" xlink:type="extended" id="i6c3211fb58fb4a119d27014e374cdf53_DebtSecuredDebtDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_0bd37ed0-7dac-4cac-85b2-27b2ae220491" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_cab42db9-a3e3-4f3b-9ab5-2b5e9f4e133e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0bd37ed0-7dac-4cac-85b2-27b2ae220491" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_cab42db9-a3e3-4f3b-9ab5-2b5e9f4e133e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_50f305a4-fd48-453f-bac2-461c2d74d8ae" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0bd37ed0-7dac-4cac-85b2-27b2ae220491" xlink:to="loc_us-gaap_DebtInstrumentTerm_50f305a4-fd48-453f-bac2-461c2d74d8ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DebtInstrumentRenewalTerm_d039c1f8-caa7-4cd8-ab46-143fef1a66d2" xlink:href="xpo-20201231.xsd#xpo_DebtInstrumentRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0bd37ed0-7dac-4cac-85b2-27b2ae220491" xlink:to="loc_xpo_DebtInstrumentRenewalTerm_d039c1f8-caa7-4cd8-ab46-143fef1a66d2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_299af695-d0c0-4c50-a9a6-a78133e8b5ef" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_0bd37ed0-7dac-4cac-85b2-27b2ae220491" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_299af695-d0c0-4c50-a9a6-a78133e8b5ef" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_906048c9-e94c-443b-9592-5e42c9eaeea8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_299af695-d0c0-4c50-a9a6-a78133e8b5ef" xlink:to="loc_us-gaap_CreditFacilityAxis_906048c9-e94c-443b-9592-5e42c9eaeea8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_906048c9-e94c-443b-9592-5e42c9eaeea8_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_906048c9-e94c-443b-9592-5e42c9eaeea8" xlink:to="loc_us-gaap_CreditFacilityDomain_906048c9-e94c-443b-9592-5e42c9eaeea8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_d43b2c89-2a3a-4b04-9582-1d949c53620b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_906048c9-e94c-443b-9592-5e42c9eaeea8" xlink:to="loc_us-gaap_CreditFacilityDomain_d43b2c89-2a3a-4b04-9582-1d949c53620b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SecuredTermLoanFacilityMember_49b9f5b2-2ef5-415d-9bc6-575b3c25fbb8" xlink:href="xpo-20201231.xsd#xpo_SecuredTermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_d43b2c89-2a3a-4b04-9582-1d949c53620b" xlink:to="loc_xpo_SecuredTermLoanFacilityMember_49b9f5b2-2ef5-415d-9bc6-575b3c25fbb8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LetterOfCreditMember_9c6e1cf3-1dc0-4e68-bdfb-9e1ac301a4c9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LetterOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_d43b2c89-2a3a-4b04-9582-1d949c53620b" xlink:to="loc_us-gaap_LetterOfCreditMember_9c6e1cf3-1dc0-4e68-bdfb-9e1ac301a4c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_UncommittedSecuredLetterOfCreditFacilityMember_956aaea8-64d0-40ed-adcf-7cadba07b37e" xlink:href="xpo-20201231.xsd#xpo_UncommittedSecuredLetterOfCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_d43b2c89-2a3a-4b04-9582-1d949c53620b" xlink:to="loc_xpo_UncommittedSecuredLetterOfCreditFacilityMember_956aaea8-64d0-40ed-adcf-7cadba07b37e" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtTermLoanFacilityNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtTermLoanFacilityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtTermLoanFacilityNarrativeDetails" xlink:type="extended" id="i72ce445699d74034b73edf576d569cd7_DebtTermLoanFacilityNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_c5851399-8a34-4bc8-b249-a6862ccb2b08" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_c5851399-8a34-4bc8-b249-a6862ccb2b08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscount_8aafc57c-60cb-440e-a5bd-b57c700d8253" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentUnamortizedDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscount_8aafc57c-60cb-440e-a5bd-b57c700d8253" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DebtIssuancePriceAsPercentageOfParValue_23ece572-84e9-44ac-a29f-689c204701b8" xlink:href="xpo-20201231.xsd#xpo_DebtIssuancePriceAsPercentageOfParValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_xpo_DebtIssuancePriceAsPercentageOfParValue_23ece572-84e9-44ac-a29f-689c204701b8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_2b757625-1c31-44c7-a321-8181550cd51a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_2b757625-1c31-44c7-a321-8181550cd51a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_a5fc309b-661e-448b-ad5f-d27720b78d13" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_LongTermDebt_a5fc309b-661e-448b-ad5f-d27720b78d13" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_da24f1a5-99bc-4bb5-b020-dc5f0cb247f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_da24f1a5-99bc-4bb5-b020-dc5f0cb247f6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_LineofCreditFacilityPrepaymentasPercentageofExcessCashFlow_d141901a-0157-424b-93c2-94f108414733" xlink:href="xpo-20201231.xsd#xpo_LineofCreditFacilityPrepaymentasPercentageofExcessCashFlow"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_xpo_LineofCreditFacilityPrepaymentasPercentageofExcessCashFlow_d141901a-0157-424b-93c2-94f108414733" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DebtInstrumentCovenantComplianceLeverageRatioRequired_6ae64eb0-306c-4848-a689-2446bbd48453" xlink:href="xpo-20201231.xsd#xpo_DebtInstrumentCovenantComplianceLeverageRatioRequired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_xpo_DebtInstrumentCovenantComplianceLeverageRatioRequired_6ae64eb0-306c-4848-a689-2446bbd48453" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_0fcbeb33-794c-4175-9078-ad4ba97538b4" xlink:to="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_5cd93acb-9790-4624-8ba3-1514c69f76cb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:to="loc_us-gaap_CreditFacilityAxis_5cd93acb-9790-4624-8ba3-1514c69f76cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_5cd93acb-9790-4624-8ba3-1514c69f76cb_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_5cd93acb-9790-4624-8ba3-1514c69f76cb" xlink:to="loc_us-gaap_CreditFacilityDomain_5cd93acb-9790-4624-8ba3-1514c69f76cb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_5cd93acb-9790-4624-8ba3-1514c69f76cb" xlink:to="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TermLoanFacilityMember_b0daac19-5c8a-4b2e-bcc1-62bdf0b41a58" xlink:href="xpo-20201231.xsd#xpo_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:to="loc_xpo_TermLoanFacilityMember_b0daac19-5c8a-4b2e-bcc1-62bdf0b41a58" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_IncrementalLoanFacilityMember_9f6e2e9e-4087-4b45-b101-541452e69550" xlink:href="xpo-20201231.xsd#xpo_IncrementalLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:to="loc_xpo_IncrementalLoanFacilityMember_9f6e2e9e-4087-4b45-b101-541452e69550" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TermLoanFacilityScenario1Member_25920a04-5b18-4010-ba30-ef2f8e346bb3" xlink:href="xpo-20201231.xsd#xpo_TermLoanFacilityScenario1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:to="loc_xpo_TermLoanFacilityScenario1Member_25920a04-5b18-4010-ba30-ef2f8e346bb3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TermLoanFacilityScenario2Member_4e2f721e-4585-41d1-8302-cf19b30fd7a3" xlink:href="xpo-20201231.xsd#xpo_TermLoanFacilityScenario2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_28679f54-a623-4aa8-ba6d-010ae04f13d7" xlink:to="loc_xpo_TermLoanFacilityScenario2Member_4e2f721e-4585-41d1-8302-cf19b30fd7a3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_35f47b09-30df-43e9-ad45-824ea76e607b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_35f47b09-30df-43e9-ad45-824ea76e607b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_35f47b09-30df-43e9-ad45-824ea76e607b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_35f47b09-30df-43e9-ad45-824ea76e607b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_35f47b09-30df-43e9-ad45-824ea76e607b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_92125920-abd7-4357-8ba1-357bb653d667" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_35f47b09-30df-43e9-ad45-824ea76e607b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_92125920-abd7-4357-8ba1-357bb653d667" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_c0d8b1d4-d101-40e1-96b3-d51fedee758b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_92125920-abd7-4357-8ba1-357bb653d667" xlink:to="loc_us-gaap_SecuredDebtMember_c0d8b1d4-d101-40e1-96b3-d51fedee758b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:to="loc_us-gaap_DebtInstrumentAxis_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_530f222d-6ff9-484f-acbe-69ac52cb0544" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_6aa9c4dc-e1d8-47ae-b02c-25d2765c57ac" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_530f222d-6ff9-484f-acbe-69ac52cb0544" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_AmendmentNo.2toCreditAgreementMember_c99f2145-fbd4-46e0-be7e-f94c07cdc7fd" xlink:href="xpo-20201231.xsd#xpo_AmendmentNo.2toCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_530f222d-6ff9-484f-acbe-69ac52cb0544" xlink:to="loc_xpo_AmendmentNo.2toCreditAgreementMember_c99f2145-fbd4-46e0-be7e-f94c07cdc7fd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_AmendmentNo.3toCreditAgreementMember_bcade54a-3598-4252-a7d3-772ece19528b" xlink:href="xpo-20201231.xsd#xpo_AmendmentNo.3toCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_530f222d-6ff9-484f-acbe-69ac52cb0544" xlink:to="loc_xpo_AmendmentNo.3toCreditAgreementMember_bcade54a-3598-4252-a7d3-772ece19528b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_800c1435-e3fd-4201-b1d0-997298aced1f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f3c6e1d6-a613-49ee-9926-7dca5ef4cc3b" xlink:to="loc_srt_RangeAxis_800c1435-e3fd-4201-b1d0-997298aced1f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_800c1435-e3fd-4201-b1d0-997298aced1f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_800c1435-e3fd-4201-b1d0-997298aced1f" xlink:to="loc_srt_RangeMember_800c1435-e3fd-4201-b1d0-997298aced1f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_073de263-640d-4f5a-ada7-e32d30fe508d" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_800c1435-e3fd-4201-b1d0-997298aced1f" xlink:to="loc_srt_RangeMember_073de263-640d-4f5a-ada7-e32d30fe508d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_faf1d162-b42a-4e69-946f-423bf77039a9" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_073de263-640d-4f5a-ada7-e32d30fe508d" xlink:to="loc_srt_MinimumMember_faf1d162-b42a-4e69-946f-423bf77039a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_826bfdf9-0fc3-4bcb-aca9-e5ca64cc3ef3" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_073de263-640d-4f5a-ada7-e32d30fe508d" xlink:to="loc_srt_MaximumMember_826bfdf9-0fc3-4bcb-aca9-e5ca64cc3ef3" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtScheduleofTermLoanFacilityDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtScheduleofTermLoanFacilityDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtScheduleofTermLoanFacilityDetails" xlink:type="extended" id="ib30ecd238c8842aeb5455a6867eb0a44_DebtScheduleofTermLoanFacilityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_6f27e38a-70b4-4db1-91fe-e812d320e148" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_23853047-2496-4968-bdc4-5ae7c5a446a6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_6f27e38a-70b4-4db1-91fe-e812d320e148" xlink:to="loc_us-gaap_LongTermDebt_23853047-2496-4968-bdc4-5ae7c5a446a6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_886e28be-9247-4287-ac4c-73b67cdb4701" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_6f27e38a-70b4-4db1-91fe-e812d320e148" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_886e28be-9247-4287-ac4c-73b67cdb4701" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_abe76ae2-dbec-4fef-b435-966511779eb2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_6f27e38a-70b4-4db1-91fe-e812d320e148" xlink:to="loc_us-gaap_DebtInstrumentTable_abe76ae2-dbec-4fef-b435-966511779eb2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_1c10c88b-71b7-40bd-909f-36ec436b9478" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_abe76ae2-dbec-4fef-b435-966511779eb2" xlink:to="loc_us-gaap_VariableRateAxis_1c10c88b-71b7-40bd-909f-36ec436b9478" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_1c10c88b-71b7-40bd-909f-36ec436b9478_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_1c10c88b-71b7-40bd-909f-36ec436b9478" xlink:to="loc_us-gaap_VariableRateDomain_1c10c88b-71b7-40bd-909f-36ec436b9478_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_db9bb53b-e309-4d34-b2e3-a8e3574a8df3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_1c10c88b-71b7-40bd-909f-36ec436b9478" xlink:to="loc_us-gaap_VariableRateDomain_db9bb53b-e309-4d34-b2e3-a8e3574a8df3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_3a193f0e-14be-4ff0-ae2f-80abebd2e24f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_db9bb53b-e309-4d34-b2e3-a8e3574a8df3" xlink:to="loc_us-gaap_BaseRateMember_3a193f0e-14be-4ff0-ae2f-80abebd2e24f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_bb8fd587-a6b2-44e8-a300-1011baf69a65" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_db9bb53b-e309-4d34-b2e3-a8e3574a8df3" xlink:to="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_bb8fd587-a6b2-44e8-a300-1011baf69a65" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_ada355f3-0a71-43ba-a7c5-0c21eefd43dd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_abe76ae2-dbec-4fef-b435-966511779eb2" xlink:to="loc_us-gaap_CreditFacilityAxis_ada355f3-0a71-43ba-a7c5-0c21eefd43dd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_ada355f3-0a71-43ba-a7c5-0c21eefd43dd_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_ada355f3-0a71-43ba-a7c5-0c21eefd43dd" xlink:to="loc_us-gaap_CreditFacilityDomain_ada355f3-0a71-43ba-a7c5-0c21eefd43dd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_0dc30bc9-15a3-4b1a-950b-e6e38afd2769" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_ada355f3-0a71-43ba-a7c5-0c21eefd43dd" xlink:to="loc_us-gaap_CreditFacilityDomain_0dc30bc9-15a3-4b1a-950b-e6e38afd2769" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_TermLoanFacilityMember_d7fec0d9-c23f-4f6a-b3c4-d3e19cbb2e31" xlink:href="xpo-20201231.xsd#xpo_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_0dc30bc9-15a3-4b1a-950b-e6e38afd2769" xlink:to="loc_xpo_TermLoanFacilityMember_d7fec0d9-c23f-4f6a-b3c4-d3e19cbb2e31" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_IncrementalLoanFacilityMember_7c13fa8c-9730-46d9-864d-206bf96a2afc" xlink:href="xpo-20201231.xsd#xpo_IncrementalLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_0dc30bc9-15a3-4b1a-950b-e6e38afd2769" xlink:to="loc_xpo_IncrementalLoanFacilityMember_7c13fa8c-9730-46d9-864d-206bf96a2afc" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtSeniorNotesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtSeniorNotesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtSeniorNotesDetails" xlink:type="extended" id="i63f2d6d33e8b48b59b3b9ae9f6e59d62_DebtSeniorNotesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_5f79d5fb-11a9-4351-8589-7883360854ab" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_LongTermDebt_5f79d5fb-11a9-4351-8589-7883360854ab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_326d43b4-1523-4b40-9f2b-24f0a9810129" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_326d43b4-1523-4b40-9f2b-24f0a9810129" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_6e83dea5-297e-431e-862b-fae79459afa1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_6e83dea5-297e-431e-862b-fae79459afa1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DebtInstrumentAmountIssuedAsPercentageOfPar_5e4d0a5f-4248-4429-b55c-123cd5dd75a4" xlink:href="xpo-20201231.xsd#xpo_DebtInstrumentAmountIssuedAsPercentageOfPar"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_xpo_DebtInstrumentAmountIssuedAsPercentageOfPar_5e4d0a5f-4248-4429-b55c-123cd5dd75a4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DebtIssuancePriceAsPercentageOfParValue_da8c7abf-39b9-468d-8896-744ceec6dbf7" xlink:href="xpo-20201231.xsd#xpo_DebtIssuancePriceAsPercentageOfParValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_xpo_DebtIssuancePriceAsPercentageOfParValue_da8c7abf-39b9-468d-8896-744ceec6dbf7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRepurchaseAmount_74f9e49e-0a8b-4416-8776-50ee19f50c3e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentRepurchaseAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_DebtInstrumentRepurchaseAmount_74f9e49e-0a8b-4416-8776-50ee19f50c3e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_a1383ac6-3dab-4676-8f66-584332f263ba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentRedemptionPricePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_a1383ac6-3dab-4676-8f66-584332f263ba" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_3c0bf163-538f-4281-afc3-0d3e09c898cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_3c0bf163-538f-4281-afc3-0d3e09c898cd" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_fb556c29-cad9-457e-839f-d42997efc4aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41256f67-f708-4be5-9f70-de91cae32bea" xlink:to="loc_us-gaap_DebtInstrumentTable_fb556c29-cad9-457e-839f-d42997efc4aa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_d38b8665-0cca-408c-b026-0055940dbf86" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_fb556c29-cad9-457e-839f-d42997efc4aa" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_d38b8665-0cca-408c-b026-0055940dbf86" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d38b8665-0cca-408c-b026-0055940dbf86_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d38b8665-0cca-408c-b026-0055940dbf86" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d38b8665-0cca-408c-b026-0055940dbf86_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_f968f75e-2124-4f2e-aee5-e646fb890408" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d38b8665-0cca-408c-b026-0055940dbf86" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_f968f75e-2124-4f2e-aee5-e646fb890408" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_4590aa24-96ff-4432-bffb-2d8ea117b558" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_f968f75e-2124-4f2e-aee5-e646fb890408" xlink:to="loc_us-gaap_SeniorNotesMember_4590aa24-96ff-4432-bffb-2d8ea117b558" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_9de64544-fbfb-46ae-bb7d-b311f40c34c7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_fb556c29-cad9-457e-839f-d42997efc4aa" xlink:to="loc_us-gaap_DebtInstrumentAxis_9de64544-fbfb-46ae-bb7d-b311f40c34c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_9de64544-fbfb-46ae-bb7d-b311f40c34c7_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_9de64544-fbfb-46ae-bb7d-b311f40c34c7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_9de64544-fbfb-46ae-bb7d-b311f40c34c7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_e65707d4-055c-4283-8e88-53674edbe9b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_9de64544-fbfb-46ae-bb7d-b311f40c34c7" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_e65707d4-055c-4283-8e88-53674edbe9b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2025Member_8b5a8041-12de-4d95-a990-2a8b607ce271" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2025Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e65707d4-055c-4283-8e88-53674edbe9b9" xlink:to="loc_xpo_SeniorNotesDue2025Member_8b5a8041-12de-4d95-a990-2a8b607ce271" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2024Member_6d2917e6-6231-4650-a157-fc20b031237b" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2024Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e65707d4-055c-4283-8e88-53674edbe9b9" xlink:to="loc_xpo_SeniorNotesDue2024Member_6d2917e6-6231-4650-a157-fc20b031237b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2022Member_004b4a35-e46b-4f9f-b6b3-d70dcd0ed08d" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2022Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_e65707d4-055c-4283-8e88-53674edbe9b9" xlink:to="loc_xpo_SeniorNotesDue2022Member_004b4a35-e46b-4f9f-b6b3-d70dcd0ed08d" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtSeniorDebenturesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtSeniorDebenturesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtSeniorDebenturesDetails" xlink:type="extended" id="i0de8b43ff6344093a5e41d19f245bb23_DebtSeniorDebenturesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_2959c21a-f9c3-467e-9ea2-b25c384ce527" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_bab57180-1121-4d4e-a394-d4d3988556b2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2959c21a-f9c3-467e-9ea2-b25c384ce527" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_bab57180-1121-4d4e-a394-d4d3988556b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt_ebd942ef-1cd0-4c03-b29f-6a7dc89eee77" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2959c21a-f9c3-467e-9ea2-b25c384ce527" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt_ebd942ef-1cd0-4c03-b29f-6a7dc89eee77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_4b35483d-02a6-42fd-9e63-33847ea167c0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2959c21a-f9c3-467e-9ea2-b25c384ce527" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_4b35483d-02a6-42fd-9e63-33847ea167c0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_0bbcf4a7-0fae-451b-9e2d-2da232fd63c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2959c21a-f9c3-467e-9ea2-b25c384ce527" xlink:to="loc_us-gaap_DebtInstrumentTable_0bbcf4a7-0fae-451b-9e2d-2da232fd63c4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_694d7e3a-cddb-44cc-a33c-665d592b96b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_0bbcf4a7-0fae-451b-9e2d-2da232fd63c4" xlink:to="loc_us-gaap_DebtInstrumentAxis_694d7e3a-cddb-44cc-a33c-665d592b96b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_694d7e3a-cddb-44cc-a33c-665d592b96b3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_694d7e3a-cddb-44cc-a33c-665d592b96b3" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_694d7e3a-cddb-44cc-a33c-665d592b96b3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_fbc342d4-b903-4c88-abdf-c62db209a19a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_694d7e3a-cddb-44cc-a33c-665d592b96b3" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_fbc342d4-b903-4c88-abdf-c62db209a19a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorDebenturesDue2034Member_90f64517-dc07-4a27-b19e-fda3940e7099" xlink:href="xpo-20201231.xsd#xpo_SeniorDebenturesDue2034Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_fbc342d4-b903-4c88-abdf-c62db209a19a" xlink:to="loc_xpo_SeniorDebenturesDue2034Member_90f64517-dc07-4a27-b19e-fda3940e7099" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtUnsecuredCreditFacilityDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtUnsecuredCreditFacilityDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtUnsecuredCreditFacilityDetails" xlink:type="extended" id="i75fd7b434ecf4e54a94ab58d40e74d2a_DebtUnsecuredCreditFacilityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_16ca6715-66a4-491b-9693-47d33282a67c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_938343e4-7a1c-413e-bdd4-4a3bccb52846" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_16ca6715-66a4-491b-9693-47d33282a67c" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_938343e4-7a1c-413e-bdd4-4a3bccb52846" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_7410cdf0-1679-460f-9e7b-ed72f9952e00" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_16ca6715-66a4-491b-9693-47d33282a67c" xlink:to="loc_us-gaap_ShortTermBorrowings_7410cdf0-1679-460f-9e7b-ed72f9952e00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_d1840174-57e3-4553-b178-9ad5f7eb0f9a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_16ca6715-66a4-491b-9693-47d33282a67c" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_d1840174-57e3-4553-b178-9ad5f7eb0f9a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_4c09dc52-ac00-4927-b876-039db5c0bb82" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_16ca6715-66a4-491b-9693-47d33282a67c" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_4c09dc52-ac00-4927-b876-039db5c0bb82" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_1fc8b8da-742d-42f6-930c-b218a4f9077b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_4c09dc52-ac00-4927-b876-039db5c0bb82" xlink:to="loc_us-gaap_CreditFacilityAxis_1fc8b8da-742d-42f6-930c-b218a4f9077b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_1fc8b8da-742d-42f6-930c-b218a4f9077b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_1fc8b8da-742d-42f6-930c-b218a4f9077b" xlink:to="loc_us-gaap_CreditFacilityDomain_1fc8b8da-742d-42f6-930c-b218a4f9077b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_f503a990-41d0-43f6-b2f6-7d9294f5da3e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_1fc8b8da-742d-42f6-930c-b218a4f9077b" xlink:to="loc_us-gaap_CreditFacilityDomain_f503a990-41d0-43f6-b2f6-7d9294f5da3e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_UnsecuredCreditFacilityMember_f4b3ee26-0898-4089-a9f1-c4673be4fb12" xlink:href="xpo-20201231.xsd#xpo_UnsecuredCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_f503a990-41d0-43f6-b2f6-7d9294f5da3e" xlink:to="loc_xpo_UnsecuredCreditFacilityMember_f4b3ee26-0898-4089-a9f1-c4673be4fb12" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/DebtAssetFinancingDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#DebtAssetFinancingDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/DebtAssetFinancingDetails" xlink:type="extended" id="i4d76dc051b794816bb678db8faba49b3_DebtAssetFinancingDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_72d7a021-ddba-4bdc-8717-f77aaac6ca60" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_22429b3e-654c-4380-ab97-0441b5a1f96c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_72d7a021-ddba-4bdc-8717-f77aaac6ca60" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_22429b3e-654c-4380-ab97-0441b5a1f96c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtWeightedAverageInterestRate_56f42c43-78b3-4831-a474-d6d9f17c0b98" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtWeightedAverageInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_72d7a021-ddba-4bdc-8717-f77aaac6ca60" xlink:to="loc_us-gaap_DebtWeightedAverageInterestRate_56f42c43-78b3-4831-a474-d6d9f17c0b98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_9f9486b6-5092-4d68-9b32-51780b98184f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_72d7a021-ddba-4bdc-8717-f77aaac6ca60" xlink:to="loc_us-gaap_DebtInstrumentTerm_9f9486b6-5092-4d68-9b32-51780b98184f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_8261a4e6-0dbd-4ab0-ba0b-13d5f044eaf7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_72d7a021-ddba-4bdc-8717-f77aaac6ca60" xlink:to="loc_us-gaap_DebtInstrumentTable_8261a4e6-0dbd-4ab0-ba0b-13d5f044eaf7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_8261a4e6-0dbd-4ab0-ba0b-13d5f044eaf7" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_04cbf23d-8056-4eab-9bb8-6770ddc444f4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_3a74fa3f-47b9-4ad4-ab55-e4eb5b9ab0db" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_04cbf23d-8056-4eab-9bb8-6770ddc444f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_a16bbe8b-e50a-44c0-b293-ccb5973632b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_04cbf23d-8056-4eab-9bb8-6770ddc444f4" xlink:to="loc_us-gaap_UnsecuredDebtMember_a16bbe8b-e50a-44c0-b293-ccb5973632b3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_4f2e2117-1337-40fd-8de1-42a48ed854cc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_8261a4e6-0dbd-4ab0-ba0b-13d5f044eaf7" xlink:to="loc_us-gaap_DebtInstrumentAxis_4f2e2117-1337-40fd-8de1-42a48ed854cc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_4f2e2117-1337-40fd-8de1-42a48ed854cc_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_4f2e2117-1337-40fd-8de1-42a48ed854cc" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_4f2e2117-1337-40fd-8de1-42a48ed854cc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_40d418e9-48cc-4bc8-b283-10d137fb0277" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_4f2e2117-1337-40fd-8de1-42a48ed854cc" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_40d418e9-48cc-4bc8-b283-10d137fb0277" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_AssetFinanceDebtMember_d05d778f-f16d-4c4e-8990-6eb4bbc64bd2" xlink:href="xpo-20201231.xsd#xpo_AssetFinanceDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_40d418e9-48cc-4bc8-b283-10d137fb0277" xlink:to="loc_xpo_AssetFinanceDebtMember_d05d778f-f16d-4c4e-8990-6eb4bbc64bd2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_8261a4e6-0dbd-4ab0-ba0b-13d5f044eaf7" xlink:to="loc_srt_RangeAxis_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba" xlink:to="loc_srt_RangeMember_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_e22c0fb7-c885-47fb-acfa-576977a484be" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_221cad10-a7c5-45a6-bcb8-f7ecc23f8cba" xlink:to="loc_srt_RangeMember_e22c0fb7-c885-47fb-acfa-576977a484be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_49045243-bac7-4cd2-bb58-374a30b5120a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_e22c0fb7-c885-47fb-acfa-576977a484be" xlink:to="loc_srt_MinimumMember_49045243-bac7-4cd2-bb58-374a30b5120a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_17b91f43-ea8b-47d6-a8aa-bb293a12bf70" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_e22c0fb7-c885-47fb-acfa-576977a484be" xlink:to="loc_srt_MaximumMember_17b91f43-ea8b-47d6-a8aa-bb293a12bf70" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlans" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlans"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlans" xlink:type="extended" id="ib899a959f62840f295611c2dabead8b2_EmployeeBenefitPlans"/>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansTables" xlink:type="extended" id="i1091c8c040794269a427a605cdf5d25e_EmployeeBenefitPlansTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansChangeinBenefitObligationsandFairValueofPlanAssetsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansChangeinBenefitObligationsandFairValueofPlanAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansChangeinBenefitObligationsandFairValueofPlanAssetsDetails" xlink:type="extended" id="ied805863828c4d93932e20da10b9a3d2_EmployeeBenefitPlansChangeinBenefitObligationsandFairValueofPlanAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e57e79ed-cce3-47b8-baea-06c3516d3428" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e57e79ed-cce3-47b8-baea-06c3516d3428" xlink:to="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligation_97851534-1336-4b58-b0b8-f180eda52178" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligation_97851534-1336-4b58-b0b8-f180eda52178" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_2fec87c1-8866-4504-8c9e-ee04f83a9bba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_2fec87c1-8866-4504-8c9e-ee04f83a9bba" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_98454475-b3a7-4ee0-83fa-2ff6a7e8e79d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanActuarialGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:to="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_98454475-b3a7-4ee0-83fa-2ff6a7e8e79d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid_f1911dfb-89ef-424d-8d7d-312220181137" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid_f1911dfb-89ef-424d-8d7d-312220181137" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanForeignCurrencyExchangeRateChangesBenefitObligation_e2af47de-4022-438b-bf6b-5a1ffb6dc892" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanForeignCurrencyExchangeRateChangesBenefitObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_0d1d2dea-0420-47e6-80e2-49657b485d36" xlink:to="loc_us-gaap_DefinedBenefitPlanForeignCurrencyExchangeRateChangesBenefitObligation_e2af47de-4022-438b-bf6b-5a1ffb6dc892" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligation_418d0270-91d2-4360-9d85-b662abe4093c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligation"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e57e79ed-cce3-47b8-baea-06c3516d3428" xlink:to="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_0916e478-db1b-4549-beec-5bf4fb45b64c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFairValueOfPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:to="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_0916e478-db1b-4549-beec-5bf4fb45b64c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActualReturnOnPlanAssets_74aff4fa-5ebb-4ce5-be35-feba18267e92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanActualReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:to="loc_us-gaap_DefinedBenefitPlanActualReturnOnPlanAssets_74aff4fa-5ebb-4ce5-be35-feba18267e92" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanContributionsByEmployer_5e9224e5-a81d-4b1a-adb7-e979daee6d59" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanContributionsByEmployer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:to="loc_us-gaap_DefinedBenefitPlanContributionsByEmployer_5e9224e5-a81d-4b1a-adb7-e979daee6d59" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPlanAssetsBenefitsPaid_a20ad2b0-4c65-4f2b-a558-5791137c8d9d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPlanAssetsBenefitsPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:to="loc_us-gaap_DefinedBenefitPlanPlanAssetsBenefitsPaid_a20ad2b0-4c65-4f2b-a558-5791137c8d9d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPlanAssetsForeignCurrencyTranslationGainLoss_fd67c400-b66b-4d8e-8ec4-9586cd185a5c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPlanAssetsForeignCurrencyTranslationGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInFairValueOfPlanAssetsRollForward_bec95de5-55dd-463c-9709-ee23ee5fef33" xlink:to="loc_us-gaap_DefinedBenefitPlanPlanAssetsForeignCurrencyTranslationGainLoss_fd67c400-b66b-4d8e-8ec4-9586cd185a5c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_8310df32-baf1-4bff-b5a4-42cb4d1167d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFairValueOfPlanAssets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4fb0fe0-f004-4a25-aaf4-17370b5e9c27" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e57e79ed-cce3-47b8-baea-06c3516d3428" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4fb0fe0-f004-4a25-aaf4-17370b5e9c27" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_65e422e1-199f-439e-a10d-b40b58d35059" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4fb0fe0-f004-4a25-aaf4-17370b5e9c27" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_65e422e1-199f-439e-a10d-b40b58d35059" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_65e422e1-199f-439e-a10d-b40b58d35059_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_65e422e1-199f-439e-a10d-b40b58d35059" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_65e422e1-199f-439e-a10d-b40b58d35059_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_9fbc860e-c0ac-4e0f-ac6e-3e149be9b848" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_65e422e1-199f-439e-a10d-b40b58d35059" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_9fbc860e-c0ac-4e0f-ac6e-3e149be9b848" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_8aeb452a-55f7-4d36-ae19-c45286ef08ee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_9fbc860e-c0ac-4e0f-ac6e-3e149be9b848" xlink:to="loc_us-gaap_DomesticPlanMember_8aeb452a-55f7-4d36-ae19-c45286ef08ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_2117913b-673b-42e0-868a-6a71fa2ee04b" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_8aeb452a-55f7-4d36-ae19-c45286ef08ee" xlink:to="loc_country_US_2117913b-673b-42e0-868a-6a71fa2ee04b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_631f3987-789b-4989-9b07-c23b3a97d0a3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_9fbc860e-c0ac-4e0f-ac6e-3e149be9b848" xlink:to="loc_us-gaap_ForeignPlanMember_631f3987-789b-4989-9b07-c23b3a97d0a3" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansFundedStatusofPlanDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansFundedStatusofPlanDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansFundedStatusofPlanDetails" xlink:type="extended" id="ib1f5af115c8249e081a7410d39da2cba_EmployeeBenefitPlansFundedStatusofPlanDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d2f11b72-5e9f-4039-8433-c1212825269f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_62eedf43-738c-4203-9d70-d45ec566323a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFundedStatusOfPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d2f11b72-5e9f-4039-8433-c1212825269f" xlink:to="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_62eedf43-738c-4203-9d70-d45ec566323a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d2f11b72-5e9f-4039-8433-c1212825269f" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssetsForPlanBenefitsNoncurrent_70695e7c-d4b6-4fb8-8377-99530ed128b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssetsForPlanBenefitsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:to="loc_us-gaap_DefinedBenefitPlanAssetsForPlanBenefitsNoncurrent_70695e7c-d4b6-4fb8-8377-99530ed128b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities_202810cb-da03-445a-82dd-844acf786a95" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:to="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities_202810cb-da03-445a-82dd-844acf786a95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_7302c865-3eb4-45d7-b0ee-3356b2f7d0e0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:to="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_7302c865-3eb4-45d7-b0ee-3356b2f7d0e0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet_4f137347-0d90-4ba6-ba4d-4dfb6bbc842c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_dc4f3a7d-d4c6-451a-b1df-15b927bb8434" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet_4f137347-0d90-4ba6-ba4d-4dfb6bbc842c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPlansWithBenefitObligationsInExcessOfPlanAssetsAbstract_69e340ff-7558-45e6-ac9a-2262f366a90c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPlansWithBenefitObligationsInExcessOfPlanAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d2f11b72-5e9f-4039-8433-c1212825269f" xlink:to="loc_us-gaap_DefinedBenefitPlanPlansWithBenefitObligationsInExcessOfPlanAssetsAbstract_69e340ff-7558-45e6-ac9a-2262f366a90c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPensionPlansWithAccumulatedBenefitObligationsInExcessOfPlanAssetsAggregateProjectedBenefitObligation_e0fa93fc-943b-4b47-bd4c-14b3f596398c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPensionPlansWithAccumulatedBenefitObligationsInExcessOfPlanAssetsAggregateProjectedBenefitObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanPlansWithBenefitObligationsInExcessOfPlanAssetsAbstract_69e340ff-7558-45e6-ac9a-2262f366a90c" xlink:to="loc_us-gaap_DefinedBenefitPlanPensionPlansWithAccumulatedBenefitObligationsInExcessOfPlanAssetsAggregateProjectedBenefitObligation_e0fa93fc-943b-4b47-bd4c-14b3f596398c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPensionPlanWithProjectedBenefitObligationInExcessOfPlanAssetsPlanAssets_cdfcb923-aebc-4055-8e25-df7659fb02d3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPensionPlanWithProjectedBenefitObligationInExcessOfPlanAssetsPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanPlansWithBenefitObligationsInExcessOfPlanAssetsAbstract_69e340ff-7558-45e6-ac9a-2262f366a90c" xlink:to="loc_us-gaap_DefinedBenefitPlanPensionPlanWithProjectedBenefitObligationInExcessOfPlanAssetsPlanAssets_cdfcb923-aebc-4055-8e25-df7659fb02d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4be17d7-771c-460a-802b-8023d8b14dd2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d2f11b72-5e9f-4039-8433-c1212825269f" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4be17d7-771c-460a-802b-8023d8b14dd2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusAxis_4329a284-98c4-460c-b503-18269822051a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4be17d7-771c-460a-802b-8023d8b14dd2" xlink:to="loc_us-gaap_RetirementPlanTaxStatusAxis_4329a284-98c4-460c-b503-18269822051a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_4329a284-98c4-460c-b503-18269822051a_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_4329a284-98c4-460c-b503-18269822051a" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_4329a284-98c4-460c-b503-18269822051a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_561e7435-36b6-44e0-bdd0-c425cdefa139" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_4329a284-98c4-460c-b503-18269822051a" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_561e7435-36b6-44e0-bdd0-c425cdefa139" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_QualifiedPlanMember_fd18fd30-8d0c-4f38-8cfb-2aef1b97b88a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_QualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_561e7435-36b6-44e0-bdd0-c425cdefa139" xlink:to="loc_us-gaap_QualifiedPlanMember_fd18fd30-8d0c-4f38-8cfb-2aef1b97b88a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_89593ec9-5a8a-487e-ab15-0b3087ba5195" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_e4be17d7-771c-460a-802b-8023d8b14dd2" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_89593ec9-5a8a-487e-ab15-0b3087ba5195" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_89593ec9-5a8a-487e-ab15-0b3087ba5195_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_89593ec9-5a8a-487e-ab15-0b3087ba5195" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_89593ec9-5a8a-487e-ab15-0b3087ba5195_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_5107a2bc-2d2e-464c-b1f4-62a45d6c8a0c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_89593ec9-5a8a-487e-ab15-0b3087ba5195" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_5107a2bc-2d2e-464c-b1f4-62a45d6c8a0c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_4fa92e8f-674d-45fa-a35a-1b1635ed8792" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_5107a2bc-2d2e-464c-b1f4-62a45d6c8a0c" xlink:to="loc_us-gaap_DomesticPlanMember_4fa92e8f-674d-45fa-a35a-1b1635ed8792" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_e1adecc4-3be1-47e4-adbc-944bc4a8f08b" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_4fa92e8f-674d-45fa-a35a-1b1635ed8792" xlink:to="loc_country_US_e1adecc4-3be1-47e4-adbc-944bc4a8f08b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_e00b0895-b73a-4259-9055-128ab8d07dd1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_5107a2bc-2d2e-464c-b1f4-62a45d6c8a0c" xlink:to="loc_us-gaap_ForeignPlanMember_e00b0895-b73a-4259-9055-128ab8d07dd1" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansAccumulatedOtherComprehensiveIncomeandNetPeriodicBenefitCostDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansAccumulatedOtherComprehensiveIncomeandNetPeriodicBenefitCostDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansAccumulatedOtherComprehensiveIncomeandNetPeriodicBenefitCostDetails" xlink:type="extended" id="id925bddb69f94136bcf9c94d62a79a3a_EmployeeBenefitPlansAccumulatedOtherComprehensiveIncomeandNetPeriodicBenefitCostDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract_c810163b-1fe0-4460-965b-2d8969d04c05" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:to="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract_c810163b-1fe0-4460-965b-2d8969d04c05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetGainsLossesAfterTax_598d937d-f319-41ff-8198-4a27395df137" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetGainsLossesAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract_c810163b-1fe0-4460-965b-2d8969d04c05" xlink:to="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetGainsLossesAfterTax_598d937d-f319-41ff-8198-4a27395df137" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetPriorServiceCostCreditAfterTax_d35e8dcc-654b-435c-9aab-b5b4e3c8401d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetPriorServiceCostCreditAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract_c810163b-1fe0-4460-965b-2d8969d04c05" xlink:to="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeNetPriorServiceCostCreditAfterTax_d35e8dcc-654b-435c-9aab-b5b4e3c8401d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossDefinedBenefitPensionAndOtherPostretirementPlansNetOfTax_4639a185-1e35-456f-bb2b-a1bfaffb6f5d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossDefinedBenefitPensionAndOtherPostretirementPlansNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAccumulatedOtherComprehensiveIncomeLossAfterTaxAbstract_c810163b-1fe0-4460-965b-2d8969d04c05" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossDefinedBenefitPensionAndOtherPostretirementPlansNetOfTax_4639a185-1e35-456f-bb2b-a1bfaffb6f5d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_75e7903d-482a-4d57-92c8-e670dcfddadd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_75e7903d-482a-4d57-92c8-e670dcfddadd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_12aa318b-17c8-4f0d-88b2-4fdbbe822e62" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_12aa318b-17c8-4f0d-88b2-4fdbbe822e62" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_ceb8721a-af8e-4b58-b0b1-251897ba3bc2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_ceb8721a-af8e-4b58-b0b1-251897ba3bc2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_9ac217f9-9d2d-4dec-9a0f-ca555151f98e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_fc0ee266-4cbf-4c9c-894f-0e4782368435" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_9ac217f9-9d2d-4dec-9a0f-ca555151f98e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeLossNetGainLossBeforeTax_58035852-fa89-4576-bdc4-8a983ddba652" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeLossNetGainLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeLossNetGainLossBeforeTax_58035852-fa89-4576-bdc4-8a983ddba652" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlanNetPriorServiceCostCreditArisingDuringPeriodBeforeTax_c064b79c-2557-4357-a9c2-903934e67dfa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeDefinedBenefitPlanNetPriorServiceCostCreditArisingDuringPeriodBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlanNetPriorServiceCostCreditArisingDuringPeriodBeforeTax_c064b79c-2557-4357-a9c2-903934e67dfa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossAmortizationAdjustmentFromAOCIPensionAndOtherPostretirementBenefitPlansForNetPriorServiceCostCreditBeforeTax_07a41f0a-e52f-44c0-9481-cebd140398c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossAmortizationAdjustmentFromAOCIPensionAndOtherPostretirementBenefitPlansForNetPriorServiceCostCreditBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossAmortizationAdjustmentFromAOCIPensionAndOtherPostretirementBenefitPlansForNetPriorServiceCostCreditBeforeTax_07a41f0a-e52f-44c0-9481-cebd140398c3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeNetPriorServiceCostCreditBeforeTax_deab9be5-815e-4e14-9a61-659e54ef3010" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeNetPriorServiceCostCreditBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeAbstract_d98753f4-ef05-46d0-9256-6a609d1c924e" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInOtherComprehensiveIncomeNetPriorServiceCostCreditBeforeTax_deab9be5-815e-4e14-9a61-659e54ef3010" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract_81a4ed4a-93e3-4321-9d47-4782f6949b14" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract_81a4ed4a-93e3-4321-9d47-4782f6949b14" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate_1ffc26d5-fe70-4ae0-b9c8-7fd674cda631" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract_81a4ed4a-93e3-4321-9d47-4782f6949b14" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate_1ffc26d5-fe70-4ae0-b9c8-7fd674cda631" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate_017b55e9-8b91-412f-bb20-8c4d109d5d60" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract_81a4ed4a-93e3-4321-9d47-4782f6949b14" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate_017b55e9-8b91-412f-bb20-8c4d109d5d60" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets_85245572-59d0-4124-9d9a-52b0abf412f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedInCalculationsAbstract_81a4ed4a-93e3-4321-9d47-4782f6949b14" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets_85245572-59d0-4124-9d9a-52b0abf412f5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_bfcf555f-047b-439d-ac35-bf6747ff2108" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusAxis_394124bc-7aa0-4b78-b2e0-28f3da0d3406" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:to="loc_us-gaap_RetirementPlanTaxStatusAxis_394124bc-7aa0-4b78-b2e0-28f3da0d3406" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_394124bc-7aa0-4b78-b2e0-28f3da0d3406_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_394124bc-7aa0-4b78-b2e0-28f3da0d3406" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_394124bc-7aa0-4b78-b2e0-28f3da0d3406_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_c8fee1dd-0361-48ce-827a-1f5310a34851" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_394124bc-7aa0-4b78-b2e0-28f3da0d3406" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_c8fee1dd-0361-48ce-827a-1f5310a34851" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_QualifiedPlanMember_d9c28ece-876f-4c9f-8704-c1c11309b01c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_QualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_c8fee1dd-0361-48ce-827a-1f5310a34851" xlink:to="loc_us-gaap_QualifiedPlanMember_d9c28ece-876f-4c9f-8704-c1c11309b01c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonqualifiedPlanMember_f89a5663-b8b0-447a-beeb-a6282eb2a78b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonqualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_c8fee1dd-0361-48ce-827a-1f5310a34851" xlink:to="loc_us-gaap_NonqualifiedPlanMember_f89a5663-b8b0-447a-beeb-a6282eb2a78b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_0274c958-e41c-4d21-8d6c-891edbcd820c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_0274c958-e41c-4d21-8d6c-891edbcd820c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_0274c958-e41c-4d21-8d6c-891edbcd820c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_0274c958-e41c-4d21-8d6c-891edbcd820c" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_0274c958-e41c-4d21-8d6c-891edbcd820c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_059c1611-a0ed-42f6-891e-258e249c12a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_0274c958-e41c-4d21-8d6c-891edbcd820c" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_059c1611-a0ed-42f6-891e-258e249c12a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_726c6c85-598b-460d-aa6f-185285c71d4d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_059c1611-a0ed-42f6-891e-258e249c12a7" xlink:to="loc_us-gaap_DomesticPlanMember_726c6c85-598b-460d-aa6f-185285c71d4d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_6c465e7f-fbc4-4b90-a9d9-e85c939a3bd0" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_726c6c85-598b-460d-aa6f-185285c71d4d" xlink:to="loc_country_US_6c465e7f-fbc4-4b90-a9d9-e85c939a3bd0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_341108d1-49e2-4ab9-9cfd-c0760111e553" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_059c1611-a0ed-42f6-891e-258e249c12a7" xlink:to="loc_us-gaap_ForeignPlanMember_341108d1-49e2-4ab9-9cfd-c0760111e553" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_6eecd3a7-2a7f-4e75-8084-6fa1147c9647" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:to="loc_srt_RangeAxis_6eecd3a7-2a7f-4e75-8084-6fa1147c9647" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_6eecd3a7-2a7f-4e75-8084-6fa1147c9647_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_6eecd3a7-2a7f-4e75-8084-6fa1147c9647" xlink:to="loc_srt_RangeMember_6eecd3a7-2a7f-4e75-8084-6fa1147c9647_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9b1ced79-28e2-4b1c-837b-37ff66aa1e62" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_6eecd3a7-2a7f-4e75-8084-6fa1147c9647" xlink:to="loc_srt_RangeMember_9b1ced79-28e2-4b1c-837b-37ff66aa1e62" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_1c9fa299-023b-4e66-98f7-44e6317a7140" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9b1ced79-28e2-4b1c-837b-37ff66aa1e62" xlink:to="loc_srt_MinimumMember_1c9fa299-023b-4e66-98f7-44e6317a7140" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_19423b03-3fb5-4771-a9b1-1cb2108be61f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9b1ced79-28e2-4b1c-837b-37ff66aa1e62" xlink:to="loc_srt_MaximumMember_19423b03-3fb5-4771-a9b1-1cb2108be61f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_99df0d4f-b558-4ad3-b5ab-0da628c99765" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_f39a50e6-9b73-4d54-8d79-bbd200eba066" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_99df0d4f-b558-4ad3-b5ab-0da628c99765" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_99df0d4f-b558-4ad3-b5ab-0da628c99765_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_99df0d4f-b558-4ad3-b5ab-0da628c99765" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_99df0d4f-b558-4ad3-b5ab-0da628c99765_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_07876052-34d4-4d0f-886f-97fc84badd7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_99df0d4f-b558-4ad3-b5ab-0da628c99765" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_07876052-34d4-4d0f-886f-97fc84badd7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_d3a9e891-802f-401b-9440-c514cbd97db9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPostretirementHealthCoverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_07876052-34d4-4d0f-886f-97fc84badd7a" xlink:to="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_d3a9e891-802f-401b-9440-c514cbd97db9" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansExpectedPaymentsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansExpectedPaymentsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansExpectedPaymentsDetails" xlink:type="extended" id="i71bace0792b84bcdbffc709438afb9a1_EmployeeBenefitPlansExpectedPaymentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_3b6f0e27-ffee-4a5d-9869-13d8d677ea56" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_3b6f0e27-ffee-4a5d-9869-13d8d677ea56" xlink:to="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsNextTwelveMonths_b0d4561e-6001-49b6-893d-ac4baed4c97c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsNextTwelveMonths_b0d4561e-6001-49b6-893d-ac4baed4c97c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearTwo_94474ecb-3b60-4d19-9109-040d53aaef3d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearTwo_94474ecb-3b60-4d19-9109-040d53aaef3d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearThree_660179dc-9a72-4666-bf09-294bd59c0f76" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearThree_660179dc-9a72-4666-bf09-294bd59c0f76" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFour_72103690-67e9-4f53-bc86-82e35f1233e5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFour_72103690-67e9-4f53-bc86-82e35f1233e5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFive_dc7a1aa0-453d-4d79-839a-dc91fa4ad6d9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsYearFive_dc7a1aa0-453d-4d79-839a-dc91fa4ad6d9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsFiveFiscalYearsThereafter_e9a08bf8-3149-4fca-8a62-3dc921f100d5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsFiveFiscalYearsThereafter"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanEstimatedFutureBenefitPaymentsAbstract_5ce64828-a1f9-4cf6-9506-016de580237a" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureBenefitPaymentsFiveFiscalYearsThereafter_e9a08bf8-3149-4fca-8a62-3dc921f100d5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_37efc3c1-48ed-478e-933d-673a2caf7c1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_3b6f0e27-ffee-4a5d-9869-13d8d677ea56" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_37efc3c1-48ed-478e-933d-673a2caf7c1b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_d4c541b1-9917-4963-ad02-1d1c711b73f4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_37efc3c1-48ed-478e-933d-673a2caf7c1b" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_d4c541b1-9917-4963-ad02-1d1c711b73f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_d4c541b1-9917-4963-ad02-1d1c711b73f4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_d4c541b1-9917-4963-ad02-1d1c711b73f4" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_d4c541b1-9917-4963-ad02-1d1c711b73f4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_4c331a8c-4879-40dc-bc4f-b7e6472c7742" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_d4c541b1-9917-4963-ad02-1d1c711b73f4" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_4c331a8c-4879-40dc-bc4f-b7e6472c7742" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_8574b047-2509-454c-b129-3cdd85e7bd70" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_4c331a8c-4879-40dc-bc4f-b7e6472c7742" xlink:to="loc_us-gaap_DomesticPlanMember_8574b047-2509-454c-b129-3cdd85e7bd70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_d967ead2-83f8-4dfa-a5bc-878ed16d3edd" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_8574b047-2509-454c-b129-3cdd85e7bd70" xlink:to="loc_country_US_d967ead2-83f8-4dfa-a5bc-878ed16d3edd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_44e0c935-eed1-48e4-afa0-ffdecd888383" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_4c331a8c-4879-40dc-bc4f-b7e6472c7742" xlink:to="loc_us-gaap_ForeignPlanMember_44e0c935-eed1-48e4-afa0-ffdecd888383" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_43a96388-b56a-49ac-ba1e-afb671b4413b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_37efc3c1-48ed-478e-933d-673a2caf7c1b" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_43a96388-b56a-49ac-ba1e-afb671b4413b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_43a96388-b56a-49ac-ba1e-afb671b4413b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_43a96388-b56a-49ac-ba1e-afb671b4413b" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_43a96388-b56a-49ac-ba1e-afb671b4413b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_8c1cfa7b-5545-48f8-aaa2-2357b92744be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_43a96388-b56a-49ac-ba1e-afb671b4413b" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_8c1cfa7b-5545-48f8-aaa2-2357b92744be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_8b5779f7-d940-49e7-855c-ada33f8c3eda" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPostretirementHealthCoverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_8c1cfa7b-5545-48f8-aaa2-2357b92744be" xlink:to="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_8b5779f7-d940-49e7-855c-ada33f8c3eda" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansNarrativeDetails" xlink:type="extended" id="ie91dc778b098425d9b34eae5fbae659b_EmployeeBenefitPlansNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_db950432-66b1-4254-9264-c5a9555ed66c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFundedStatusOfPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_db950432-66b1-4254-9264-c5a9555ed66c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPlanAssetsTargetAllocationPercentage_fa7d7198-ccc6-402a-bc17-a3db58138cc1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPlanAssetsTargetAllocationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedBenefitPlanPlanAssetsTargetAllocationPercentage_fa7d7198-ccc6-402a-bc17-a3db58138cc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DefinedBenefitPlanPlanAssetsTargetToHedgeProjectedLiabilitiesPercentage_97533d9d-639c-4f3b-af85-e800732060d4" xlink:href="xpo-20201231.xsd#xpo_DefinedBenefitPlanPlanAssetsTargetToHedgeProjectedLiabilitiesPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_xpo_DefinedBenefitPlanPlanAssetsTargetToHedgeProjectedLiabilitiesPercentage_97533d9d-639c-4f3b-af85-e800732060d4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanPlanAssetsInvestmentWithinPlanAssetCategoryPercentage_39114d2a-4309-4e8f-9e8f-d7500b8074cb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanPlanAssetsInvestmentWithinPlanAssetCategoryPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedBenefitPlanPlanAssetsInvestmentWithinPlanAssetCategoryPercentage_39114d2a-4309-4e8f-9e8f-d7500b8074cb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets_67947644-4ae0-4853-a8d4-3b5d157a6a90" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostExpectedLongTermReturnOnAssets_67947644-4ae0-4853-a8d4-3b5d157a6a90" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DefinedBenefitPlanActuarialLiabilityInterestRateSensitivityHedgedPercentage_1731f99f-f9e5-4662-a079-a4dece11eca3" xlink:href="xpo-20201231.xsd#xpo_DefinedBenefitPlanActuarialLiabilityInterestRateSensitivityHedgedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_xpo_DefinedBenefitPlanActuarialLiabilityInterestRateSensitivityHedgedPercentage_1731f99f-f9e5-4662-a079-a4dece11eca3" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DefinedBenefitPlanActuarialLiabilityInflationRateSensitivityHedgedPercentage_65f7c466-679b-4e7a-8651-f0ecca8b4dfb" xlink:href="xpo-20201231.xsd#xpo_DefinedBenefitPlanActuarialLiabilityInflationRateSensitivityHedgedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_xpo_DefinedBenefitPlanActuarialLiabilityInflationRateSensitivityHedgedPercentage_65f7c466-679b-4e7a-8651-f0ecca8b4dfb" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedFutureEmployerContributionsNextFiscalYear_bcec692e-2375-4431-ba96-948892599c40" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanExpectedFutureEmployerContributionsNextFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedFutureEmployerContributionsNextFiscalYear_bcec692e-2375-4431-ba96-948892599c40" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedContributionPlanCostRecognized_dea0cb4a-d22f-4ecb-adad-01a430efa50f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedContributionPlanCostRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_DefinedContributionPlanCostRecognized_dea0cb4a-d22f-4ecb-adad-01a430efa50f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_cdf91ffb-ec4c-497a-b47a-b8fa2ae4bf9c" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_23ec0fd6-247b-4c8d-8a08-01575a5a63e9" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:to="loc_srt_RangeAxis_23ec0fd6-247b-4c8d-8a08-01575a5a63e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_23ec0fd6-247b-4c8d-8a08-01575a5a63e9_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_23ec0fd6-247b-4c8d-8a08-01575a5a63e9" xlink:to="loc_srt_RangeMember_23ec0fd6-247b-4c8d-8a08-01575a5a63e9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_cafffe1d-9f35-4403-819a-0855a4c7b43a" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_23ec0fd6-247b-4c8d-8a08-01575a5a63e9" xlink:to="loc_srt_RangeMember_cafffe1d-9f35-4403-819a-0855a4c7b43a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_909e8491-e54f-4794-a553-134996d07b20" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cafffe1d-9f35-4403-819a-0855a4c7b43a" xlink:to="loc_srt_MinimumMember_909e8491-e54f-4794-a553-134996d07b20" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_3ed9613f-814b-4c66-9e74-5ee86098dc46" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_cafffe1d-9f35-4403-819a-0855a4c7b43a" xlink:to="loc_srt_MaximumMember_3ed9613f-814b-4c66-9e74-5ee86098dc46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusAxis_dcc9f972-d139-4e65-8d22-84f19dad7987" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:to="loc_us-gaap_RetirementPlanTaxStatusAxis_dcc9f972-d139-4e65-8d22-84f19dad7987" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_dcc9f972-d139-4e65-8d22-84f19dad7987_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_dcc9f972-d139-4e65-8d22-84f19dad7987" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_dcc9f972-d139-4e65-8d22-84f19dad7987_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_a056669f-1ff2-474c-b943-04c65e461887" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_dcc9f972-d139-4e65-8d22-84f19dad7987" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_a056669f-1ff2-474c-b943-04c65e461887" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_QualifiedPlanMember_b3510be9-e4d2-4fc1-90ab-bef0f9240ceb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_QualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_a056669f-1ff2-474c-b943-04c65e461887" xlink:to="loc_us-gaap_QualifiedPlanMember_b3510be9-e4d2-4fc1-90ab-bef0f9240ceb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonqualifiedPlanMember_71144c50-4e2e-403a-822d-2a8f556b3ef9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NonqualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_a056669f-1ff2-474c-b943-04c65e461887" xlink:to="loc_us-gaap_NonqualifiedPlanMember_71144c50-4e2e-403a-822d-2a8f556b3ef9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_5aa6f751-09f0-40d1-a330-08450da9c930" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_5aa6f751-09f0-40d1-a330-08450da9c930" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_5aa6f751-09f0-40d1-a330-08450da9c930_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_5aa6f751-09f0-40d1-a330-08450da9c930" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_5aa6f751-09f0-40d1-a330-08450da9c930_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_0c59013e-39af-4bbd-8708-22aeae78bde0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_5aa6f751-09f0-40d1-a330-08450da9c930" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_0c59013e-39af-4bbd-8708-22aeae78bde0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_6f5d64da-2bd7-4269-85e0-611b7260d6cd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_0c59013e-39af-4bbd-8708-22aeae78bde0" xlink:to="loc_us-gaap_DomesticPlanMember_6f5d64da-2bd7-4269-85e0-611b7260d6cd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_e52e5e08-31ee-402c-9ab5-360ce2efb3c2" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_6f5d64da-2bd7-4269-85e0-611b7260d6cd" xlink:to="loc_country_US_e52e5e08-31ee-402c-9ab5-360ce2efb3c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_2a12a435-5c71-44af-bd9c-59c012f6b49e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_0c59013e-39af-4bbd-8708-22aeae78bde0" xlink:to="loc_us-gaap_ForeignPlanMember_2a12a435-5c71-44af-bd9c-59c012f6b49e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_6812112a-efe2-4872-9942-cf1ca7768e5a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_6812112a-efe2-4872-9942-cf1ca7768e5a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_6812112a-efe2-4872-9942-cf1ca7768e5a_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_6812112a-efe2-4872-9942-cf1ca7768e5a" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_6812112a-efe2-4872-9942-cf1ca7768e5a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_2703fb01-49a3-468b-b426-d4ecbe29d35c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_6812112a-efe2-4872-9942-cf1ca7768e5a" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_2703fb01-49a3-468b-b426-d4ecbe29d35c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_QualifiedPlanMember_f57128e4-bf3d-430f-a62b-573a517498a8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_QualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_2703fb01-49a3-468b-b426-d4ecbe29d35c" xlink:to="loc_us-gaap_QualifiedPlanMember_f57128e4-bf3d-430f-a62b-573a517498a8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_3d177461-5351-43ae-a446-a388476cd64e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0b23037a-7c4a-4a91-bd69-6bf8f5758cbb" xlink:to="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_3d177461-5351-43ae-a446-a388476cd64e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanAssetCategoriesDomain_3d177461-5351-43ae-a446-a388476cd64e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanAssetCategoriesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_3d177461-5351-43ae-a446-a388476cd64e" xlink:to="loc_us-gaap_PlanAssetCategoriesDomain_3d177461-5351-43ae-a446-a388476cd64e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanAssetCategoriesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_3d177461-5351-43ae-a446-a388476cd64e" xlink:to="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FixedIncomeSecuritiesMember_483b6dae-371c-4298-a391-df0b08b73743" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FixedIncomeSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_us-gaap_FixedIncomeSecuritiesMember_483b6dae-371c-4298-a391-df0b08b73743" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesMember_566b7f48-df98-4429-9804-d855a0dd0320" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesMember_566b7f48-df98-4429-9804-d855a0dd0320" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsMember_7846b6a2-a565-4bc1-9d7d-21b867463bcf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsMember_7846b6a2-a565-4bc1-9d7d-21b867463bcf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember_2a26aa6a-fa2a-4c71-86d1-69d6d32645c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember_2a26aa6a-fa2a-4c71-86d1-69d6d32645c6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_MatchingAssetsMember_e6624679-fa23-465f-83cf-1290ffc40e94" xlink:href="xpo-20201231.xsd#xpo_MatchingAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_xpo_MatchingAssetsMember_e6624679-fa23-465f-83cf-1290ffc40e94" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_GrowthAssetsMember_00a14634-56a4-4bfc-9ee4-5a453350ed84" xlink:href="xpo-20201231.xsd#xpo_GrowthAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_c93f9313-92da-430f-a72b-79b12702b5c5" xlink:to="loc_xpo_GrowthAssetsMember_00a14634-56a4-4bfc-9ee4-5a453350ed84" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansFairValueofInvestmentCategoriesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansFairValueofInvestmentCategoriesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansFairValueofInvestmentCategoriesDetails" xlink:type="extended" id="i40c339931317401399e38b36286713b9_EmployeeBenefitPlansFairValueofInvestmentCategoriesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c884708c-ae7d-475b-a422-a3888a66abd9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_700677ae-b534-4e60-b1df-253d9534123c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFairValueOfPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c884708c-ae7d-475b-a422-a3888a66abd9" xlink:to="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_700677ae-b534-4e60-b1df-253d9534123c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_DefinedBenefitPlanAlternativeInvestmentsFairValueOfPlanAssets_925414b3-ebc0-4395-b8be-c3c8d8c378ae" xlink:href="xpo-20201231.xsd#xpo_DefinedBenefitPlanAlternativeInvestmentsFairValueOfPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c884708c-ae7d-475b-a422-a3888a66abd9" xlink:to="loc_xpo_DefinedBenefitPlanAlternativeInvestmentsFairValueOfPlanAssets_925414b3-ebc0-4395-b8be-c3c8d8c378ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanWeightedAverageAssetAllocations_2cf35263-2165-42af-b6b7-26103b1514c4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanWeightedAverageAssetAllocations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c884708c-ae7d-475b-a422-a3888a66abd9" xlink:to="loc_us-gaap_DefinedBenefitPlanWeightedAverageAssetAllocations_2cf35263-2165-42af-b6b7-26103b1514c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_c884708c-ae7d-475b-a422-a3888a66abd9" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusAxis_9d55fa87-f654-49eb-9b2a-c4f773d9dcba" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:to="loc_us-gaap_RetirementPlanTaxStatusAxis_9d55fa87-f654-49eb-9b2a-c4f773d9dcba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_9d55fa87-f654-49eb-9b2a-c4f773d9dcba_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_9d55fa87-f654-49eb-9b2a-c4f773d9dcba" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_9d55fa87-f654-49eb-9b2a-c4f773d9dcba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTaxStatusDomain_8c7eb520-c23c-4133-8072-4522dace0740" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTaxStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTaxStatusAxis_9d55fa87-f654-49eb-9b2a-c4f773d9dcba" xlink:to="loc_us-gaap_RetirementPlanTaxStatusDomain_8c7eb520-c23c-4133-8072-4522dace0740" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_QualifiedPlanMember_1ac4dd9f-7e6e-44d5-aadf-5acd9ab88f28" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_QualifiedPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTaxStatusDomain_8c7eb520-c23c-4133-8072-4522dace0740" xlink:to="loc_us-gaap_QualifiedPlanMember_1ac4dd9f-7e6e-44d5-aadf-5acd9ab88f28" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationAxis_959a62c2-6d93-4f42-baa2-4d7949d6097c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationAxis_959a62c2-6d93-4f42-baa2-4d7949d6097c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_959a62c2-6d93-4f42-baa2-4d7949d6097c_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_959a62c2-6d93-4f42-baa2-4d7949d6097c" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_959a62c2-6d93-4f42-baa2-4d7949d6097c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanSponsorLocationDomain_926c3aa4-c2b9-4716-8673-e744ce1e7968" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanSponsorLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationAxis_959a62c2-6d93-4f42-baa2-4d7949d6097c" xlink:to="loc_us-gaap_RetirementPlanSponsorLocationDomain_926c3aa4-c2b9-4716-8673-e744ce1e7968" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticPlanMember_7518636e-f0fe-4e7c-851e-0a8bcc9c54cf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_926c3aa4-c2b9-4716-8673-e744ce1e7968" xlink:to="loc_us-gaap_DomesticPlanMember_7518636e-f0fe-4e7c-851e-0a8bcc9c54cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_d1cde183-9ecd-4754-ad03-fd5d37b7b382" xlink:href="https://xbrl.sec.gov/country/2020/country-2020-01-31.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DomesticPlanMember_7518636e-f0fe-4e7c-851e-0a8bcc9c54cf" xlink:to="loc_country_US_d1cde183-9ecd-4754-ad03-fd5d37b7b382" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignPlanMember_76687f82-f4af-47db-8245-8130f7717139" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanSponsorLocationDomain_926c3aa4-c2b9-4716-8673-e744ce1e7968" xlink:to="loc_us-gaap_ForeignPlanMember_76687f82-f4af-47db-8245-8130f7717139" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_99a4c7a7-1c39-4bd8-99cb-48d12678204e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:to="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_99a4c7a7-1c39-4bd8-99cb-48d12678204e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanAssetCategoriesDomain_99a4c7a7-1c39-4bd8-99cb-48d12678204e_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanAssetCategoriesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_99a4c7a7-1c39-4bd8-99cb-48d12678204e" xlink:to="loc_us-gaap_PlanAssetCategoriesDomain_99a4c7a7-1c39-4bd8-99cb-48d12678204e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanAssetCategoriesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DefinedBenefitPlanByPlanAssetCategoriesAxis_99a4c7a7-1c39-4bd8-99cb-48d12678204e" xlink:to="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanCashAndCashEquivalentsMember_ba56d0b9-3c38-4a0e-9563-76b4fc15d9fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_DefinedBenefitPlanCashAndCashEquivalentsMember_ba56d0b9-3c38-4a0e-9563-76b4fc15d9fd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsLargeCapMember_90c5fcbd-d55d-4fcb-964d-4f78c3413418" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesUsLargeCapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsLargeCapMember_90c5fcbd-d55d-4fcb-964d-4f78c3413418" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsSmallCapMember_ae918ceb-9a07-43f4-9fbb-01e0229ecce2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesUsSmallCapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesUsSmallCapMember_ae918ceb-9a07-43f4-9fbb-01e0229ecce2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember_4b8d889b-c291-4d90-a718-8360175e4abd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_DefinedBenefitPlanEquitySecuritiesNonUsMember_4b8d889b-c291-4d90-a718-8360175e4abd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FixedIncomeSecuritiesMember_60a542bf-2115-4a6f-acdd-bd123423b6c6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FixedIncomeSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_FixedIncomeSecuritiesMember_60a542bf-2115-4a6f-acdd-bd123423b6c6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDerivativeMember_b358b862-568a-4f3f-b33e-81b981fce2e2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDerivativeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanAssetCategoriesDomain_3a91c351-4a11-4607-9e06-15a62af2453f" xlink:to="loc_us-gaap_DefinedBenefitPlanDerivativeMember_b358b862-568a-4f3f-b33e-81b981fce2e2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3582fbf8-4d00-45ae-b472-9f05781ecb39" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_29a807cc-71ef-4f85-a43b-b041d375f182" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3582fbf8-4d00-45ae-b472-9f05781ecb39" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3582fbf8-4d00-45ae-b472-9f05781ecb39_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3582fbf8-4d00-45ae-b472-9f05781ecb39" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3582fbf8-4d00-45ae-b472-9f05781ecb39_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_feeb599f-af6f-4a27-9715-53fb9810662a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3582fbf8-4d00-45ae-b472-9f05781ecb39" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_feeb599f-af6f-4a27-9715-53fb9810662a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_60f42d32-b7f9-45ac-9708-119e03172e8b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_feeb599f-af6f-4a27-9715-53fb9810662a" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_60f42d32-b7f9-45ac-9708-119e03172e8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_84b81a3c-46e1-44a2-864c-4815fb4bc7be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_feeb599f-af6f-4a27-9715-53fb9810662a" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_84b81a3c-46e1-44a2-864c-4815fb4bc7be" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/EmployeeBenefitPlansBenefitObligationsandFundedStatusforMedicalPlanDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EmployeeBenefitPlansBenefitObligationsandFundedStatusforMedicalPlanDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EmployeeBenefitPlansBenefitObligationsandFundedStatusforMedicalPlanDetails" xlink:type="extended" id="ic0ee443f6b994780a0d574462afc4b1f_EmployeeBenefitPlansBenefitObligationsandFundedStatusforMedicalPlanDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligation_11f24ee0-f07e-4f3b-9c4c-132b90232b93" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligation_11f24ee0-f07e-4f3b-9c4c-132b90232b93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_2baf545f-cb46-4865-9239-b87aec42f48f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_2baf545f-cb46-4865-9239-b87aec42f48f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_705c7889-0457-49bb-9b37-3998dd95f1e6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanActuarialGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:to="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_705c7889-0457-49bb-9b37-3998dd95f1e6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligationContributionsByPlanParticipant_c7511b9f-2529-4255-b77e-7bace43a05f5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligationContributionsByPlanParticipant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligationContributionsByPlanParticipant_c7511b9f-2529-4255-b77e-7bace43a05f5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid_b53249fb-5831-4560-9c6e-7ea294d271dc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanChangeInBenefitObligationRollForward_18cedc86-ab66-4beb-b790-44c437c2cb93" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligationBenefitsPaid_b53249fb-5831-4560-9c6e-7ea294d271dc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligation_709cb540-3f89-4d97-bb79-e2e6aa4ea49b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanBenefitObligation"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_3246973a-dc8b-4586-8b4c-9ceab14a4dd0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanFundedStatusOfPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_DefinedBenefitPlanFundedStatusOfPlan_3246973a-dc8b-4586-8b4c-9ceab14a4dd0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_e9989004-9094-44f7-acb8-e166df0f37c2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_e9989004-9094-44f7-acb8-e166df0f37c2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities_ce66e32c-5cbb-4488-8466-1bf3cb10c0ef" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_e9989004-9094-44f7-acb8-e166df0f37c2" xlink:to="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansCurrentLiabilities_ce66e32c-5cbb-4488-8466-1bf3cb10c0ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_9defe160-80be-4319-a815-2f1a4522925d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_e9989004-9094-44f7-acb8-e166df0f37c2" xlink:to="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_9defe160-80be-4319-a815-2f1a4522925d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet_65bdcef6-8a0c-4bfa-ae17-ad3146268bf1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheetAbstract_e9989004-9094-44f7-acb8-e166df0f37c2" xlink:to="loc_us-gaap_DefinedBenefitPlanAmountsRecognizedInBalanceSheet_65bdcef6-8a0c-4bfa-ae17-ad3146268bf1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate_6546c3dd-0e7e-40d2-8ff6-3005fb78cee9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingBenefitObligationDiscountRate_6546c3dd-0e7e-40d2-8ff6-3005fb78cee9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate_d97f5bcc-435d-4acc-9dc5-11678b91a478" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_DefinedBenefitPlanAssumptionsUsedCalculatingNetPeriodicBenefitCostDiscountRate_d97f5bcc-435d-4acc-9dc5-11678b91a478" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_debd33e0-47b9-401a-8eff-60827375008d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d33d92fd-19aa-46cb-a913-7772b5720ad7" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_debd33e0-47b9-401a-8eff-60827375008d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_66828947-8901-43c6-92f1-33ca647dc604" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_debd33e0-47b9-401a-8eff-60827375008d" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_66828947-8901-43c6-92f1-33ca647dc604" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_66828947-8901-43c6-92f1-33ca647dc604_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_66828947-8901-43c6-92f1-33ca647dc604" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_66828947-8901-43c6-92f1-33ca647dc604_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_aa910a4b-c766-4329-9227-d2cde54097b5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_66828947-8901-43c6-92f1-33ca647dc604" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_aa910a4b-c766-4329-9227-d2cde54097b5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_a4d7d099-4be0-4fd5-ae42-fb00a7603ce0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DefinedBenefitPostretirementHealthCoverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_aa910a4b-c766-4329-9227-d2cde54097b5" xlink:to="loc_us-gaap_DefinedBenefitPostretirementHealthCoverageMember_a4d7d099-4be0-4fd5-ae42-fb00a7603ce0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_debd33e0-47b9-401a-8eff-60827375008d" xlink:to="loc_srt_RangeAxis_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f" xlink:to="loc_srt_RangeMember_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_aaee8e85-7b34-4221-8409-21275c743190" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_0214a1c9-8e5d-4b1c-8486-2d155e0a7f6f" xlink:to="loc_srt_RangeMember_aaee8e85-7b34-4221-8409-21275c743190" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_5cad66d0-d0a9-4e13-b2ac-825942045492" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_aaee8e85-7b34-4221-8409-21275c743190" xlink:to="loc_srt_MinimumMember_5cad66d0-d0a9-4e13-b2ac-825942045492" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_a0b23609-80ee-460b-8a87-57938bf2e83c" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_aaee8e85-7b34-4221-8409-21275c743190" xlink:to="loc_srt_MaximumMember_a0b23609-80ee-460b-8a87-57938bf2e83c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockholdersEquity" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockholdersEquity" xlink:type="extended" id="i7545062b9ae545faabdff17cc29e1280_StockholdersEquity"/>
  <link:roleRef roleURI="http://www.xpo.com/role/StockholdersEquityTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockholdersEquityTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockholdersEquityTables" xlink:type="extended" id="ia2792db22346437bbef081414cca87ae_StockholdersEquityTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/StockholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockholdersEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockholdersEquityNarrativeDetails" xlink:type="extended" id="i1a37204f90c24620a9aa259de6cd7fda_StockholdersEquityNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:href="xpo-20201231.xsd#xpo_StockholdersEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PreferredStockIssuedDuringPeriodSharesNewShares_fe2a43fc-07e2-4937-ab64-4f1016fb0ec0" xlink:href="xpo-20201231.xsd#xpo_PreferredStockIssuedDuringPeriodSharesNewShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_xpo_PreferredStockIssuedDuringPeriodSharesNewShares_fe2a43fc-07e2-4937-ab64-4f1016fb0ec0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLiquidationPreference_5bbb7d3b-d96e-49df-b523-3a2e986784dc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TemporaryEquityLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_TemporaryEquityLiquidationPreference_5bbb7d3b-d96e-49df-b523-3a2e986784dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_55312aa0-0824-4b0a-87b4-bfd36f18278a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_55312aa0-0824-4b0a-87b4-bfd36f18278a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PreferredStockLiquidationPreferencePercentage_67eb0e25-70c2-489b-a24f-12a7010a121f" xlink:href="xpo-20201231.xsd#xpo_PreferredStockLiquidationPreferencePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_xpo_PreferredStockLiquidationPreferencePercentage_67eb0e25-70c2-489b-a24f-12a7010a121f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConversionOfStockSharesConverted1_5e714738-c380-4f04-9b41-d7e6991be94d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConversionOfStockSharesConverted1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_ConversionOfStockSharesConverted1_5e714738-c380-4f04-9b41-d7e6991be94d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PaymentInConnectionWithPreferredStockRedemption_496b4e25-2b53-4196-8af5-3f2f56aeda62" xlink:href="xpo-20201231.xsd#xpo_PaymentInConnectionWithPreferredStockRedemption"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_xpo_PaymentInConnectionWithPreferredStockRedemption_496b4e25-2b53-4196-8af5-3f2f56aeda62" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_575fc4e6-f84c-4e9b-8087-a2c157241c98" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_575fc4e6-f84c-4e9b-8087-a2c157241c98" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_da9bf455-2c15-4c39-887d-bebd15151606" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_da9bf455-2c15-4c39-887d-bebd15151606" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_ee5ee073-0b07-4205-a4e3-21a58e5db29e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_ee5ee073-0b07-4205-a4e3-21a58e5db29e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_0ab97988-2784-48e1-9878-5a225dd1d65d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_0ab97988-2784-48e1-9878-5a225dd1d65d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchaseProgramAuthorizedAmount1_721884d1-e4f5-4078-9a66-00740fff0480" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchaseProgramAuthorizedAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_us-gaap_StockRepurchaseProgramAuthorizedAmount1_721884d1-e4f5-4078-9a66-00740fff0480" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:href="xpo-20201231.xsd#xpo_StockholdersEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_xpo_StockholdersEquityLineItems_312ca923-813f-4457-852e-1096f683708c" xlink:to="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_d1f3deb8-135d-4345-a76a-b6a167eae912" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_d1f3deb8-135d-4345-a76a-b6a167eae912" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_d1f3deb8-135d-4345-a76a-b6a167eae912_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_d1f3deb8-135d-4345-a76a-b6a167eae912" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_d1f3deb8-135d-4345-a76a-b6a167eae912_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_1b566228-740e-4900-8693-bbb90b182e31" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_d1f3deb8-135d-4345-a76a-b6a167eae912" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_1b566228-740e-4900-8693-bbb90b182e31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_OfferedinConnectionWithForwardSaleAgreementsMember_60e14ab2-e93f-4d00-8e52-fd1aa5e8fc05" xlink:href="xpo-20201231.xsd#xpo_OfferedinConnectionWithForwardSaleAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_1b566228-740e-4900-8693-bbb90b182e31" xlink:to="loc_xpo_OfferedinConnectionWithForwardSaleAgreementsMember_60e14ab2-e93f-4d00-8e52-fd1aa5e8fc05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_OfferedDirectlyByTheCompanyMember_ec7d7daf-079e-49ae-8038-a02ae1ff8f37" xlink:href="xpo-20201231.xsd#xpo_OfferedDirectlyByTheCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_1b566228-740e-4900-8693-bbb90b182e31" xlink:to="loc_xpo_OfferedDirectlyByTheCompanyMember_ec7d7daf-079e-49ae-8038-a02ae1ff8f37" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:to="loc_us-gaap_StatementClassOfStockAxis_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3" xlink:to="loc_us-gaap_ClassOfStockDomain_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_660cef7b-62c6-4b64-81d2-427e6bc8445b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_9c3a16f2-2128-4d65-86bc-d4aa033fa3c3" xlink:to="loc_us-gaap_ClassOfStockDomain_660cef7b-62c6-4b64-81d2-427e6bc8445b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredStockMember_7cb06b4f-8766-4c27-a206-e12367cb8daa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_660cef7b-62c6-4b64-81d2-427e6bc8445b" xlink:to="loc_us-gaap_ConvertiblePreferredStockMember_7cb06b4f-8766-4c27-a206-e12367cb8daa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_26d6115f-eaa5-45ad-bf36-7dac5cc55ff7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_660cef7b-62c6-4b64-81d2-427e6bc8445b" xlink:to="loc_us-gaap_CommonStockMember_26d6115f-eaa5-45ad-bf36-7dac5cc55ff7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramAxis_e92deefa-1fe0-4296-abb5-17b253a77042" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:to="loc_us-gaap_ShareRepurchaseProgramAxis_e92deefa-1fe0-4296-abb5-17b253a77042" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_e92deefa-1fe0-4296-abb5-17b253a77042_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_e92deefa-1fe0-4296-abb5-17b253a77042" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_e92deefa-1fe0-4296-abb5-17b253a77042_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_59328fda-0e1d-42fd-83bd-34ede36d57bb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_e92deefa-1fe0-4296-abb5-17b253a77042" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_59328fda-0e1d-42fd-83bd-34ede36d57bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2018ProgramMember_72818c1e-a49f-4f23-ae0b-3a07a7d86ca4" xlink:href="xpo-20201231.xsd#xpo_A2018ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_59328fda-0e1d-42fd-83bd-34ede36d57bb" xlink:to="loc_xpo_A2018ProgramMember_72818c1e-a49f-4f23-ae0b-3a07a7d86ca4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2019ProgramMember_f8997dfa-394f-414c-b0c5-d7e0ab97e35f" xlink:href="xpo-20201231.xsd#xpo_A2019ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_59328fda-0e1d-42fd-83bd-34ede36d57bb" xlink:to="loc_xpo_A2019ProgramMember_f8997dfa-394f-414c-b0c5-d7e0ab97e35f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_0a66a0db-5fc4-4c9a-9301-d65256f64456" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_0a66a0db-5fc4-4c9a-9301-d65256f64456" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_0a66a0db-5fc4-4c9a-9301-d65256f64456_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_0a66a0db-5fc4-4c9a-9301-d65256f64456" xlink:to="loc_us-gaap_EquityComponentDomain_0a66a0db-5fc4-4c9a-9301-d65256f64456_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_411a9d3e-a2c6-4c65-8d86-bad720c8cdc4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_0a66a0db-5fc4-4c9a-9301-d65256f64456" xlink:to="loc_us-gaap_EquityComponentDomain_411a9d3e-a2c6-4c65-8d86-bad720c8cdc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_a8ea94ec-780b-409b-b24c-0b4d6e2afd8c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_411a9d3e-a2c6-4c65-8d86-bad720c8cdc4" xlink:to="loc_us-gaap_CommonStockMember_a8ea94ec-780b-409b-b24c-0b4d6e2afd8c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_67235d75-3d4d-48f3-97c5-a0405efe20ff" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_411a9d3e-a2c6-4c65-8d86-bad720c8cdc4" xlink:to="loc_us-gaap_PreferredStockMember_67235d75-3d4d-48f3-97c5-a0405efe20ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_9f301313-546c-49d8-92c7-26866f9c8649" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantMember_432156a2-4a8e-46da-9dcf-b14d85940351" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_WarrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_411a9d3e-a2c6-4c65-8d86-bad720c8cdc4" xlink:to="loc_us-gaap_WarrantMember_432156a2-4a8e-46da-9dcf-b14d85940351" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_0a8bcd06-96d6-4044-ba6d-4409adb57f10" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_xpo_StockholdersEquityTable_fe2cdaa9-061b-41dd-acd3-2348291aa8cb" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_0a8bcd06-96d6-4044-ba6d-4409adb57f10" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_0a8bcd06-96d6-4044-ba6d-4409adb57f10_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_0a8bcd06-96d6-4044-ba6d-4409adb57f10" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_0a8bcd06-96d6-4044-ba6d-4409adb57f10_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_82ac3a90-ea48-4af2-9a4d-d9d8f7147bd6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_0a8bcd06-96d6-4044-ba6d-4409adb57f10" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_82ac3a90-ea48-4af2-9a4d-d9d8f7147bd6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_895138b0-b0ad-4241-b5f5-3108d79afb02" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_82ac3a90-ea48-4af2-9a4d-d9d8f7147bd6" xlink:to="loc_us-gaap_SubsequentEventMember_895138b0-b0ad-4241-b5f5-3108d79afb02" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockholdersEquityScheduleofSharesRepurchasedDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockholdersEquityScheduleofSharesRepurchasedDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockholdersEquityScheduleofSharesRepurchasedDetails" xlink:type="extended" id="i216e4ac3ad574e3d98d1e5ca7c5e0841_StockholdersEquityScheduleofSharesRepurchasedDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EquityClassOfTreasuryStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockSharesAcquired_8271509c-1194-4d45-ad7d-86ed0b3dcb5a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockSharesAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:to="loc_us-gaap_TreasuryStockSharesAcquired_8271509c-1194-4d45-ad7d-86ed0b3dcb5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_6f4a222e-56d3-4d87-8147-f01c4970490f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockValueAcquiredCostMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:to="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_6f4a222e-56d3-4d87-8147-f01c4970490f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_857b21f9-5375-46c4-88b1-470a3ab5ddee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TreasuryStockAcquiredAverageCostPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:to="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_857b21f9-5375-46c4-88b1-470a3ab5ddee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1_33b1d583-28c4-4cf9-ab57-dc5808aea927" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:to="loc_us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1_33b1d583-28c4-4cf9-ab57-dc5808aea927" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfTreasuryStockTable_4caea02c-725e-48f8-936a-2cb15d02b135" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ClassOfTreasuryStockTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EquityClassOfTreasuryStockLineItems_e0a2911b-55e5-4c69-bbc1-ea386219afe4" xlink:to="loc_us-gaap_ClassOfTreasuryStockTable_4caea02c-725e-48f8-936a-2cb15d02b135" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramAxis_785239bc-3357-46df-97ad-d373a1be69ce" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ClassOfTreasuryStockTable_4caea02c-725e-48f8-936a-2cb15d02b135" xlink:to="loc_us-gaap_ShareRepurchaseProgramAxis_785239bc-3357-46df-97ad-d373a1be69ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_785239bc-3357-46df-97ad-d373a1be69ce_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_785239bc-3357-46df-97ad-d373a1be69ce" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_785239bc-3357-46df-97ad-d373a1be69ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_54f6cd15-c54e-43fc-8cc5-0a89039d367e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_785239bc-3357-46df-97ad-d373a1be69ce" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_54f6cd15-c54e-43fc-8cc5-0a89039d367e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2020ProgramMember_ee79a392-9a82-4d3d-89d8-a1eab9abf120" xlink:href="xpo-20201231.xsd#xpo_A2020ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_54f6cd15-c54e-43fc-8cc5-0a89039d367e" xlink:to="loc_xpo_A2020ProgramMember_ee79a392-9a82-4d3d-89d8-a1eab9abf120" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2019ProgramMember_2e3f92e5-e4cc-43de-8a86-43ae8cf13553" xlink:href="xpo-20201231.xsd#xpo_A2019ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_54f6cd15-c54e-43fc-8cc5-0a89039d367e" xlink:to="loc_xpo_A2019ProgramMember_2e3f92e5-e4cc-43de-8a86-43ae8cf13553" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2018ProgramMember_24f409b8-b734-4cc0-a3c4-4296624a2ecc" xlink:href="xpo-20201231.xsd#xpo_A2018ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_54f6cd15-c54e-43fc-8cc5-0a89039d367e" xlink:to="loc_xpo_A2018ProgramMember_24f409b8-b734-4cc0-a3c4-4296624a2ecc" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensation" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensation"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensation" xlink:type="extended" id="i8d1f9aa5446747d3930d6745977ce960_StockBasedCompensation"/>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensationTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensationTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensationTables" xlink:type="extended" id="i5519546f2d7a4d419dbdb23c6f353927_StockBasedCompensationTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensationNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensationNarrativeDetails" xlink:type="extended" id="i910b35fb09c143bfbb0f24c61b2cffe8_StockBasedCompensationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_53a3d6b7-12c8-4c50-96c6-c549af2dd92f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_53a3d6b7-12c8-4c50-96c6-c549af2dd92f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_2952bc6f-df5f-4463-a87b-8c1e4a2347e5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_2952bc6f-df5f-4463-a87b-8c1e4a2347e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeStockPurchasePlanMaximumEmployeeContributionPercent_a29c99ef-7fc6-4575-8cf1-a55b00fd4515" xlink:href="xpo-20201231.xsd#xpo_EmployeeStockPurchasePlanMaximumEmployeeContributionPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_EmployeeStockPurchasePlanMaximumEmployeeContributionPercent_a29c99ef-7fc6-4575-8cf1-a55b00fd4515" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeStockPurchasePlanDiscountFromMarketRate_fdbe44a7-0294-4d05-afce-bd13ba1cf2d2" xlink:href="xpo-20201231.xsd#xpo_EmployeeStockPurchasePlanDiscountFromMarketRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_EmployeeStockPurchasePlanDiscountFromMarketRate_fdbe44a7-0294-4d05-afce-bd13ba1cf2d2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeStockPurchasePlanOfferingPeriod_3b0b8def-fb9d-40b5-87c8-93ead4c4a21a" xlink:href="xpo-20201231.xsd#xpo_EmployeeStockPurchasePlanOfferingPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_EmployeeStockPurchasePlanOfferingPeriod_3b0b8def-fb9d-40b5-87c8-93ead4c4a21a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeStockPurchasePlanNumberOfSharesAuthorizedForIssuance_2d719242-606f-4777-a0f1-f25bc028d5dc" xlink:href="xpo-20201231.xsd#xpo_EmployeeStockPurchasePlanNumberOfSharesAuthorizedForIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_EmployeeStockPurchasePlanNumberOfSharesAuthorizedForIssuance_2d719242-606f-4777-a0f1-f25bc028d5dc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeStockPurchasePlanNumberOfSharesAvailableToBeGranted_ca252824-dbbf-4491-a1e4-752ac43d908c" xlink:href="xpo-20201231.xsd#xpo_EmployeeStockPurchasePlanNumberOfSharesAvailableToBeGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_EmployeeStockPurchasePlanNumberOfSharesAvailableToBeGranted_ca252824-dbbf-4491-a1e4-752ac43d908c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_b75ee87b-16ca-48f8-960e-daf600aef69c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_b75ee87b-16ca-48f8-960e-daf600aef69c" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_1eb44d88-e1ca-4e9f-8bf8-15b5dcf04f91" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_1eb44d88-e1ca-4e9f-8bf8-15b5dcf04f91" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_8390fc79-ec86-4ef6-821a-49c0d80a9ce6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_8390fc79-ec86-4ef6-821a-49c0d80a9ce6" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_72fbcefc-ff82-495b-827a-b8bb1258ac7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_72fbcefc-ff82-495b-827a-b8bb1258ac7a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue_070bc428-c0ed-4cf5-a9a4-55a0af1bf5ad" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodTotalIntrinsicValue_070bc428-c0ed-4cf5-a9a4-55a0af1bf5ad" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_79dcb622-8116-4113-bfd6-b5bb13c4235e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_79dcb622-8116-4113-bfd6-b5bb13c4235e" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_df4a1bab-61ff-4e94-867b-b4fbc859b4df" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_df4a1bab-61ff-4e94-867b-b4fbc859b4df" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_477fbdaf-bbcf-4eac-ba5a-31a8616d8eb6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_477fbdaf-bbcf-4eac-ba5a-31a8616d8eb6" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_5036a883-30ab-482b-829b-a24640a04b71" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_5036a883-30ab-482b-829b-a24640a04b71" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_2d808817-9b5f-419b-9d51-e12229b6ff70" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_2d808817-9b5f-419b-9d51-e12229b6ff70" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_a99d39fe-87b3-4148-a3f1-9be5fa45bdb6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_a99d39fe-87b3-4148-a3f1-9be5fa45bdb6" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_07b4500a-718c-41d6-8424-053e431e2f6b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_07b4500a-718c-41d6-8424-053e431e2f6b" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_bfeb24a7-5e04-4af6-9398-b58c0fc74ad8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_bfeb24a7-5e04-4af6-9398-b58c0fc74ad8" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_e76ebc50-4941-4b26-9c24-13dc0e1dddd5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_e76ebc50-4941-4b26-9c24-13dc0e1dddd5" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_b4a3272e-986d-4cff-948b-07325745d87f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_b4a3272e-986d-4cff-948b-07325745d87f" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCompensationCashbasedArrangementsLiabilityCurrentAndNoncurrent_6bc49937-28b7-4aa2-bcd7-1b52d8fbcef0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredCompensationCashbasedArrangementsLiabilityCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_DeferredCompensationCashbasedArrangementsLiabilityCurrentAndNoncurrent_6bc49937-28b7-4aa2-bcd7-1b52d8fbcef0" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageFairValue_50e97df6-4e93-4b4b-9604-770bc81a68c8" xlink:href="xpo-20201231.xsd#xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageFairValue_50e97df6-4e93-4b4b-9604-770bc81a68c8" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_b8be84b5-01ed-4ae1-a2e0-7e60665675fd" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_a03cbd55-4ae0-4b0e-bc10-16de6444ff41" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:to="loc_us-gaap_PlanNameAxis_a03cbd55-4ae0-4b0e-bc10-16de6444ff41" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_a03cbd55-4ae0-4b0e-bc10-16de6444ff41_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_a03cbd55-4ae0-4b0e-bc10-16de6444ff41" xlink:to="loc_us-gaap_PlanNameDomain_a03cbd55-4ae0-4b0e-bc10-16de6444ff41_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_3a7bb575-1646-492c-a112-541437bb3cfa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_a03cbd55-4ae0-4b0e-bc10-16de6444ff41" xlink:to="loc_us-gaap_PlanNameDomain_3a7bb575-1646-492c-a112-541437bb3cfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_A2016OmnibusIncentiveCompensationPlanMember_a74a8af9-06d8-4976-8ddf-386d098b6c6e" xlink:href="xpo-20201231.xsd#xpo_A2016OmnibusIncentiveCompensationPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_3a7bb575-1646-492c-a112-541437bb3cfa" xlink:to="loc_xpo_A2016OmnibusIncentiveCompensationPlanMember_a74a8af9-06d8-4976-8ddf-386d098b6c6e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_13840548-b54d-4fe3-9b12-b58d00d3c532" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:to="loc_us-gaap_AwardTypeAxis_13840548-b54d-4fe3-9b12-b58d00d3c532" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_13840548-b54d-4fe3-9b12-b58d00d3c532_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_13840548-b54d-4fe3-9b12-b58d00d3c532" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_13840548-b54d-4fe3-9b12-b58d00d3c532_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_13840548-b54d-4fe3-9b12-b58d00d3c532" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_e8021cb2-e256-4dc0-8f27-d4b0dd966c00" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_us-gaap_EmployeeStockMember_e8021cb2-e256-4dc0-8f27-d4b0dd966c00" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_7dcf45a4-328b-40ad-b146-9a5cd5cd5202" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_us-gaap_EmployeeStockOptionMember_7dcf45a4-328b-40ad-b146-9a5cd5cd5202" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockMember_279b3987-b61b-487d-bf44-cfad0ce2aec1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_us-gaap_RestrictedStockMember_279b3987-b61b-487d-bf44-cfad0ce2aec1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_1768064a-e2e3-481b-b295-831faece8066" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_1768064a-e2e3-481b-b295-831faece8066" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_a11e2bdc-579f-416b-ab1e-390db8e9c4d0" xlink:href="xpo-20201231.xsd#xpo_PerformanceBasedRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_a11e2bdc-579f-416b-ab1e-390db8e9c4d0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PerformancebasedRestrictedStockUnitsSubjectToServiceMarketandPerformanceBasedConditionsMember_050e6245-160b-4b75-9ba5-a5248a6dc47b" xlink:href="xpo-20201231.xsd#xpo_PerformancebasedRestrictedStockUnitsSubjectToServiceMarketandPerformanceBasedConditionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_xpo_PerformancebasedRestrictedStockUnitsSubjectToServiceMarketandPerformanceBasedConditionsMember_050e6245-160b-4b75-9ba5-a5248a6dc47b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_RestrictedStockUnitsSubjectToServiceandPerformanceConditionsMember_876eb736-8fe4-47ac-ba8b-a5201dce3239" xlink:href="xpo-20201231.xsd#xpo_RestrictedStockUnitsSubjectToServiceandPerformanceConditionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_xpo_RestrictedStockUnitsSubjectToServiceandPerformanceConditionsMember_876eb736-8fe4-47ac-ba8b-a5201dce3239" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_RestrictedStockUnitsSubjectToServiceAndMarketConditionsMember_9ac0b78e-db1c-4b14-8fe5-c5b92245c73c" xlink:href="xpo-20201231.xsd#xpo_RestrictedStockUnitsSubjectToServiceAndMarketConditionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_xpo_RestrictedStockUnitsSubjectToServiceAndMarketConditionsMember_9ac0b78e-db1c-4b14-8fe5-c5b92245c73c" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_RestrictedStockUnitsandPerformancebasedRestrictedStockUnitsMember_a538f31d-82d2-475f-ab39-acd278f25a15" xlink:href="xpo-20201231.xsd#xpo_RestrictedStockUnitsandPerformancebasedRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_xpo_RestrictedStockUnitsandPerformancebasedRestrictedStockUnitsMember_a538f31d-82d2-475f-ab39-acd278f25a15" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_d77674ed-899b-4de9-99f2-0797878bd79e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a93be011-f4bb-45d5-8dcb-65cb02561b6e" xlink:to="loc_us-gaap_PerformanceSharesMember_d77674ed-899b-4de9-99f2-0797878bd79e" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_8a40194c-25ec-4e8c-b153-441042edb006" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:to="loc_srt_TitleOfIndividualAxis_8a40194c-25ec-4e8c-b153-441042edb006" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_8a40194c-25ec-4e8c-b153-441042edb006_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_8a40194c-25ec-4e8c-b153-441042edb006" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_8a40194c-25ec-4e8c-b153-441042edb006_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_379614fd-c468-4266-8cab-26873129c7ec" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_8a40194c-25ec-4e8c-b153-441042edb006" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_379614fd-c468-4266-8cab-26873129c7ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_EmployeeandOfficerMember_e958a801-1da1-46f7-8629-ba7ab0d957a5" xlink:href="xpo-20201231.xsd#xpo_EmployeeandOfficerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_379614fd-c468-4266-8cab-26873129c7ec" xlink:to="loc_xpo_EmployeeandOfficerMember_e958a801-1da1-46f7-8629-ba7ab0d957a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_DirectorMember_8b5ba7ee-0f19-4895-a147-a658ec725978" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_DirectorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_379614fd-c468-4266-8cab-26873129c7ec" xlink:to="loc_srt_DirectorMember_8b5ba7ee-0f19-4895-a147-a658ec725978" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_0c5c6e2e-6471-45d4-a791-24afec6ea005" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:to="loc_srt_RangeAxis_0c5c6e2e-6471-45d4-a791-24afec6ea005" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0c5c6e2e-6471-45d4-a791-24afec6ea005_default" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_0c5c6e2e-6471-45d4-a791-24afec6ea005" xlink:to="loc_srt_RangeMember_0c5c6e2e-6471-45d4-a791-24afec6ea005_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_bd4e3768-fe0e-4996-98d4-8160912d0564" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_0c5c6e2e-6471-45d4-a791-24afec6ea005" xlink:to="loc_srt_RangeMember_bd4e3768-fe0e-4996-98d4-8160912d0564" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_3b3607b8-7b5e-4531-a32d-3c92720ca9fa" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_bd4e3768-fe0e-4996-98d4-8160912d0564" xlink:to="loc_srt_MinimumMember_3b3607b8-7b5e-4531-a32d-3c92720ca9fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_7d195c1f-21b5-44b7-b7f3-8ff7303333cd" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_bd4e3768-fe0e-4996-98d4-8160912d0564" xlink:to="loc_srt_MaximumMember_7d195c1f-21b5-44b7-b7f3-8ff7303333cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingAxis_90225ac9-730e-4023-87be-453ccdf2d823" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8085c89c-ba11-4bcf-9733-2e3dbe1fcd58" xlink:to="loc_us-gaap_VestingAxis_90225ac9-730e-4023-87be-453ccdf2d823" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_90225ac9-730e-4023-87be-453ccdf2d823_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VestingAxis_90225ac9-730e-4023-87be-453ccdf2d823" xlink:to="loc_us-gaap_VestingDomain_90225ac9-730e-4023-87be-453ccdf2d823_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VestingDomain_d165285c-8556-4543-aea2-00b931fe672d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_VestingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VestingAxis_90225ac9-730e-4023-87be-453ccdf2d823" xlink:to="loc_us-gaap_VestingDomain_d165285c-8556-4543-aea2-00b931fe672d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_e17c4a6a-9787-4608-8ab8-c0fd28d15ca7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationAwardTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VestingDomain_d165285c-8556-4543-aea2-00b931fe672d" xlink:to="loc_us-gaap_ShareBasedCompensationAwardTrancheOneMember_e17c4a6a-9787-4608-8ab8-c0fd28d15ca7" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensationScheduleofStockbasedCompensationExpenseDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensationScheduleofStockbasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensationScheduleofStockbasedCompensationExpenseDetails" xlink:type="extended" id="ib84c644142384ab58de878591e786d4b_StockBasedCompensationScheduleofStockbasedCompensationExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_7bff76e8-fc37-4021-ab49-da618050efb3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_f4cd90e1-b820-4e60-a520-fe1151a75f85" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_7bff76e8-fc37-4021-ab49-da618050efb3" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_f4cd90e1-b820-4e60-a520-fe1151a75f85" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_8a4df22c-3a55-4a04-bf80-761ef435144b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_7bff76e8-fc37-4021-ab49-da618050efb3" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_8a4df22c-3a55-4a04-bf80-761ef435144b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_487a50a7-a9db-4c0d-8537-f8328ae06ec9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_7bff76e8-fc37-4021-ab49-da618050efb3" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_487a50a7-a9db-4c0d-8537-f8328ae06ec9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_712d9b06-f312-45a8-96ed-5cc93d4381fe" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_487a50a7-a9db-4c0d-8537-f8328ae06ec9" xlink:to="loc_us-gaap_AwardTypeAxis_712d9b06-f312-45a8-96ed-5cc93d4381fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_712d9b06-f312-45a8-96ed-5cc93d4381fe_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_712d9b06-f312-45a8-96ed-5cc93d4381fe" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_712d9b06-f312-45a8-96ed-5cc93d4381fe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3929f71-fb65-40f0-a798-4e01a26c25b9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_712d9b06-f312-45a8-96ed-5cc93d4381fe" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3929f71-fb65-40f0-a798-4e01a26c25b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_3c344c54-cc40-436c-9373-e931fc3d927a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3929f71-fb65-40f0-a798-4e01a26c25b9" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_3c344c54-cc40-436c-9373-e931fc3d927a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_f780c75a-ee95-429b-b1dc-c48986cf0fdf" xlink:href="xpo-20201231.xsd#xpo_PerformanceBasedRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3929f71-fb65-40f0-a798-4e01a26c25b9" xlink:to="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_f780c75a-ee95-429b-b1dc-c48986cf0fdf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_407be13f-ff92-4762-8944-5ba86c581e34" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3929f71-fb65-40f0-a798-4e01a26c25b9" xlink:to="loc_us-gaap_PerformanceSharesMember_407be13f-ff92-4762-8944-5ba86c581e34" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensationEquityAwardsOutstandingandExercisableDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensationEquityAwardsOutstandingandExercisableDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensationEquityAwardsOutstandingandExercisableDetails" xlink:type="extended" id="ibc6e74924dee4202b332958bc8c8500c_StockBasedCompensationEquityAwardsOutstandingandExercisableDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_d396b8ef-7012-4fc7-a47b-5ca4b0a05cd7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_d396b8ef-7012-4fc7-a47b-5ca4b0a05cd7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1ccd8572-045f-4c64-bbab-670c29989515" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1ccd8572-045f-4c64-bbab-670c29989515" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2219ea5b-bfca-47a1-a317-6497160ba398" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2219ea5b-bfca-47a1-a317-6497160ba398" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_d12e394c-4e0d-4224-b424-a8c8b0815224" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_48efc3d7-0d02-49f8-983d-0722c79310f3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_d12e394c-4e0d-4224-b424-a8c8b0815224" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_33bd488b-ae0f-412d-8f73-2b030cc2feab" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_aec33470-5eed-4477-bfd8-012af77b9538" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_aec33470-5eed-4477-bfd8-012af77b9538" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_daf34515-3110-489d-80ae-37e646e5fbe4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_daf34515-3110-489d-80ae-37e646e5fbe4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_58dcfc43-2bc0-4c13-b52f-63fded495cf7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_58dcfc43-2bc0-4c13-b52f-63fded495cf7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_63a6f7e5-b1fd-4d78-96e1-52b9fdc92c49" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_63a6f7e5-b1fd-4d78-96e1-52b9fdc92c49" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_5e53e328-d277-4e1a-9b12-e94a09fdf070" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_05b70bbf-7aca-4130-a008-47dbfe4f5d71" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_5e53e328-d277-4e1a-9b12-e94a09fdf070" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_06cd8507-8435-4b7c-a875-fa3b1580e8e3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_7629da1c-88ff-41eb-b514-02d0611b00d8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_7629da1c-88ff-41eb-b514-02d0611b00d8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_77e79b8a-add1-4635-ad4b-481603a21f92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_77e79b8a-add1-4635-ad4b-481603a21f92" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_f941eea6-b2f0-445b-97b8-0717f05f1f65" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_77e79b8a-add1-4635-ad4b-481603a21f92" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_f941eea6-b2f0-445b-97b8-0717f05f1f65" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1_bb5ddd1a-c705-4be4-afb8-40c36ece3f98" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_77e79b8a-add1-4635-ad4b-481603a21f92" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsExercisableWeightedAverageRemainingContractualTerm1_bb5ddd1a-c705-4be4-afb8-40c36ece3f98" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_482fd8eb-adb0-4b77-b270-54952f40d103" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_482fd8eb-adb0-4b77-b270-54952f40d103" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted_30386fa7-509a-43de-a743-c6f636eb12af" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted_30386fa7-509a-43de-a743-c6f636eb12af" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsVested_d3d776db-5813-4822-bb49-66d2d4acf8ae" xlink:href="xpo-20201231.xsd#xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsVested"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:to="loc_xpo_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsVested_d3d776db-5813-4822-bb49-66d2d4acf8ae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeitures_12b5dcf0-8d1f-4f16-a11c-29492688249c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingRollForward_568ac1a5-0570-483d-9432-eabaf90e88b1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeitures_12b5dcf0-8d1f-4f16-a11c-29492688249c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_cfa5f97f-80a3-4906-8825-eb0675ef6eb8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_efd2e818-2768-45a5-966b-1925aac93eb2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_efd2e818-2768-45a5-966b-1925aac93eb2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_d3a5e1d8-3dfb-48a4-b0cc-61c34cb71a0b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_d3a5e1d8-3dfb-48a4-b0cc-61c34cb71a0b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_60b39eca-3af3-4c95-8d7f-1de13f6f9259" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_60b39eca-3af3-4c95-8d7f-1de13f6f9259" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_a25d8270-0626-49e8-a3e8-944a5121d0db" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_aa7b9300-4f20-4dd7-b193-b89bb34d57c1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_a25d8270-0626-49e8-a3e8-944a5121d0db" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_bf8ddf82-b110-491c-a40b-c6a122bfd7d0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_614692b3-9a9c-4740-af81-274dcf62ab96" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a852edac-9de6-4d94-8e47-e23c059252f1" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_614692b3-9a9c-4740-af81-274dcf62ab96" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_df0d6247-b8ea-47ea-aace-51c359c09f9b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_614692b3-9a9c-4740-af81-274dcf62ab96" xlink:to="loc_us-gaap_AwardTypeAxis_df0d6247-b8ea-47ea-aace-51c359c09f9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_df0d6247-b8ea-47ea-aace-51c359c09f9b_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_df0d6247-b8ea-47ea-aace-51c359c09f9b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_df0d6247-b8ea-47ea-aace-51c359c09f9b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_df0d6247-b8ea-47ea-aace-51c359c09f9b" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_1f791c11-340a-40d5-99af-8fe747b02dd6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:to="loc_us-gaap_EmployeeStockOptionMember_1f791c11-340a-40d5-99af-8fe747b02dd6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_31a7d104-a3ff-4dab-95e0-74a6153998f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_31a7d104-a3ff-4dab-95e0-74a6153998f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_6500811b-17ba-4d1e-a416-1a50d52fc313" xlink:href="xpo-20201231.xsd#xpo_PerformanceBasedRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:to="loc_xpo_PerformanceBasedRestrictedStockUnitsMember_6500811b-17ba-4d1e-a416-1a50d52fc313" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_d741dd66-65cc-45f1-a497-c29ba9314d82" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_91fe6ce1-e97e-4c4f-bfaf-0bb50de86682" xlink:to="loc_us-gaap_PerformanceSharesMember_d741dd66-65cc-45f1-a497-c29ba9314d82" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/StockBasedCompensationDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#StockBasedCompensationDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/StockBasedCompensationDetails" xlink:type="extended" id="ia157b8bc1cbd41f3a7d6f1910afa0af1_StockBasedCompensationDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxes" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxes"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxes" xlink:type="extended" id="ia98a5b6c064b4328966fdf49f06c178c_IncomeTaxes"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesTables" xlink:type="extended" id="ifdcd9f7b60ae45bcaaaf7f2cf254eef5_IncomeTaxesTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofIncomebeforeIncomeTaxRelatedtoDomesticandForeignDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofIncomebeforeIncomeTaxRelatedtoDomesticandForeignDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofIncomebeforeIncomeTaxRelatedtoDomesticandForeignDetails" xlink:type="extended" id="i2664c9f889ba4ad8965dd680560e8ee1_IncomeTaxesScheduleofIncomebeforeIncomeTaxRelatedtoDomesticandForeignDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofComponentsofIncomeTaxExpenseBenefitDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofComponentsofIncomeTaxExpenseBenefitDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofComponentsofIncomeTaxExpenseBenefitDetails" xlink:type="extended" id="ic486216d8dae4b09bb398430e3e32adf_IncomeTaxesScheduleofComponentsofIncomeTaxExpenseBenefitDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofEffectiveIncomeTaxRateReconciliationDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofEffectiveIncomeTaxRateReconciliationDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofEffectiveIncomeTaxRateReconciliationDetails" xlink:type="extended" id="i3de0236654d644a28a3283642a77b9fc_IncomeTaxesScheduleofEffectiveIncomeTaxRateReconciliationDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofDeferredTaxAssetsandLiabilitiesDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofDeferredTaxAssetsandLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofDeferredTaxAssetsandLiabilitiesDetails" xlink:type="extended" id="id2f072f72231442b8ab04627d09f698f_IncomeTaxesScheduleofDeferredTaxAssetsandLiabilitiesDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofOperatingLossandTaxCreditCarryforwardsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofOperatingLossandTaxCreditCarryforwardsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofOperatingLossandTaxCreditCarryforwardsDetails" xlink:type="extended" id="i4e6e5556e754461298a567d9e3dbac89_IncomeTaxesScheduleofOperatingLossandTaxCreditCarryforwardsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_d2d2406c-bae6-4b1c-89a0-75ea67b4020a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_f8fad59a-3678-4de9-9c3d-73af20e9cecd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_d2d2406c-bae6-4b1c-89a0-75ea67b4020a" xlink:to="loc_us-gaap_OperatingLossCarryforwards_f8fad59a-3678-4de9-9c3d-73af20e9cecd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardAmount_02133f09-8e0e-4354-ab57-660935089bc6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_TaxCreditCarryforwardAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_d2d2406c-bae6-4b1c-89a0-75ea67b4020a" xlink:to="loc_us-gaap_TaxCreditCarryforwardAmount_02133f09-8e0e-4354-ab57-660935089bc6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_e44c4ff6-aa22-4024-8980-80a192d0a15b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_d2d2406c-bae6-4b1c-89a0-75ea67b4020a" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_e44c4ff6-aa22-4024-8980-80a192d0a15b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_8d8c74a7-e0f3-46cc-a961-30639e35055a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_e44c4ff6-aa22-4024-8980-80a192d0a15b" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_8d8c74a7-e0f3-46cc-a961-30639e35055a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_8d8c74a7-e0f3-46cc-a961-30639e35055a_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_8d8c74a7-e0f3-46cc-a961-30639e35055a" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_8d8c74a7-e0f3-46cc-a961-30639e35055a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_8d8c74a7-e0f3-46cc-a961-30639e35055a" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalRevenueServiceIRSMember_b74d393d-c590-4968-b24e-171d67065afd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InternalRevenueServiceIRSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:to="loc_us-gaap_InternalRevenueServiceIRSMember_b74d393d-c590-4968-b24e-171d67065afd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_170a0f0a-3b5d-4127-aa03-2d00c63c6775" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_170a0f0a-3b5d-4127-aa03-2d00c63c6775" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_30f063d1-ff6f-4aca-a6ba-4627fb5bc095" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DomesticCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:to="loc_us-gaap_DomesticCountryMember_30f063d1-ff6f-4aca-a6ba-4627fb5bc095" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCountryMember_be729625-9265-47bd-8f42-46ea974aa46e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ForeignCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_de26a6da-23cf-4d71-90e2-3042b0223d19" xlink:to="loc_us-gaap_ForeignCountryMember_be729625-9265-47bd-8f42-46ea974aa46e" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesChangeinDeferredTaxAssetValuationAllowanceDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesChangeinDeferredTaxAssetValuationAllowanceDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesChangeinDeferredTaxAssetValuationAllowanceDetails" xlink:type="extended" id="i8e1787033f5949b98944acfae0b65039_IncomeTaxesChangeinDeferredTaxAssetValuationAllowanceDetails">
    <link:loc xlink:type="locator" xlink:label="loc_srt_ValuationAndQualifyingAccountsDisclosureLineItems_957ec435-a78c-4269-8137-8e698a3207f7" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ValuationAndQualifyingAccountsDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MovementInValuationAllowancesAndReservesRollForward_d7ae4673-22e7-436b-b34e-8a1d27ee7680" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_MovementInValuationAllowancesAndReservesRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ValuationAndQualifyingAccountsDisclosureLineItems_957ec435-a78c-4269-8137-8e698a3207f7" xlink:to="loc_us-gaap_MovementInValuationAllowancesAndReservesRollForward_d7ae4673-22e7-436b-b34e-8a1d27ee7680" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesBalance_aeee30e4-23ca-470d-8ca5-54e4de5437d7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesBalance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MovementInValuationAllowancesAndReservesRollForward_d7ae4673-22e7-436b-b34e-8a1d27ee7680" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesBalance_aeee30e4-23ca-470d-8ca5-54e4de5437d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesChargedToCostAndExpense_5f9ab964-2b76-48a0-b8dd-2bc3e57598ed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesChargedToCostAndExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MovementInValuationAllowancesAndReservesRollForward_d7ae4673-22e7-436b-b34e-8a1d27ee7680" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesChargedToCostAndExpense_5f9ab964-2b76-48a0-b8dd-2bc3e57598ed" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesDeductions_860c51c7-3ace-4ae9-86e9-e6a3a6f6c1aa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesDeductions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MovementInValuationAllowancesAndReservesRollForward_d7ae4673-22e7-436b-b34e-8a1d27ee7680" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesDeductions_860c51c7-3ace-4ae9-86e9-e6a3a6f6c1aa" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesBalance_910ae28a-afa3-442d-bc63-8e55b0165866" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesBalance"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ValuationAndQualifyingAccountsDisclosureTable_afc00f0f-d609-475c-b2d8-a23bc47c93d6" xlink:href="http://xbrl.fasb.org/srt/2020/elts/srt-2020-01-31.xsd#srt_ValuationAndQualifyingAccountsDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_srt_ValuationAndQualifyingAccountsDisclosureLineItems_957ec435-a78c-4269-8137-8e698a3207f7" xlink:to="loc_srt_ValuationAndQualifyingAccountsDisclosureTable_afc00f0f-d609-475c-b2d8-a23bc47c93d6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesTypeAxis_05e58e64-e753-48bd-b120-ea19968c4fa4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_srt_ValuationAndQualifyingAccountsDisclosureTable_afc00f0f-d609-475c-b2d8-a23bc47c93d6" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesTypeAxis_05e58e64-e753-48bd-b120-ea19968c4fa4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesDomain_05e58e64-e753-48bd-b120-ea19968c4fa4_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationAllowancesAndReservesTypeAxis_05e58e64-e753-48bd-b120-ea19968c4fa4" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesDomain_05e58e64-e753-48bd-b120-ea19968c4fa4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowancesAndReservesDomain_29645631-68b3-4b3d-9aba-7fa59af199f8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowancesAndReservesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationAllowancesAndReservesTypeAxis_05e58e64-e753-48bd-b120-ea19968c4fa4" xlink:to="loc_us-gaap_ValuationAllowancesAndReservesDomain_29645631-68b3-4b3d-9aba-7fa59af199f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationAllowanceOfDeferredTaxAssetsMember_48a8db2b-a9b0-420b-9984-5b8df7ebdc24" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ValuationAllowanceOfDeferredTaxAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationAllowancesAndReservesDomain_29645631-68b3-4b3d-9aba-7fa59af199f8" xlink:to="loc_us-gaap_ValuationAllowanceOfDeferredTaxAssetsMember_48a8db2b-a9b0-420b-9984-5b8df7ebdc24" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesScheduleofUnrecognizedTaxBenefitsRollForwardDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesScheduleofUnrecognizedTaxBenefitsRollForwardDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesScheduleofUnrecognizedTaxBenefitsRollForwardDetails" xlink:type="extended" id="ie2f7399ba5c3401da7dab9c9375727db_IncomeTaxesScheduleofUnrecognizedTaxBenefitsRollForwardDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended" id="icc06dc0f8c1f4ca4a730f18578b1a603_IncomeTaxesNarrativeDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/EarningsperShare" xlink:type="simple" xlink:href="xpo-20201231.xsd#EarningsperShare"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EarningsperShare" xlink:type="extended" id="ia8996e6720bd489085e8f3b7182450ad_EarningsperShare"/>
  <link:roleRef roleURI="http://www.xpo.com/role/EarningsperShareTables" xlink:type="simple" xlink:href="xpo-20201231.xsd#EarningsperShareTables"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EarningsperShareTables" xlink:type="extended" id="id72812a6f4c54bf6a5ea19e23a6bb889_EarningsperShareTables"/>
  <link:roleRef roleURI="http://www.xpo.com/role/EarningsperShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#EarningsperShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/EarningsperShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="extended" id="i91b39588d3414a09bb04c5a90061a28e_EarningsperShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails"/>
  <link:roleRef roleURI="http://www.xpo.com/role/CommitmentsandContingencies" xlink:type="simple" xlink:href="xpo-20201231.xsd#CommitmentsandContingencies"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/CommitmentsandContingencies" xlink:type="extended" id="iee8ddc0ea96445618e392f7410d3eec1_CommitmentsandContingencies"/>
  <link:roleRef roleURI="http://www.xpo.com/role/SubsequentEvents" xlink:type="simple" xlink:href="xpo-20201231.xsd#SubsequentEvents"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SubsequentEvents" xlink:type="extended" id="i3167e97b08cb4e8989fde6d324945ecd_SubsequentEvents"/>
  <link:roleRef roleURI="http://www.xpo.com/role/SubsequentEventsDetails" xlink:type="simple" xlink:href="xpo-20201231.xsd#SubsequentEventsDetails"/>
  <link:definitionLink xlink:role="http://www.xpo.com/role/SubsequentEventsDetails" xlink:type="extended" id="iba81dbf42ff64289918c25a5ac0cd6ae_SubsequentEventsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_86f08c65-e9ef-4f61-b8aa-b2f6281b5ce2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_4e1cd7d5-56d0-49f6-8f6e-a57353fe3ece" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_86f08c65-e9ef-4f61-b8aa-b2f6281b5ce2" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_4e1cd7d5-56d0-49f6-8f6e-a57353fe3ece" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_755a01b4-c93d-445a-aa74-273a2cd9032a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentRedemptionPricePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_86f08c65-e9ef-4f61-b8aa-b2f6281b5ce2" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPricePercentage_755a01b4-c93d-445a-aa74-273a2cd9032a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_00658b1f-fee9-429c-9526-0665bca3c489" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_86f08c65-e9ef-4f61-b8aa-b2f6281b5ce2" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_00658b1f-fee9-429c-9526-0665bca3c489" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_ca897b1f-1cfa-4a2d-a7a4-5c68f94f6020" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_86f08c65-e9ef-4f61-b8aa-b2f6281b5ce2" xlink:to="loc_us-gaap_SubsequentEventTable_ca897b1f-1cfa-4a2d-a7a4-5c68f94f6020" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_0c8516eb-d314-4dd3-aaeb-074463a60d50" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_ca897b1f-1cfa-4a2d-a7a4-5c68f94f6020" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_0c8516eb-d314-4dd3-aaeb-074463a60d50" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_0c8516eb-d314-4dd3-aaeb-074463a60d50_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_0c8516eb-d314-4dd3-aaeb-074463a60d50" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_0c8516eb-d314-4dd3-aaeb-074463a60d50_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_712d3a97-6190-4b3d-98fc-0d10d916a0a4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_0c8516eb-d314-4dd3-aaeb-074463a60d50" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_712d3a97-6190-4b3d-98fc-0d10d916a0a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_385dff32-dc78-4e6d-8426-30c07a12f203" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_712d3a97-6190-4b3d-98fc-0d10d916a0a4" xlink:to="loc_us-gaap_SubsequentEventMember_385dff32-dc78-4e6d-8426-30c07a12f203" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_d8b858e9-4af6-4653-b851-b1f1b84e0bed" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_ca897b1f-1cfa-4a2d-a7a4-5c68f94f6020" xlink:to="loc_us-gaap_DebtInstrumentAxis_d8b858e9-4af6-4653-b851-b1f1b84e0bed" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_d8b858e9-4af6-4653-b851-b1f1b84e0bed_default" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_d8b858e9-4af6-4653-b851-b1f1b84e0bed" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_d8b858e9-4af6-4653-b851-b1f1b84e0bed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_72e54bce-097c-460f-8ccb-9bf9e4284ab9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_d8b858e9-4af6-4653-b851-b1f1b84e0bed" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_72e54bce-097c-460f-8ccb-9bf9e4284ab9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2022Member_3d59c839-2dc5-43ee-9253-56c5757757e6" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2022Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_72e54bce-097c-460f-8ccb-9bf9e4284ab9" xlink:to="loc_xpo_SeniorNotesDue2022Member_3d59c839-2dc5-43ee-9253-56c5757757e6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_SeniorNotesDue2022Member_0df20efc-fcd4-428c-85c0-6ed78f885ace" xlink:href="xpo-20201231.xsd#xpo_SeniorNotesDue2022Member"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_f64c7724-d405-4e8e-af64-f4e6b6018ea1" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_ca897b1f-1cfa-4a2d-a7a4-5c68f94f6020" xlink:to="loc_dei_LegalEntityAxis_f64c7724-d405-4e8e-af64-f4e6b6018ea1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_f64c7724-d405-4e8e-af64-f4e6b6018ea1_default" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_f64c7724-d405-4e8e-af64-f4e6b6018ea1" xlink:to="loc_dei_EntityDomain_f64c7724-d405-4e8e-af64-f4e6b6018ea1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_a389b327-acb1-4d1e-8b5c-dd494fecdd4e" xlink:href="https://xbrl.sec.gov/dei/2020/dei-2020-01-31.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_f64c7724-d405-4e8e-af64-f4e6b6018ea1" xlink:to="loc_dei_EntityDomain_a389b327-acb1-4d1e-8b5c-dd494fecdd4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_xpo_KuehneNagelMember_76fc7440-942e-4ab2-a74c-f1beddf1fe87" xlink:href="xpo-20201231.xsd#xpo_KuehneNagelMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_a389b327-acb1-4d1e-8b5c-dd494fecdd4e" xlink:to="loc_xpo_KuehneNagelMember_76fc7440-942e-4ab2-a74c-f1beddf1fe87" xlink:type="arc" order="0"/>
  </link:definitionLink>
</link:linkbase>
